Emc1 (NM_146157) Mouse Untagged Clone
CAT＃: MC223044
Emc1 (untagged) - Mouse RIKEN cDNA C230096C10 gene (C230096C10Rik), transcript variant 1, (10ug)
CNY 12920.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 2700016F22Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC223044 representing NM_146157
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGTCGCGGTGGCGTCTGGGTTCTGGATCTGGGCTGCTGTGCTGCTCGTCCCTGCGGCCGCGGTCT ACGAAGACCAAGTGGGCAAGTTTGACTGGAGACAGCAGTATGTTGGGAAGATCAAGTTTGCCTCCTTGGA GTTTTCCCCTGGATCCAAGAAGTTGGTTGTGGCCACAGAGAAGAATGTGATTGCGGCGTTAAATTCTCGG ACTGGAGAGATCTTGTGGCGCCATGTTGACAAAGGCACAGCAGAAGGAGCTGTGGATGCCATGCTGGTCC ATGGACAAGATGCAATCACTGTATCCAACGGAGGGCGCCTCATGCGTTCCTGGGAGACCAACATCGGGGG CCTGAACTGGGAGATAACTCTGGACACTGGCAGTTTCCAGGCACTTGGGCTAGTGGGCCTTCAGGAGTCA GTGAGGTACATTGCAGTTCTGAAGAAGACGACCCTCACCCTCCACCACCTCTCCAGTGGGCACCTGAAGT GGGTGGAGCATCTGCCAGAAAGTGACAGCATCCTCTACCAGATGGTGTATTCCTACGGCTCCGGGGTGGT GTGGGCCCTTGGCATCGTTCCCTTCAGCCACGTGAACATCGTCAAGTTTAACGTGGAGGATGGAGAGATT GTTCAGCAGGTCAGGGTTTGGACTCCATGGCTGCAGCACCTCACTGGGGCCTGTGGTGTGGTAGATGAGG CTGTCCTGGTATGCCCCGACCCCAGCTCACACTCACTCCACACTTTGGCCCTGGAGACGGAGTGGGAACT GCGACAGATCCCCCTGCAGTCTCCTGACTTAGAATTTGGAAGTGGATTCCAGCCCCAAGTGCTGCCCACA CAACCCAGCCCAGTGGCTCCTTCTCGAGCCCAGTTCTTCCTGCAGTTGTCCCCAAGCCACTACGCTCTAC TGCACTACCACCATGGTGCCGTGACTCTGCTTAAGAACTTCCCCCAGGCCACGCTCGTCAGCTTCGCCAC CACAGGGGAGAAGACAGTGGCTGCAGTCATGACCTGTCGCACTGAAGTGCAAAAACCTGTCAGTGCTGGA GATGGGTCTGTGGCAAGCTTTCCTGAGACATCTGGTGCACAGGACTCTCTGGCTTGCTTCAACCAGACCT ACACCATTAACCTGTACTTAGTGGAGACAGGTCGGCGGCTCCTAGACACTTCCATTTCCTTCAGCCTGGA GCAGAAGGGCACGCGACCTGAGCAGCTGTACATCCAGGTGTTCTTGAAGAAGGACGACTCGGTGGGCTAC CGGGCCTTGGTGCAGACACAGGACCATCTGCAGCTGTTCCTGCAGCAGCTTGCTGGGAAGGTGGTGTTGT GGAGCCGAGAGGAGTCCCTGGCAGAAGTCGTGTGCCTGGAGATGGTGGATCTCCCCCTGACTGGAGCGCA GGCTGAGTTGGAAGGAGAATTTGGCAAGAAGGCAGCGATTCAAGATGGCTTGCTGGGGATGTTCCTGAAA CGCCTGTCGTCCCAGCTCATCCTGCTCCAGGCCTGGACTTCCCACCTCTGGAAAATGTTCTACGATGCTC GGAAGCCCCGAAGCCAGATTAAGAATGAGATCAACATCGACACACTGGCTAGAGATGAGTTCAACCTGCA GAAAATGATGGTGACGGTAACAGCCTCAGGCAAGCTCTTTGGCATCGAGAGCAGCTCTGGAACCATTCTG TGGAAGCAGTATCTGCCCAATGTTAAGCCAGACTCCTCCTTTAAGCTGATGGTGCAGAGGACTACTGCCC ATTTCCCCCATCCACCCCAGTGCACGCTGCTGGTGAAGGACAAGGAGACAGGAATGAGCTCTCTGTTTGT CTTCAACCCCATTTTTGGCAAGTGGAGCCAGGTAGCTCCCCCAGTGCTGAAGCGCCCCATCTTGCAGTCA CTGCTTCTCCCAGTCATGGATCAAGACTATGCCAAGGTGTTGCTGTTGGTGGATGACGAATACAAGGTCA