Cwc22 (NM_030560) Mouse Untagged Clone
CAT＃: MC222539
Cwc22 (untagged) - Mouse CWC22 spliceosome-associated protein homolog (S. cerevisiae) (Cwc22), transcript variant 1, (10ug)
CNY 11690.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AA684037; AI173004; AL022752; B230213M24; mKIAA1604 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC222539 representing NM_030560
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAAGTAGTGTGGCACACATGAAGTCCTCTGGTCACAACAGAAGGGAGACCCACAGTTCATACCGCC GGAGCTCCTCCCCAGAAGACAGATACACAGAGCAAGAGCGGTCCCCCCGGGATAGAGGTTACTCTGATTA CAGCCGGTCAGACTATGAGCGATCTAGAAGAGGATACTCTTACGATGACAGCATGGAATCACGAAGTAGG GACCGAGAAAAACGCAGAGAGAGAGAGAGAGATGCAGATCATCGGAAACGGTCCCGGAAATCGCCATCCC CTGACAGGAGTCCAGCCCGAGGCGGAGGGCAGAGTTCTCCTCAGGAGGAACCGACATGGAAGAAGAAGAA GGATGAGCTGGACCCGCTGCTCACCCGCACTGGGGGAGCGTATATCCCTCCTGCGAAGCTCAGGATGATG CAGGAGCAGATCACAGATAAAAGCAGCTTAGCATATCAGAGGATGAGCTGGGAGGCTCTGAAGAAATCCA TCAATGGTCTCATCAACAAGGTCAACATCTCTAATATAAGCATTATTATCCAAGAGCTCCTTCAAGAGAA CATTGTCAGAGGGAGAGGATTGCTGTCCAGATCTGTTCTGCAAGCCCAGAGTGCGTCTCCAATCTTCACT CACGTTTATGCAGCTTTGGTGGCAATAATCAACTCAAAATTCCCACAGATTGGAGAATTGATCCTCAAGA GACTAATTCTTAATTTTCGGAAAGGCTATCGAAGGAACGATAAGCAACTTTGCCTGACTGCTTCAAAATT TGTGGCACATCTTATTAACCAAAATGTGGCACATGAGGTTCTGTGCCTGGAGATGCTCACTTTGCTACTA GAGAGACCAACAGATGACAGTGTTGAAGTAGCTATCGGCTTCCTCAAAGAGTGTGGTCTCAAATTAACCC AGGTGTCACCAAGGGGAATTAATGCAATCTTTGAACGCCTTCGAAACATTCTACACGAGTCTGAGATCGA CAAAAGAGTTCAGTACATGATTGAAGTGATGTTTGCTGTACGGAAGGATGGATTCAAGGACCACCCTGTT ATTCTGGAAGGACTTGATTTAGTGGAAGAGGACGATCAGTTTACACATATGCTCCCCTTAGAGGATGACT ACAATCCAGAAGATGTTCTTAATGTTTTCAAGATGGATCCTAATTTTATGGAGAATGAAGAGAAGTACAA GGCTATTAAAAAAGAAATCCTTGACGAAGGAGACAGTGATTCAAACACAGACCAAGGAGCTGGAAGTAGT GAAGATGAGGAGGAGGAAGACGAGGAGGAGGAAGGAGAAGACGAGGAAGGAGGGCAAAAAGTAACTATTC ATGACAAAACAGAAATCAACCTAGTCTCGTTTCGCCGTACAATCTATCTTGCCATTCAGTCAAGTTTAGA TTTTGAAGAATGTGCACACAAGTTGCTGAAAATGGAGTTTGCAGAGAGCCAAACAAAAGAACTCTGCAAC ATGATACTTGATTGCTGTGCCCAACAAAGGACCTATGAAAAATTTTTTGGCTTGTTAGCTGGGCGGTTTT GTATGCTGAAAAAGGAATACATGGAATCATTTGAAAGTATATTCAAGGAACAGTACGACACCATCCATCG CTTAGAGACAAACAAATTGAGAAATGTTGCTAAAATGTTTGCTCACCTTTTATACACAGATTCACTTCCG TGGAGTGTTCTCGAATGTATAAAACTGAGTGAAGAGACCACGACATCGTCTAGTCGAATTTTTGTCAAGA TCTTCTTCCAGGAACTGTGTGAATACATGGGTCTTCCTAAACTGAATGCGAGGTTGAAGGATGAAACTCT CCAGCCATTCTTTGAAGGTCTGTTACCCAGAGACAACCCACGCAATACCCGGTTTGCCATCAACTTCTTC ACTTCCATAGGTCTCGGAGGATTGACGGATGAACTGCGAGAACATCTGAAAAATACGCCCAAGGTCATCG