Myo1e (NM_181072) Mouse Untagged Clone

CAT#: MC223584

Myo1e (untagged) - Mouse myosin IE (Myo1e), (10ug)



  "NM_181072" in other vectors (4)

CNY 14160.00


货期*
7周

规格
    • 10 ug

Product images

推荐一起购买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 2310020N23Rik; 9130023P14Rik; AA407778; myosin-1e; myr 3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC223584 representing NM_181072
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCAGCAAAGGCGCCTACCGGTACCATTGGCAGAGCCACAATGTCAAGCACAGTGGTGTGGACGACA
TGGTTCTACTGTCCAAGATCACAGAGAGCTCCATTGTAGAGAACCTGAAGAAGAGATACATGGACGACTA
TATTTTTACATATATAGGGTCTGTATTAATCTCTGTCAACCCATTCAAGCAAATGCCATATTTTGGAGAA
AAAGAAGTCGAAATGTACCAAGGAGCGGCACAGTATGAGAACCCGCCTCATATCTATGCCCTTGCTGATA
GTATGTACAGAAACATGATCATCGACAGAGAGAACCAGTGTGTCATCATCAGCGGTGAAAGTGGTGCTGG
AAAGACAGTGGCTGCCAAGTACATTATGAGCTATGTCTCCAGAGTGTCTGGAGGAGGCCCCAAGGTCCAG
CATGTGAAGGACATCATCCTTCAATCCAACCCGCTCCTGGAGGCCTTCGGAAACGCCAAGACAGTGCGCA
ACAACAACTCCAGCCGATTTGGAAAATACTTTGAAATCCAGTTCAGTCCAGGTGGGGAACCAGATGGTGG
CAAGATCTCCAACTTCCTTCTGGAAAAGTCCAGAGTGGTGATGCGGAACCCGGGGGAACGGAGCTTCCAC
ATATTCTACCAGCTCATTGAGGGTGCCTCTCCAGAGCAGAAGCAAAGCCTTGGCATCACCAGCATGGACT
ACTATTACTACCTGAGCCTCTCGGGGTCCTACAAGGTGGATGACATTGATGACAAGCGGGATTTCCAGGA
AACCCTGCACGCCATGAATGTGATTGGAATCTTCTCGGAAGAGCAGACTCTCGTGTTGCAGATTGTGGCT
GGAATTCTCCACTTGGGAAACATCAGCTTCAAAGAAGTGGGCAACTATGCCGCCGTGGAGAGTGAAGAAT
TTTTGGCTTTTCCTGCGTATCTGCTGGGTATAAACCAAGACCGGTTAAAAGAGAAGCTAACGAGCCGCCA
GATGGACAGTAAGTGGGGAGGAAAGTCAGAGTCCATCCATGTGACCCTCAACGTGGAGCAGGCCTGCTAC
ACCAGGGACGCGCTCGCCAAGGCCCTGCACGCACGAGTCTTTGATTTCTTGGTAGATTCTATCAATAAAG
CCATGGAGAAGGACCACGAGGAGTACAACATTGGAGTCCTGGACATCTATGGCTTTGAAATATTTCAGAA
AAATGGCTTTGAGCAGTTCTGCATCAATTTCGTTAACGAAAAATTACAGCAGATTTTTATTGAGCTGACG
CTAAAAGCAGAGCAGGAAGAATACGTTCAGGAGGGAATAAGATGGACACCCATTGAATATTTCAATAATA
AAATCGTGTGTGACCTCATAGAGAGCAAAGTCAACCCTCCTGGGATTATGAGCATCCTGGATGACGTGTG
CGCCACCATGCATGCTGTGGGTGAGGGGGCTGACCAGACTCTGCTACAGAAGCTCCAGATGCAGATCGGG
AGCCATGAGCACTTCAACAGCTGGAACCAAGGCTTCATCATTCACCACTACGCTGGGAAGGTTTCCTATG
ACATGGACGGCTTCTGCGAAAGGAACCGGGATGTGCTTTTCATGGACCTGATTGAACTCATGCAGAGCAG
TGAGCTGCCTTTTATAAAGTCTTTATTTCCTGAAAACCTGCAAGCTGACAAGAAAGGGCGCCCAACGACC
GCAGGAAGCAAAATAAAGAAACAAGCCAACGACCTGGTGAGCACCCTGATGAAATGCACACCCCACTACA
TTCGCTGCATAAAACCAAACGAAACCAAGAAGCCTAAAGACTGGGAGGAGAGCAGAGTGAAGCATCAAGT
GGAGTACCTGGGTCTCAAAGAAAACATCCGAGTGAGAAGAGCTGGTTATGCTTATCGGCGTGTCTTCCAG
