Gen1 (NM_177331) Mouse Untagged Clone
CAT＃: MC222548
Gen1 (untagged) - Mouse Gen homolog 1, endonuclease (Drosophila) (Gen1), (10ug)
CNY 11690.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 5830483C08Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC222548 representing NM_177331
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGTGAATGACTTATGGCAAATTCTAGAGCCTGTAAAGCAACATATCCACTTGCAAGATCTTAGTG GGAAAACCATTGCCGTTGATTTGAGTCTCTGGGTATGCGAGGCACAGACAGTGAAGAAAATGATAGGAAC TGTCAAGAAGCCCCACCTCAGGAACCTATTTTTTCGTATTTCATATTTAACTCAAATGAATGTAAAACTG GTGTTTGTAATGGAAGGGGAGCCACCAATGCTGAAAGCTGATGTCATAAGCAAGAGGACTCAGACTCGTT ACGGGCCTTCTGGAAAATCAAGGTCTCAGAAAACAGGGAGATCACATTTTAAGTCAGTCTTACGAGAGTG CCTTGAAATGCTCGAGTGCCTGGGAATGCCCTGGGTCCAGGCCGCCGGGGAAGCTGAGGCCATGTGTGCT TACCTTAATGCCAGTGGCCATGTGGATGGCTGCCTCACCAACGACGGCGATGCTTTCCTTTATGGAGCCC AGACTGTTTACAGAAATTTCACGATGAACACAAAGGATCCCCATGTTGACTGTTACACGATATCATCTAT CAAGAGTAAGCTAGGCTTGGATCGAGACGCTCTGGTTGGACTGGCCGTACTTCTGGGTTGTGATTACCTT CCAAAGGGAGTGCCTGGAGTTGGAAAGGAACAAGCATTAAAACTTTTGCAGATTTTCAAAGGTCAAAGTT TGCTTCAGAGGTTTAACCAGTGGATTGAAGACCCTTGCTACTCTGTTCCACAATCAGCACCAAAAAAAGT GGTTCACTGCTCTGTGTGTTCCCACCCAGGTTCGCCAAAGGATCATGAACGTAATGGATGCATATTATGT AAAAGTGATAAATACTGTGAACCACATGATTATGATTACCTCTGCCCTTGTGAATGGCATCAGACAGACC ATAATAGGCATCTAAGTGAAATAGAAAACAATATTAAAAAGAAAGCTTGCAGTTGCGAAGGATTTCCATT CCATGAGGTCATCCAAGAGTTCCTTTTGAATAAGAATAAAATGTTGAAACCGATCACATACCAAAGACCT GATTTATTATTGTTTCAGAGATTTACTGTTCAAAAAATGGAGTGGCCCAGTCACTATGCATGTGAAAAAT TGTTGGTGCTTTTGACCCGCTATGACATGATAGAAAGAAAACATGGTAGAAAGACCTCCAATCAACTACA GCCAATTAGAATTGTTAAACCCCGAGTCAGAAATGGAGTCCATTGTCTTGAAATAGAATGGGAAAAGCCT GAACATTATGTGGTGGAAGATGGAGACCCTGGAAAACTGAGCCTCCTTACAATGGAAGAAGCGTCCCTGT TTGAAGCAGCCTATCCTGATGCTGTGGCTGTCTACCAGAAACAGCTGTCAGAGACCAAAGGAAGGAAACA GAAAAGTATGAAAAATAAGCCTAAAGGAAGCCATTTACCGGAAGCAGATGATGTGATCAATTCTCAGTCA CTTATGACTTTAAAACCTACATCTAAAGCCTTCCCCAAGCAAAATCCCAAAATTAATTTGGAAAATTCTC CAGATCCTATTTTAGCACAGGAATCTACTTCCCCCTCATTGAATAGTTTTGTTTCCCCTGAAAATGCTCC CTGTTTGAATTTACAAGAACAGTTAGTGCCATCTCCCAGAACTTTGGCTATAAAGCAAAGTAAAGATGTC AGTCATTTTCTAGTTTCAGAATGTAGTCAGCCCAGCTCTTCCTCCCATGACATCTCTGTGATTACTGATC TGCAGTTGAGTACCATTGATTGGGCGGGTACTTCTTTTAGTAATTCTCCAGCAGTTCAAAGAAACACTTT CTCTCAAGACTTAGCATCAGAATCTGAGTCATCGGCCATCCTTCCTGACTTTGAACAGCTGTCATATGAA TCAGAACAAGGGACCTCAGACAGTGAAGGGTCCGGTAGAGACCTTCAGCAGAGCAACCCTGAAGAGCAGC TCCTTTCTGGCATCAGTGCTTTACATCTTCATGATTTGCCTCTAAAAGAAAGAATACGTATCAAATCTTC ATGCCCTCAGTATAATGTAGGAGCAGATGCTGGCCTGGAAAGTTTGCCCCTAAAATTGAAAGGCTCTTGT