Gnas (NM_010309) Mouse Untagged Clone
CAT＃: MC223681
Gnas (untagged) - Mouse GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant 1, (10ug)
CNY 7896.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 5530400H20Rik; A930027G11Rik; C130027O20Rik; G; Ga; Galphas; Gn; Gnas1; Gnasxl; GPSA; Gs-; Gs-alpha; Gsa; GSP; N; Nes; Nesp; Nesp55; Nespl; Oed; Oed-Sml; Oedsml; P; P1; P2; P3; PHP1A; PHP1B; POH; SCG; SCG6; XL |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC223681 representing NM_010309.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGCATGTTCAACTGCCTCCACGGCAATAATATGTCAGGACAACACGATATCCCCCCTGAAGTCGGG GAGCAGCCCGAGCAAGAACCTTTGGAAGCCCCAGGGGCAGCTGCCCCCGGTGCTGGGGCTGGCCCAGCC GAAGAAATGGCGACCGAACCGGACTCCGAACCGTCTAACAATGAGCCCGTCCCCGACGAGACTGGCAGT GAGATCAGTGGACCCCCAGAAGACTCCAAATCTGACATCCAAAGCCCCTGCCAGGCCTTCGAGGAAGTC CGAGTGGGTGGAGACTACAGCCCACCTCCGGAGGAAGCCATGCCATTCGAGACACAACAGCCCAGCCTG GGAGATTTCTGGCCCACCCTGGAGCAGCCAGGACCATCTGGGACCCCATCAGGCCTCCAAGCCTTCAAC CCAGCGATTTTGGAGCCCGGGACCCCCACTGGCGCGAGCCCAGGCCTGGGAGCCTATACCCCCCCACCA GAAGAAGCTATGCCATTTGAGTTCAACGAGCCTGCCCAGGGAGACCATAGCCAGCCTCCCTTGCAAGTC CCAGACCTTGCGCCAGGAGGTCCGGAAGCATTGGTCCCCAGAGCTCTTCCCGCGGAGCCCGGGAACATC AGATTTGAAAACGCTGGCTTCCGAGAAGACTACAGCCCTCCCCCTGAAGAATCTGTGCCATTTCAGGTC GGTGGAGAAGAATTCGGGGGCGATAGCCCACCCCCAGGACTCCCGCGAGTCATCCCACAAATCGGCATT GGCGGCGAGTTCCCGACAGTCGCGGTCCCGAGTGCGCTCTGCCTCGCTCCCGCCGAGAACGCGCCTCCC CTCTGGGTCCGAGGCGCCATTGACAGACCATTCCGCGAGGCTGTCAGATCTCCTCCTAACTTCGCATGC GACAGCCCCCCGATGGAGATCACCAGACCCCTGCTTGAGATTGGCAGAGCCTCCATTGGGGTCGACGAC GACACCGCTGTCAATATGGACAGCCCCCCAATCGCAAGTGATGGCCCGCCCATCGAAGTCTCGGGAGCC CCAGATAAGAGCGAGTGCGCAGAGAGACCCCCAGTTGAGCGAGAAGCAGCCGAGATGGAAGGAAGCCCT ACCACCGCCACTGCGGTGGAAGGAAAAGTCCCCTCTCCGGAGAGAGGGGACGGATCTTCCACCCAGCCT GAAGCAATGGATGCCAAGCCAGCCCCTGCTGCCCAAGCCGTCTCTACCGGATCTGATGCTGGAGCTCCT ACGGATTCCGCGATGCTCACAGATAGCCAGAGCGATGCCGGAGAAGACGGGACAGCCCCAGGAACGCCT TCAGATCTCCAGTCGGATCCTGAAGAACTCGAAGAAGCCCCAGCTGTCCGCGCCGATCCTGACGGAGGG GCAGCCCCAGTCGCCCCAGCCACTCCTGCCGAGTCCGAGTCTGAAGGCAGCAGAGATCCAGCCGCCGAG CCAGCCTCCGAGGCAGTCCCTGCCACCACGGCCGAGTCTGCCTCCGGGGCAGCCCCTGTCACCCAGGTG GAGCCCGCAGCCGCGGCAGTCTCTGCCACCCTGGCGGAGCCTGCCGCCCGGGCAGCCCCTATCACCCCC AAGGAGCCCACTACCCGGGCAGTCCCCTCTGCTAGAGCCCATCCGGCCGCTGGAGCAGTCCCTGGCGCC CCAGCAATGTCAGCCTCTGCTAGGGCAGCTGCCGCTAGGGCAGCCTATGCAGGTCCACTGGTCTGGGGA GCCAGGTCACTCTCAGCTACTCCCGCCGCTCGGGCATCCCTTCCTGCCCGCGCAGCAGCTGCCGCCCGG GCAGCCTCTGCTGCCCGCGCAGTCGCTGCTGGCCGGTCAGCCTCTGCCGCCCCCAGCAGGGCCCATCTT AGACCCCCCAGCCCCGAGATCCAGGTTGCTGACCCGCCTACTCCGCGGCCTCCTCCGCGGCCGACTGCC TGGCCTGACAAGTACGAGCGGGGCCGAAGCTGCTGCAGGTACGAGGCATCGTCTGGCATCTGCGAGATC GAGTCCTCCAGTGATGAGTCGGAAGAAGGGGCCACCGGCTGCTTCCAGTGGCTTCTGCGGCGAAACCGC CGCCCTGGCCTGCCCCGGAGCCACACGGTCGGGAGCAACCCAGTCCGCAACTTCTTCACCCGAGCCTTC GGAAGCTGCTTCGGTCTATCCGAGTGTACCCGATCACGATCCCTCAGCCCCGGGAAGGCCAAGGATCCT ATGGAGGAGAGGCGCAAACAGATGCGCAAAGAAGCCATTGAGATGCGAGAGCAGAAGCGCGCAGATAAG AAACGCAGCAAGCTCATCGACAAGCAACTGGAGGAGGAGAAGATGGACTACATGTGTACACACCGCCTG CTGCTTCTAGGTGCTGGAGAGTCTGGCAAAAGCACCATTGTGAAGCAGATGAGGATCCTGCATGTTAAT