Reck (NM_016678) Mouse Untagged Clone
CAT＃: MC222903
Reck (untagged) - Mouse reversion-inducing-cysteine-rich protein with kazal motifs (Reck), (10ug)
CNY 12540.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | mRECK; St15 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC222903 representing NM_016678
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGAGCGTCCGGGCCTCCCCGCGCAGCGCGCTGCTACTTCTGCTGGCCGCGGCGGGGGTCGCGGAGG TGACGGGGGGCCTGGCCCCGGGCAGCGCGGGTGCTGTATGCTGTAATCATTCAAAAGATAACCAAATGTG CCGTGATGTATGTGAGCAGATTTTCTCCTCAAAAAGTGAATCCCGACTGAAACATCTGTTGCAGAGAGCC CCAGATTATTGCCCTGAAACAATGGTTGAAATTTGGAGTTGCATGAATTCGTCTTTGCCAGGGGTATTTA AGAAGTCCGATGGTTGGGTTGGCTTAGGCTGCTGTGAACTGGCTATTGGCTTGGAGTGCCGACAGGCATG CAAGCAGGCATCTTCGAAAAATGATATTTCCAAAGTTTGCAGGAAAGAATATGAGAATGCTCTTTTCAGT TGCATTAGCAGAAATGAAATGGGCTCGGTGTGCTGCAGTTACGCAGGCCATCACACCAACTGCCGAGAAT TCTGTCAAGCCATTTTTCGAACAGACTCTTCTCCTGGGCCATCTCAGATCAAAGCAGTGGAAAATTATTG TGCCTCTATCAGTCCACAGTTGATACACTGTGTGAATAATTACACCCAGTCTTATCCAATGAGAAACCCA ACTGATAGTTTATATTGCTGTGACAGAGCTGAAGACCATGCTTGCCAAAACGCCTGCAAGAGAATTCTAA TGTCTAAGAAAACAGAAATGGAGATTGTTGATGGTCTCATTGAGGGTTGTAAGACCCAGCCCTTGCCTCA AGATCCTCTTTGGCAGTGTTTTCTCGAAAGCTCACAGTCGGTTCACCCTGGAGTCACTGTGCACCCTCCT CCCTCAACTGGCCTCGACGGGGCCAAATTGCACTGTTGCTCTAAAGCAAACACTTCAACCTGTAGGGAAC TGTGCACCAAACTTTATAGCATGAGCTGGGGAAATACACAGAGTTGGCAAGAGTTTGACCGCATCTGTGA GTATAATCCGGTGGAAGTGTCCATGCTGACGTGTCTGGCTGATGTCCGGGAGCCTTGCCAGTTGGGCTGT ACAAACCTGACTTACTGTACAAACTTTAACAACAGGCCAACAGAACTGTTCAGGAGCTGCACGGCTCAGT CTGACCAGGGGGCCATGAGTGACATGAAGCTGTGGGAGAAGGGGAGCATCAAGATGCCCTTCATCAGCAT CCCTGTCCTAGACATCAAGACCTGCCAGCCAGAGATGTGGAAGGCCGTGGCCTGCTCCCTGCAGATCAAG CCGTGCCATAGCAAATCCCGAGGCAGTATTATCTGCAAATCAGATTGTGTAGAGATTCTCAAGAAATGTG GAGACCAGAATAAATTCCCTGAAGAACACACAGCTGAAAGTATCTGTGAGTTTCTGTCCCCTGCAGATGA CCTGGAGAGCTGTATCCCTCTGGACACGTACCTCAGGCCAAGTGCTTTAGGAAACATCATAGAAGAAGTG ACTCACCCCTGTAACCCAAACCCCTGCCCAGCCAACGAGCTCTGTGAAGTGAACCGGAAGGGGTGTCCGT CTGCAGATCCCTGCCTTCCATACTCCTGTGTTCAAGGCTGCAAATTGGGAGAAGCTTCTGACTTCATCGT CCGCCAGGGGACACTGATCCAGGTGCCGTCCTCTGCAGGGGAAGTTGGTTGCTACAAGATCTGTTCCTGT GGACAAAGTGGCCTCTTGGAAAACTGTATGGAAATGCACTGTATCGACCTCCAGAAGTCTTGTATTGTCG GAGGAAAAAGAAAAAGCCACGGAACATCCTTTACTATTGACTGCAATGTCTGTTCTTGTTTTGCGGGTAA TTTAGTGTGTTCCACCCGCCTGTGCCTCAGTGAGCACAGTTCAGACGACGACCGCCGCACCTTCACAGGT CTGCCCTGTAACTGCGCAGATCAGTTTGTCCCAGTGTGTGCACAGAATGGACGCACTTACCCCAGTGCCT GCATCGCTCGCTGTGTGGGTCTCCAGGACCATCAGTTTGAGTTTGGACCTTGTATCTCCAAGAATCCCTG TAATCCTAACCTCTGCCCCAAAAGCCAAAGATGTGTGCCAAAACCACAAGTCTGCCTGACGACTTTTGAT AAATTTGGATGTAGCCAATATGAGTGTGTGCCCAGACAGCTCACCTGTGACCAGGCCCGAGATCCTGTGT