Col9a1 (NM_007740) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Col9a-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC222624 representing NM_007740 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGAACTTCTGGAAAATCTCAGTTTTCTTCTGTGTGTGCAGTTGTCTGGGACCCTGGGTATCCGCAA CTCTTAAGCGTCGTGCAAGATTTCCTGCCAATTCCATTTCTAATGGTGGAAGTGAACTGTGTCCCAAGAT CAGGATTGGCCAAGATGACTTACCAGGGTTTGACCTGATTTCTCAGTTCCAAATTGAGAAGGCTGCATCT CGAAGGACTATCCAGAGGGTGGTGGGATCCACAGCCTTACAAGTGGCTTACAAGCTGGGAAGTAATGTAG ACTTCAGGATTCCAACAAGGCATTTGTATCCCAGTGGACTGCCTGAAGAATATTCCTTTTTAACTACTTT CCGGATGACTGGAAGCACACTTGAAAAGCACTGGAACATTTGGCAGATCCAGGATTCCGCAGGGAGAGAG CAAGTTGGCGTGAAGATAAATGGCCAAACAAAGTCTGTCGCATTTTCATACAAGGGACTGGATGGGAGTC TCCAAACGGCTGCCTTCTTGAATTTGCCGTCCTTGTTTGACTCCCGGTGGCATAAGCTCATGATTGGCGT GGAAAGAACAAGCGCCACTCTTTTTATTGACTGCATCAGGATCGAATCTTTACCTATAAAGCCAAGAGGC CAGATTGATGCGGATGGCTTTGCAGTGCTGGGAAAACTTGTGGACAATCCTCAGGTTTCTGTTCCTTTTG AACTCCAGTGGATGCTGATTCATTGTGACCCCCTGAGACCCAGGAGAGAAACCTGTCATGAGCTGCCAAT CAGAATCACAACCAGCCAGACCACTGATGAGAGAGGTCCTCCGGGTGAGCAGGGGCCTCCAGGGCCTCCT GGGCCTCCTGGAGTTCCGGGCATAGATGGCATTGATGGTGACCGAGGTCCAAAGGGTCCCCCAGGACCTC CGGGTCCTCCTGGAGACCCAGGCAAGCCAGGAGCACCAGGCAAGCCAGGCACACCAGGAGCTGATGGATT AACAGGACCTGATGGATCCCCTGGCTCTGTTGGACCAAGGGGACAAAAAGGAGAACCTGGTGTACCTGGG TCTCGTGGATTTCCAGGCCGTGGTATTCCAGGACCCCCTGGTCCTCCTGGGACCACAGGACTTCCTGGAG AACTTGGCCGAGTAGGCCCTATTGGAGACCCTGGGAAAAGAGGACCACCTGGCCCTCCTGGACCTCCAGG ACCCAGTGGAACAATTGGATTTCATGATGGAGACCCATTGTGCCCCAATTCCTGCCCACCAGGTCGCTCT GGATATCCAGGCCTACCAGGCATGAGGGGCCATAAAGGGGCGAAAGGAGAAATCGGCGAGCCAGGAAGAC AAGGACACAAGGGTGAAGAGGGTGACCAGGGGGAACTGGGAGAAGTTGGCGATCAAGGACCTCCAGGACC TCAGGGTCTGAGAGGCATCACTGGCATAGTTGGAGACAAAGGAGAAAAGGGTGCTCGGGGATTTGATGGA GAGCCTGGACCTCAGGGCATTCCAGGTGCAGCTGGTGATCAAGGACAGCGAGGCCCTCCAGGAGAAACAG GTCCTGAGGGAGACAGAGGCATTCAAGGTTCCCGAGGAATTCCTGGATCCCCAGGGCCAAAAGGAGACAC GGGCTTGCCAGGTGTAGATGGCCGAGATGGAATACCAGGAATGCCCGGGACAAAGGGTGAAGCAGGGAAG CCTGGACCCCCCGGGGACGTGGGATTGCAGGGCTTACCAGGTGTCCCTGGAATCCCTGGTGCAAAAGGTG TTGCCGGTGAAAAGGGTAACACGGGTGCTCCAGGAAAGCCCGGTCAGTTGGGAAGTTCAGGGAAACCAGG CCAACAAGGGCCCCCAGGAGAGGTTGGACCTCGGGGACCCAGGGGCCTTCCAGGCAGTAGAGGCCCGGTA GGACCAGAAGGGTCTCCAGGCATACCAGGGAAACTGGGATCTGTTGGCAGCCCTGGCCTTCCTGGCTTGC CTGGGCCCCCTGGACTTCCTGGAATGAAAGGAGACAGGGGTGTATTTGGTGAACCGGGTCCCAAGGGTGA ACAGGGTGCCTCTGGTGAAGAAGGTGAAGCAGGAGCAAGGGGTGACCTTGGAGATATGGGACAACCTGGC CCAAAGGGATCTGTGGGTAACCCCGGGGAGCCGGGTCTGAGGGGGCCTGAAGGAATCAGAGGGCTTCCTG GAGTGGAAGGACCAAGAGGACCACCTGGACCCCGGGGCATGCAGGGAGATCAGGGTGCCACTGGGCTGCC TGGTATCCAGGGCCCTCCGGGCAGAGCGCCGACCGACCAGCACATCAAGCAGGTTTGCATGAGAGTCGTG CAAGAGCATTTTGCGGAAATGGCAGCCAGCCTCAAGAGACCAGACACAGGAGCCTCTGGTCTTCCTGGGA GGCCTGGCCCCCCTGGGCCTCCGGGCCCCCCTGGAGAGAATGGTTTCCCTGGTCAGATGGGAATCCGTGG TCTCCCAGGCATTAAGGGTCCCCCTGGTGCTCTTGGCTTAAGAGGACCTAAAGGAGACTTGGGAGAAAAA GGAGAACGTGGTCCTCCAGGAAGAGGTCCTAAGGGTTTGCCTGGAGCGATAGGTCTCCCAGGTGACCCAG GCCCTGCCAGCTATGGGAAAAATGGCCGTGACGGAGAGCAAGGTCCCCCGGGAGTGGCAGGAATTCCTGG TGTGCCTGGACCCCCAGGTCCTCCAGGCCCTCCTGGGTTCTGTGAGCCAGCCTCTTGCACCCTGCAGTCT GGTCAAAGAGCATTTAGCAAAGGGCCGGACAAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_007740
Insert Size	2766 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_007740.3, NP_031766.3
RefSeq Size	3888 bp
RefSeq ORF	2766 bp
Locus ID	12839
UniProt ID	Q05722
Gene Summary	Structural component of hyaline cartilage and vitreous of the eye.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.