Inpp5d (NM_001110193) Mouse Untagged Clone
CAT＃: MC223682
Inpp5d (untagged) - Mouse inositol polyphosphate-5-phosphatase D (Inpp5d), transcript variant 3, (10ug)
CNY 14540.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | p150Ship; s-SHIP; SHIP; SHIP-1; SHIP1; SIP-145 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC223682 representing NM_001110193
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTGCCATGGTCCCTGGGTGGAACCATGGCAACATCACCCGCTCCAAGGCAGAGGAGCTACTTTCCA GAGCCGGCAAGGACGGGAGCTTCCTTGTGCGTGCCAGCGAGTCCATCCCCCGGGCCTACGCACTCTGCGT GCTGTTCCGGAATTGTGTTTACACTTACAGGATTCTGCCCAATGAGGACGATAAATTCACTGTTCAGGCA TCCGAAGGTGTCCCCATGAGGTTCTTCACGAAGCTGGACCAGCTCATCGACTTTTACAAGAAGGAAAACA TGGGGCTGGTGACCCACCTGCAGTACCCCGTGCCCCTGGAGGAGGAGGATGCTATTGATGAGGCTGAGGA GGACACTGTAGAAAGTGTCATGTCACCACCTGAGCTGCCTCCCAGAAACATTCCTATGTCTGCCGGGCCC AGCGAGGCCAAGGACCTTCCTCTTGCAACAGAGAACCCCCGAGCCCCTGAGGTCACCCGGCTGAGTCTCT CCGAGACACTGTTTCAGCGTCTACAGAGCATGGATACCAGTGGGCTTCCCGAGGAGCACCTGAAAGCCAT CCAGGATTATCTGAGCACTCAGCTCCTCCTGGATTCCGACTTTTTGAAGACGGGCTCCAGCAACCTCCCT CACCTGAAGAAGCTGATGTCACTGCTCTGCAAGGAGCTCCATGGGGAAGTCATCAGGACTCTGCCATCCC TGGAGTCTCTGCAGAGGTTGTTTGACCAACAGCTCTCCCCAGGCCTTCGCCCACGACCTCAGGTGCCCGG AGAGGCCAGTCCCATCACCATGGTTGCCAAACTCAGCCAATTGACAAGTCTGCTGTCTTCCATTGAAGAT AAGGTCAAGTCCTTGCTGCACGAGGGCTCAGAATCTACCAACAGGCGTTCCCTTATCCCTCCGGTCACCT TTGAGGTGAAGTCAGAGTCCCTGGGCATTCCTCAGAAAATGCATCTCAAAGTGGACGTTGAGTCTGGGAA ACTGATCGTTAAGAAGTCCAAGGATGGTTCTGAGGACAAGTTCTACAGCCACAAAAAAATCCTGCAGCTC ATTAAGTCCCAGAAGTTTCTAAACAAGTTGGTGATTTTGGTGGAGACGGAGAAGGAGAAAATCCTGAGGA AGGAATATGTTTTTGCTGACTCTAAGAAAAGAGAAGGCTTCTGTCAACTCCTGCAGCAGATGAAGAACAA GCATTCGGAGCAGCCAGAGCCTGACATGATCACCATCTTCATTGGCACTTGGAACATGGGTAATGCACCC CCTCCCAAGAAGATCACGTCCTGGTTTCTCTCCAAGGGGCAGGGAAAGACACGGGACGACTCTGCTGACT ACATCCCCCATGACATCTATGTGATTGGCACCCAGGAGGATCCCCTTGGAGAGAAGGAGTGGCTGGAGCT ACTCAGGCACTCCCTGCAAGAAGTCACCAGCATGACATTTAAAACAGTTGCCATCCACACCCTCTGGAAC ATTCGCATAGTGGTGCTTGCCAAGCCAGAGCATGAGAATCGGATCAGCCATATCTGCACTGACAACGTGA AGACAGGCATCGCCAACACCCTGGGAAACAAGGGAGCAGTGGGAGTGTCCTTCATGTTCAATGGAACCTC CTTGGGGTTCGTCAACAGCCACTTGACTTCTGGAAGTGAAAAAAAGCTCAGGAGAAATCAAAACTATATG AACATCCTGCGGTTCCTGGCCCTGGGAGACAAGAAGCTAAGCCCATTTAACATCACCCACCGCTTCACCC ACCTCTTCTGGCTTGGGGATCTCAACTACCGCGTGGAGCTGCCCACTTGGGAGGCAGAGGCCATCATCCA GAAGATCAAGCAACAGCAGTATTCAGACCTTCTGGCCCACGACCAACTGCTCCTGGAGAGGAAGGACCAG AAGGTCTTCCTGCACTTTGAGGAGGAAGAGATCACCTTCGCCCCCACCTATCGATTTGAAAGACTGACCC GGGACAAGTATGCATACACGAAGCAGAAAGCAACAGGGATGAAGTACAACTTGCCGTCCTGGTGCGACCG AGTCCTCTGGAAGTCTTACCCGCTGGTGCATGTGGTCTGTCAGTCCTATGGCAGTACCAGTGACATCATG ACGAGTGACCACAGCCCTGTCTTTGCCACGTTTGAAGCAGGAGTCACATCTCAATTCGTCTCCAAGAATG GTCCTGGCACTGTAGATAGCCAAGGGCAGATCGAGTTTCTTGCATGCTACGCCACACTGAAGACCAAGTC CCAGACTAAGTTCTACTTGGAGTTCCACTCAAGCTGCTTAGAGAGTTTTGTCAAGAGTCAGGAAGGAGAG AATGAAGAGGGAAGTGAAGGAGAGCTGGTGGTACGGTTTGGAGAGACTCTTCCCAAGCTAAAGCCCATTA TCTCTGACCCCGAGTACTTACTGGACCAGCATATCCTGATCAGCATTAAATCCTCTGACAGTGACGAGTC CTATGGTGAAGGCTGCATTGCCCTTCGCTTGGAGACCACAGAGGCTCAGCATCCTATCTACACGCCTCTC ACCCACCATGGGGAGATGACTGGCCACTTCAGGGGAGAGATTAAGCTGCAGACCTCCCAGGGCAAGATGA