AAGTTTCTACAGAGGTATGCCATTCTGACCAAAGCTACCTGGCCTGTGTGGAGAGGAGATGAGAAACAAG
GTGTCCTCCATCTACTGCAGTCTGTCAACATGGACAGCGACCAGTTCCAGCTTGGGAGAAGTAAAGTGTT
CATCAAAGCCCCAGAGTCTCTGTTTCTTCTAGAAGAGATGAGAGAGAGAAAGTACGATGGTTACGCACGT
GTGATACAGAAAACATGGAGGAAGTTCGTGGCCCGCAAGAAATATGTTCAAATGAGAGAAGAAGCCTCAG
ACCTGCTGTTGAACAAGAAGGAGAGGAGGAGAAACAGCATCAACCGAAACTTCATCGGGGACTACATCGG
GATGGAGGAGCGCCCGGAACTGCAGCAGTTTGTAGGCAAGAGGGAGAAGATTGACTTTGCTGACACAGTC
ACCAAGTACGACAGGAGGTTCAAGGGTGTCAAGCGAGACCTGCTGCTAACCCCCAAGTGCTTGTACTTAA
TTGGAAGGGAGAAGGTGAAGCAAGGCCCAGACAAAGGTGTTGTGAAGGAAGTCCTGAAGCGGAGGATCGA
GGTGGAAAGGATCCTGTCCGTGTCCCTCAGTACCATGCAGGATGACATCTTCATTCTCCACGAGCAAGAA
TATGACAGTTTGCTGGAGTCCGTCTTTAAAACGGAGTTCCTGAGCCTCTTAGCAAAGCGATACGAGGAAA
AAACCCAGAAGCAACTACCTCTGAAATTCAGCAATACCCTTGAACTGAAGTTGAAAAAGGAGAACTGGGG
TCCCTGGAGTGCAGGGGGCTCCCGGCAAGTGCAGTTCCACCAAGGCTTCGGTGACCTGGCTATCCTCAAG
CCCAGCAACAAAGTGCTGCAGGTCAGCATCGGACCCGGGCTGCCCAAGAACTCCCGTCCTACCAGAAGAA
ACACCGTCACCAGCAGAGGGTATCCAGGTGGAACTAAGAACAATTACCCGATGAGAGCTGCCCCTGCTCC
CCCAGGATGCCATCAAAACGGAGTCATACGAAACCAGTTTGTGCCTCCTCCCCATGCCTTTGGAAACCAG
AGGTCCAATCAGAAAAGCCTGTACACCTCTATGGCCCGCCCACCCTTGCCAAGGCAACAGTCCACCGGCT
CGGATCGATTGTCTCAGACGCCAGAGAGCCTGGATTTCCTCAAGGTCCCGGACCAGGGTGTTGCTGGAGT
CCGAAGACAAACGAGCAGCCGGCCTCCCCCAGCAGGGGGCAGACCAAAGCCTCAACCCAAGCCTAAGCCT
CAGGTGCCACAGTGCAAGGCCTTGTATGCCTATGATGCTCAGGACACGGACGAACTCAGCTTCAACGCCA
ACGATATTATTGATATTATTAAAGAAGACCCTTCTGGCTGGTGGACGGGTAGACTACGAGGCAAGCAGGG
CCTGTTTCCTAACAACTACGTCACCAAGATCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_181072
Insert Size 3324 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_181072.3, NP_851417.2
RefSeq Size 4944 bp
RefSeq ORF 3324 bp
Locus ID 71602
UniProt ID E9Q634
Gene Summary Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails bind to membranous compartments, which are then moved relative to actin filaments. Binds to membranes containing anionic phospholipids via its tail domain (By similarity). Required for normal morphology of the glomerular basement membrane, normal development of foot processes by kidney podocytes and normal kidney function. In dendritic cells, may control the movement of class II-containing cytoplasmic vesicles along the actin cytoskeleton by connecting them with the actin network via ARL14EP and ARL14 (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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