GTGACACAGATCACATGGAGCACAGCAACCTCTGCACTCTGTACCAAAGAGGGAAAAGCCTGTCTTACAG AGGCCCTTGCCAGCCTTTCTGCAGAGCCAAGGAGCCTGTCTGTGGGCACAATGGTGAGACATACAGCAGT GTGTGTGCTGCCTACTCAGATCGTGTGGCCGTGGATTACTACGGGCCCTGCCAGGCTGTCGGCGTCCTCT CGGAATACAGTGCTGTGGCTGAGTGTGCTGCTGTCAAGTGTCCTTCCCTCTCAGCCATAGGCTGCAAACC CATCATCCCACCAGGGGCTTGCTGTCCACTATGTGCTGGGATGTTACGGGTATTATTTGACAAAGAAAAA CTGGACACTATTGCTAAGGTGACAAGCAAGAAGCCCATAACCGTTGTGGAGATTCTTCAGAAGGTCCGCA TGCACGTGTCTGTGCCGCAGTGTGACGTGTTTGGATACTTGAGCATCGAGTCGGAAATTGTGATCCTCAT TATTCCTGTGGACCATTACCCAAAAGCTTTGCAGATTGAGGCCTGCAATAAGGAGGCAGAGAAAATCGAG TCCCTCATCAACTCCGACAGCCCCACGCTGGCGTCCCACGTCCCACTCTCGGCCCTCATCATTTCCCAAG TGCAGGTCTCCAGCAGTCTCCCGTCATCTGCTGTTGTGGGCAGGCCTCTCTTCCACTCCCTGCTCCTCCT CCTCAGCCTGGGCCTCACCGTGCACTTGCTCTGGACTAGACCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_016678 |
| Insert Size | 2916 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_016678.2, NP_057887.2 |
| RefSeq Size | 4450 bp |
| RefSeq ORF | 2916 bp |
| Locus ID | 53614 |
| UniProt ID | Q9Z0J1 |
| Gene Summary | Functions together with ADGRA2 to enable brain endothelial cells to selectively respond to Wnt7 signals (WNT7A or WNT7B) (PubMed:28803732). Plays a key role in Wnt7-specific responses: required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation (PubMed:26658478, PubMed:28803732). Acts as a Wnt7-specific coactivator of canonical Wnt signaling by decoding Wnt ligands: acts by interacting specifically with the disordered linker region of Wnt7, thereby conferring ligand selectivity for Wnt7 (By similarity). ADGRA2 is then required to deliver RECK-bound Wnt7 to frizzled by assembling a higher-order RECK-ADGRA2-Fzd-LRP5-LRP6 complex (By similarity). Also acts as a serine protease inhibitor: negatively regulates matrix metalloproteinase-9 (MMP9) by suppressing MMP9 secretion and by direct inhibition of its enzymatic activity (PubMed:11747814). Also inhibits metalloproteinase activity of MMP2 and MMP14 (MT1-MMP) (PubMed:11747814).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG227392 | Reck (tGFP-tagged) - Mouse reversion-inducing-cysteine-rich protein with kazal motifs (Reck), (10ug) |
CNY 7890.00 |
|
| MR227392 | Reck (Myc-DDK-tagged) - Mouse reversion-inducing-cysteine-rich protein with kazal motifs (Reck) |
CNY 7112.00 |
|
| MR227392L3 | Lenti ORF clone of Reck (Myc-DDK-tagged) - Mouse reversion-inducing-cysteine-rich protein with kazal motifs (Reck) |
CNY 9030.00 |
|
| MR227392L4 | Lenti ORF clone of Reck (mGFP-tagged) - Mouse reversion-inducing-cysteine-rich protein with kazal motifs (Reck) |
CNY 9512.00 |