CAGCTTTCCCAGCTACGCGGAATGTCTTGCGGCAGCTGCATGAACTTGCCCCCTCCATCTTCTTCTACTT GGTGGATGCAGAGCAGGGGCGGCTCAGTGGGTATCAGCTTCGAAAGGATCTCACTACAGAACTGAGTTGG GAGCTGACCATCCCCCCAGAAGTGCAGCGCGTCGTCAAGGTCAAGGGGAAGCGCAGCAGCGAGCACGTAC ACTCCCAGGGCCGGGTGATGGGGGATCGAAGTGTGCTCTACAAGAGTCTGAACCCCAACTTGCTGGCCGT GGTGACAGAGAGCACGGATGTCCACCACGAGCGCACCTTTATTGGCATCTTCCTCATTGATGGTGTCACT GGCCGCATCATCCACTCCTCCGTGCAGAAGAAGGCCAGGGGCCCTGTCCACCTTGTGCATTCAGAGAACT GGGTGGTGTACCAGTACTGGAATTCTAAGGCTCGGCGCAATGAGCTGACGGCCCTGGAGCTCTACGAGGG CACTGAACAGTACAATGCCACTGCCTTCAGCTCTCTAGACCGACCCCAGCTGCCCCAGGTCCTCCAGCAG TCCTACATCTTCCCCTCCTCCATCAGTGCCATGGAAGCTACCATCACCGAGAGGGGCATCACCAGCCGGC ACTTGCTCATTGGGCTGCCTTCTGGAGCAATCCTTTCCCTCCCCAAGGCCTTGCTGGATCCCCGGCGCCC AGAGATCCCAACAGAGCAAAGCAGAGAAGAGAACCTGATCCCATATTCTCCAGATGTTCAGGTCCATGCA GAGCGATTCATCAACTATAACCAGACAGTCTCTCGAATGCGAGGCATCTATACAGCGCCCTCAGGCCTGG AGTCCACTTGTTTGGTTGTGGCCTACGGTTTGGATATTTACCAAACTCGAGTTTACCCGTCCAAGCAGTT TGATGTCCTGAAAGATGACTACGACTATGTGCTCATCAGCAGTGTCCTTTTTGGCCTGGTTTTTGCCACC ATGATCACAAAGAGGCTGGCACAGGTGAAACTCCTCAATCGAGCCTGGCGGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_146157 |
| Insert Size | 2994 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_146157.4, NP_666269.2 |
| RefSeq Size | 6237 bp |
| RefSeq ORF | 2994 bp |
| Locus ID | 230866 |
| UniProt ID | Q8C7X2 |
| Gene Summary | Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins. Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues. Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices. It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes. By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N-terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors. By regulating the insertion of various proteins in membranes, it is indirectly involved in many cellular processes.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG217459 | Emc1 (tGFP-tagged) - Mouse RIKEN cDNA C230096C10 gene (C230096C10Rik) transcript variant 1, (10ug) |
CNY 11020.00 |
|
| MR217459 | Emc1 (Myc-DDK-tagged) - Mouse RIKEN cDNA C230096C10 gene (C230096C10Rik), transcript variant 1 |
CNY 9980.00 |
|
| MR217459L3 | Lenti ORF clone of Emc1 (Myc-DDK-tagged) - Mouse RIKEN cDNA C230096C10 gene (C230096C10Rik), transcript variant 1 |
CNY 11880.00 |
|
| MR217459L4 | Lenti ORF clone of Emc1 (mGFP-tagged) - Mouse RIKEN cDNA C230096C10 gene (C230096C10Rik), transcript variant 1 |
CNY 11880.00 |