GGGTTTAACGGAGAGGGCGGCGAAGAGGACCCGCAGGCTGCAAGGAGCAACAGCGATGGTGAGAAGGCC ACTAAAGTGCAGGACATCAAAAACAACCTGAAGGAGGCCATTGAAACCATTGTGGCCGCCATGAGCAAC CTGGTGCCCCCTGTGGAGCTGGCCAACCCTGAGAACCAGTTCAGAGTGGACTACATTCTGAGCGTGATG AACGTGCCGAACTTTGACTTCCCACCTGAATTCTATGAGCATGCCAAGGCTCTGTGGGAGGATGAGGGA GTGCGTGCCTGCTACGAGCGCTCCAATGAGTACCAGCTGATTGACTGTGCCCAGTACTTCCTGGACAAG ATTGATGTGATCAAGCAGGCCGACTACGTGCCAAGTGACCAGGACCTGCTTCGCTGCCGTGTCCTGACC TCTGGAATCTTTGAGACCAAGTTCCAGGTGGACAAAGTCAACTTCCACATGTTCGATGTGGGCGGCCAG CGCGATGAGCGCCGCAAGTGGATCCAGTGCTTCAATGATGTGACTGCCATCATCTTCGTGGTGGCCAGC AGCAGCTACAACATGGTCATTCGGGAGGACAACCAGACTAACCGCCTGCAGGAGGCTCTGAACCTCTTC AAGAGCATCTGGAACAACAGATGGCTGCGCACCATCTCTGTGATTCTCTTCCTCAACAAGCAAGACCTG CTTGCTGAGAAAGTCCTCGCTGGCAAATCGAAGATTGAGGACTACTTTCCAGAGTTCGCTCGCTACACC ACTCCTGAGGATGCGACTCCCGAGCCGGGAGAGGACCCACGCGTGACCCGGGCCAAGTACTTCATTCGG GATGAGTTTCTGAGAATCAGCACTGCTAGTGGAGATGGGCGCCACTACTGCTACCCTCACTTTACCTGC GCCGTGGACACTGAGAACATCCGCCGTGTCTTCAACGACTGCCGTGACATCATCCAGCGCATGCATCTC CGCCAATACGAGCTGCTCTAA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-RsrII |
| ACCN | NM_010309 |
| Insert Size | 3402 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_010309.4 |
| RefSeq Size | 3779 bp |
| RefSeq ORF | 3402 bp |
| Locus ID | 14683 |
| UniProt ID | Q6R0H7 |
| MW | 121.5 kDa |
| Gene Summary | This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contain a differentially methylated region (DMR) at their 5' exons, which is commonly found in imprinted genes and correlates with transcript expression. This gene has an antisense transcript. One of the transcripts produced from this locus, and the antisense transcript, are both paternally expressed noncoding RNAs, and may regulate imprinting in this region. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Additional transcript variants have been found for this gene, but the full-length nature and/or biological validity of some variants have not been determined. [provided by RefSeq, Jun 2015] Transcript Variant: This variant (1, also known as GNASXL) is paternally expressed. It includes an alternate 5' exon, compared to variant 7. This variant includes two overlapping open reading frames encoding distinct isoforms XLas and Alex, respectively. This RefSeq represents the isoform XLas (also known as alpha sXXL), which is a neuroendocrine-specific G-protein alpha subunit. It has a distinct and longer N-terminus, compared to isoform GNASL. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG225826 | Gnas (tGFP-tagged) - Mouse GNAS (guanine nucleotide binding protein alpha stimulating) complex locus (Gnas) transcript variant 1, (10ug) |
CNY 12450.00 |
|
| MR225826 | Gnas (Myc-DDK-tagged) - Mouse GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant 1 |
CNY 11310.00 |
|
| MR225826L3 | Lenti ORF clone of Gnas (Myc-DDK-tagged) - Mouse GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant 1 |
CNY 13210.00 |
|
| MR225826L4 | Lenti ORF clone of Gnas (mGFP-tagged) - Mouse GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant 1 |
CNY 13210.00 |