ATTGCTTACAGTAGCTCTGATGGCTCATCCAATTTTTCAAAGGATCTTACAGGGGTATATCTTCACAAAG AGTCCAGAAACTCTAAAGTTCTAGACAGCCGACTCCAAGAAAACTGCGGGGCGAATACTTCCTTACCTTA TTCTTTCAGTGACAAAGCAGTAAAGACTTCCAGTCTTCAAGTTGGGCTGCCAACTGCTGCTATACCTCAT AATCCAAGAGTTGCTGTGAAAACTACTAAGAACCTTGTCATGAAGAATAGTGTTTGCCTCGAGAGAGATT CCTCAGATGAAGACAATGCTCCAGGGTCCTGGAAATCAAAGTATACTGCTCCAGAAATGAAACACAGTTC TCAGAAGCACAGCCTAGTCCATGTCAGAGACAGTACTCACAACAAACTCAGAAATCCTAAAGTGGAATCT AAAGAAACCAAACTGTGTAATGAGTCTTTTAAAACAGCTGAAGATGAAGAAAACGGGTTCTCAGATCTAG GGAGAAGTCCTCAGAGTTTTCGACCATGTCATGACAAAGATGAGAACTCTACTGCCTCCTGGGAAAATCC TCTACCTTTACGCCAGAGGTTAAAACTCAGGTTCCAGAACACTCAAAGTGGATTTTATAATACTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_177331 |
| Insert Size | 2727 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_177331.4, NP_796305.3 |
| RefSeq Size | 3302 bp |
| RefSeq ORF | 2727 bp |
| Locus ID | 209334 |
| UniProt ID | Q8BMI4 |
| Gene Summary | Endonuclease which resolves Holliday junctions (HJs) by the introduction of symmetrically related cuts across the junction point, to produce nicked duplex products in which the nicks can be readily ligated. Four-way DNA intermediates, also known as Holliday junctions, are formed during homologous recombination and DNA repair, and their resolution is necessary for proper chromosome segregation. Cleaves HJs by a nick and counter-nick mechanism involving dual coordinated incisions that lead to the formation of ligatable nicked duplex products. Cleavage of the first strand is rate limiting, while second strand cleavage is rapid. Largely monomeric, dimerizes on the HJ and the first nick occurs upon dimerization at the junction. Efficiently cleaves both single and double HJs contained within large recombination intermediates. Exhibits a weak sequence preference for incision between two G residues that reside in a T-rich region of DNA. Has also endonuclease activity on 5'-flap and replication fork (RF) DNA substrates.[UniProtKB/Swiss-Prot Function] |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG218623 | Gen1 (tGFP-tagged) - Mouse Gen homolog 1 endonuclease (Drosophila) (Gen1), (10ug) |
CNY 7890.00 |
|
| MR218623 | Gen1 (Myc-DDK-tagged) - Mouse Gen homolog 1, endonuclease (Drosophila) (Gen1) |
CNY 6648.00 |
|
| MR218623L3 | Lenti ORF clone of Gen1 (Myc-DDK-tagged) - Mouse Gen homolog 1, endonuclease (Drosophila) (Gen1) |
CNY 9030.00 |
|
| MR218623L4 | Lenti ORF clone of Gen1 (mGFP-tagged) - Mouse Gen homolog 1, endonuclease (Drosophila) (Gen1) |
CNY 9030.00 |