GGGAGAAGCTCTATGACTTTGTGAAGACAGAGCGGGATGAATCCAGTGGAATGAAATGCTTGAAGAACCT CACCAGCCATGACCCTATGAGGCAATGGGAGCCTTCTGGCAGGGTCCCTGCATGTGGTGTCTCCAGCCTC AATGAGATGATCAATCCAAACTACATTGCCAACCGAGGTCCCTGCCCCAGGGTGCAAGAGGCAAGACCTG GGGATCTGGGAAAGGTGGAAGCTCTGCTCCAGGAGGACCTGCTGCTGACGAAGCCCGAGATGTTTGAGAA CCCACTGTATGGATCCGTGAGTTCCTTCCCTAAGCTGGTGCCCAGGAAAGAGCAGGAGTCTCCCAAGATG CTGCGGAAGGAGCCCCCGCCCTGTCCAGACCCAGGAATCTCATCACCCAGCATCGTGCTCCCCAAAGCCC AAGAGGTGGAGAGTGTCAAGGGGACAAGCAAACAGGCCCCTGTGCCTGTCCTTGGCCCCACACCCCGGAT CCGCTCCTTTACCTGTTCTTCTTCTGCTGAGGGCAGAATGACCAGTGGGGACAAGAGCCAAGGGAAGCCC AAGGCCTCAGCCAGTTCCCAAGCCCCAGTGCCAGTCAAGAGGCCTGTCAAGCCTTCCAGGTCAGAAATGA GCCAGCAGACAACACCCATCCCAGCTCCACGGCCACCCCTGCCAGTCAAGAGTCCTGCTGTCCTGCAGCT GCAACATTCCAAAGGCAGAGACTACCGTGACAACACAGAACTCCCCCACCATGGCAAGCACCGCCAAGAG GAGGGGCTGCTTGGCAGGACTGCCATGCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001110193 |
| Insert Size | 3393 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001110193.2, NP_001103663.1 |
| RefSeq Size | 4757 bp |
| RefSeq ORF | 3393 bp |
| Locus ID | 16331 |
| UniProt ID | Q9ES52 |
| Gene Summary | Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways (By similarity). Able also to hydrolyzes the 5-phosphate of phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P3) and inositol 1,3,4,5-tetrakisphosphate (PubMed:9367159). Acts as a negative regulator of B-cell antigen receptor signaling. Mediates signaling from the FC-gamma-RIIB receptor (FCGR2B), playing a central role in terminating signal transduction from activating immune/hematopoietic cell receptor systems. Acts as a negative regulator of myeloid cell proliferation/survival and chemotaxis, mast cell degranulation, immune cells homeostasis, integrin alpha-IIb/beta-3 signaling in platelets and JNK signaling in B-cells. Regulates proliferation of osteoclast precursors, macrophage programming, phagocytosis and activation and is required for endotoxin tolerance. Involved in the control of cell-cell junctions, CD32a signaling in neutrophils and modulation of EGF-induced phospholipase C activity. Key regulator of neutrophil migration, by governing the formation of the leading edge and polarization required for chemotaxis. Modulates FCGR3/CD16-mediated cytotoxicity in NK cells. Mediates the activin/TGF-beta-induced apoptosis through its Smad-dependent expression.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) contains two alternate in-frame exons in the 3' coding region compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG226131 | Inpp5d (tGFP-tagged) - Mouse inositol polyphosphate-5-phosphatase D (Inpp5d) transcript variant 3, (10ug) |
CNY 12450.00 |
|
| MR226131 | Inpp5d (Myc-DDK-tagged) - Mouse inositol polyphosphate-5-phosphatase D (Inpp5d), transcript variant 3 |
CNY 11800.00 |
|
| MR226131L3 | Lenti ORF clone of Inpp5d (Myc-DDK-tagged) - Mouse inositol polyphosphate-5-phosphatase D (Inpp5d), transcript variant 3 |
CNY 13210.00 |
|
| MR226131L4 | Lenti ORF clone of Inpp5d (mGFP-tagged) - Mouse inositol polyphosphate-5-phosphatase D (Inpp5d), transcript variant 3 |
CNY 13210.00 |