TAGCCCAGAAACCAGAAGCTGAACAAAAGAAACCAGCCCTGACATCTTCATCTTCAGAGTCTTCCTCGGC GTCTGATTCCTCTGACTCAGAATCTGACAGCAGCGAGAGCAGCTCAGAGTCCTCTAGTGATGCGAGCGAC TCCTCCTCCTCCTCCAGCACTCAGAGCTCTACCTCAGGTATCACGGCTCATTCAGCTAAAGGTACAAGAA AGAAGAGACAAGGGAAGGCCAGAGGCGAAGAAGTTGATAAGTTAGCCAGGGGCCACCAGGCACTTGAGCG GAGGCGAGAAGGAGGCAGGGAGGACCAAAGGCACCAGGAAGGGAGGACTGAGAGAGCAAGGTCTGAAAGA CGCAGAGCTCAGAACTCACGAGATGCAGACTGGAGAGATCCCCTAGCAAAGCACATAGACGACAGAAGCC ATGAGAATAGTCACAGCAGAGTTGGCAATGGCCGAGAACAAGGCTCACACAGAGAGCCAGAAGACAGGCA TGGCGAGCCAAAGAAGCGGAGAGAGAGAAGAGATTCTTTTTCTGAAAATGAGAAGCAAAGAAGCCGAAAT CAGGACAGTGATAATGTTAGAAGAAAAGATCGCTCCAAGTCAAGGGAGAGGAGTAGAAGGCATTCTGGTC ACAAGGGAGACGACGCCAGGTGTCAGAACAGTGCAGAGAGGCGCTGGGAAAAGCCTGGCAGACGCCCTGA ACAGTCTAGAGAGTCAAAGAGAAGTCAGGACCGGAGGAGAGAAAAGTCTCCAACAACACAGAAATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_030560 |
| Insert Size | 2727 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_030560.5, NP_085037.2 |
| RefSeq Size | 3452 bp |
| RefSeq ORF | 2727 bp |
| Locus ID | 80744 |
| UniProt ID | Q8C5N3 |
| Gene Summary | Required for pre-mRNA splicing as component of the spliceosome. Promotes exon-junction complex (EJC) assembly. Hinders EIF4A3 from non-specifically binding RNA and escorts it to the splicing machinery to promote EJC assembly on mature mRNAs. Through its role in EJC assembly, required for nonsense-mediated mRNA decay.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) differs in the 5' UTR and uses an alternate in-frame splice junction compared to variant 4. The resulting isoform (1) has the same N- and C-termini but is 1 aa shorter compared to isoform 4. Variants 1 and 5 both encode the same isoform (1). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG218485 | Cwc22 (tGFP-tagged) - Mouse CWC22 spliceosome-associated protein homolog (S. cerevisiae) (Cwc22), (10ug) |
CNY 7890.00 |
|
| MR218485 | Cwc22 (Myc-DDK-tagged) - Mouse CWC22 spliceosome-associated protein homolog (S. cerevisiae) (Cwc22), transcript variant 1 |
CNY 6648.00 |
|
| MR218485L3 | Lenti ORF clone of Cwc22 (Myc-DDK-tagged) - Mouse CWC22 spliceosome-associated protein homolog (S. cerevisiae) (Cwc22), transcript variant 1 |
CNY 9030.00 |
|
| MR218485L4 | Lenti ORF clone of Cwc22 (mGFP-tagged) - Mouse CWC22 spliceosome-associated protein homolog (S. cerevisiae) (Cwc22), transcript variant 1 |
CNY 9030.00 |
