Grik1 (NM_010348) Mouse Untagged Clone
CAT＃: MC222510
Grik1 (untagged) - Mouse glutamate receptor, ionotropic, kainate 1 (Grik1), transcript variant 2, (10ug)
CNY 11690.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | A830007B11Rik; D16Ium2; D16Ium24; D16Ium24e; Glu; GluK; GluK1; GluK5; Glur; Glur-5; Glur5; Glurbe; Glurbeta1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC222510 representing NM_010348
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCGCGGCACAGTCCTTATCCAACCCGGGCTCTGGACCAGGGACACCAGCTGGACACTCCTCTATT TCCTGTGCTACATCCTTCCTCAGACCTCCCCTCAAGTGCTCAGGATCGGAGGGATTTTTGAAACTGTGGA AAACGAACCTGTTAATGTTGAAGAATTAGCTTTCAAGTTTGCAGTCACCAGTATTAACCGAAACCGAACC TTGATGCCCAATACCACATTAACCTATGACATCCAGAGAATTAATCTTTTTGATAGTTTTGAAGCCTCCC GAAGAGCATGCGACCAACTGGCTCTTGGGGTGGCCGCCCTCTTCGGTCCTTCCCACAGCTCCTCCGTCAG TGCTGTACAGTCTATTTGCAATGCTCTGGAAGTTCCACACATTCAGACTCGCTGGAAACACCCTTCTGTG GACAACAGAGACTTATTTTACATCAACCTCTACCCAGATTATGCAGCTATCAGCAGGGCAGTCCTGGATC TGGTCCTCTATTACAACTGGAAAACAGTGACGGTGGTGTACGAAGACAGCACAGGTCTAATTCGTCTGCA AGAGCTCATCAAAGCTCCCTCCAGATACAACATCAAAATCAAAATCCGCCAGCTTCCCTCTGGCAATAAG GATGCCAAACCTCTGCTCAAGGAGATGAAGAAAGGCAAGGAGTTCTATGTGATATTTGATTGTTCGCACG AGACGGCTGCTGAAATTCTTAAGCAGATTTTGTTCATGGGCATGATGACTGAATACTATCACTACTTCTT CACAACCCTGGACTTGTTTGCTTTGGATCTGGAACTCTACAGGTACAGTGGTGTAAATATGACTGGATTT CGGTTGCTGAATATTGACAACCCTCACGTGTCATCCATCATTGAGAAGTGGTCCATGGAGAGATTGCAGG CCCCACCCAGACCTGAGACTGGTCTCCTGGACGGCGTGATGACAACTGAAGCAGCTCTGATGTACGATGC TGTGTACATGGTAGCCATCGCCTCTCACCGTGCCTCTCAGCTGACCGTCAGTTCCCTGCAGTGCCATCGA CATAAGCCATGGCGCCTAGGACCCAGATTTATGAACCTCATCAAAGAGGCGCGGTGGGATGGCTTGACGG GGCGGATCACCTTCAATAAGACGGATGGCTTGAGAAAGGATTTTGACCTGGACATTATCAGTCTCAAAGA GGAAGGAACTGAAAAGATTGGGATTTGGAACTCCAACAGTGGGCTGAACATGACGGATGGCAACAGAGAC AGGTCCAACAATATCACAGATTCGCTGGCTAACCGAACGCTCATTGTCACCACTATTCTGGAAGAGCCCT ACGTGATGTACAGGAAATCCGATAAACCACTGTACGGAAATGACAGATTTGAAGGATATTGCCTGGATCT GCTGAAAGAACTGTCAAATATCCTAGGTTTCCTTTATGATGTTAAACTGGTTCCTGACGGCAAATATGGA GCCCAGAATGACAAAGGGGAGTGGAACGGGATGGTTAAGGAACTCATCGACCACAGAGCTGACCTAGCAG TGGCCCCTCTCACCATCACGTATGTACGGGAGAAAGTCATTGACTTCTCCAAGCCTTTCATGACTCTGGG CATTAGCATCCTTTACCGGAAGCCCAATGGAACCAACCCCGGCGTCTTCTCCTTCCTCAACCCCCTGTCT CCAGACATTTGGATGTATGTGCTCCTCGCTTGCCTAGGAGTCAGTTGTGTGCTTTTTGTGATTGCAAGGT TCACACCCTACGAGTGGTATAACCCCCACCCGTGCAACCCTGACTCAGACGTGGTGGAAAACAATTTCAC TTTGCTAAATAGTTTCTGGTTTGGCGTTGGAGCTCTCATGCGGCAAGGATCGGAGCTGATGCCCAAGGCT CTATCGACCAGAATAGTTGGAGGAATATGGTGGTTTTTCACCCTAATCATCATCTCATCCTACACTGCCA ACCTGGCTGCCTTCTTGACAGTAGAAAGGATGGAATCCCCCATCGATTCCGCAGACGACCTGGCCAAACA AACCAAGATAGAATACGGGGCAGTCAGAGATGGCTCGACAATGACCTTCTTCAAGAAATCAAAAATCTCC ACGTATGAGAAAATGTGGGCTTTCATGAGCAGTAGACAGCAGAGCGCCCTGGTTAAAAACAGCGATGAGG GGATCCAAAGGGTGCTTACCACCGACTACGCCCTGCTGATGGAGTCCACCAGCATTGAGTATGTGACACA GAGGAACTGCAACCTCACTCAGATCGGGGGCCTCATAGACTCCAAAGGCTATGGAGTGGGGACACCTATC GGCTCCCCTTACCGGGATAAAATTACAATTGCTATTCTTCAACTACAAGAAGAAGGGAAGCTTCATATGA TGAAAGAGAAATGGTGGAGGGGAAATGGCTGCCCTGAAGAAGACAGTAAAGAAGCCAGTGCTCTAGGAGT GGAAAATATCGGGGGTATCTTCATTGTTCTGGCTGCAGGACTCGTCCTTTCTGTGTTTGTAGCCATTGGA GAATTCATATACAAATCACGGAAGAACAATGACATTGAGCAGTGTCTCTCTTTCAATGCCATCATGGAAG AGCTGGGAATCTCACTCAAGAATCAGAAAAAATTAAAGAAAAAGTCAAGAACTAAGGGCAAATCTTCTTT CACAAGTATCCTTACTTGTCATCAGAGACGAACTCAGAGAAAAGAGACTGTGGCGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_010348 |
| Insert Size | 2718 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_010348.3, NP_034478.1 |
| RefSeq Size | 3573 bp |
| RefSeq ORF | 2718 bp |
| Locus ID | 14805 |
| Gene Summary | Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. The subunit encoded by this gene is subject to RNA editing (CAG->CGG; Q->R) within the second transmembrane domain, which is thought to alter the properties of ion flow. Alternative splicing, resulting in transcript variants encoding different isoforms, has been noted for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) is missing an in-frame coding exon at the 3' end compared to transcript variant 1, resulting in a shorter isoform (b) lacking a 29 aa segment compared to isoform a. RNA editing (CAG->CGG) changes Gln621Arg. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG222519 | Grik1 (tGFP-tagged) - Mouse glutamate receptor ionotropic kainate 1 (Grik1) transcript variant 2, (10ug) |
CNY 8232.00 |
|
| MR222519 | Grik1 (Myc-DDK-tagged) - Mouse glutamate receptor, ionotropic, kainate 1 (Grik1), transcript variant 2 |
CNY 6632.00 |
|
| MR222519L3 | Lenti ORF clone of Grik1 (Myc-DDK-tagged) - Mouse glutamate receptor, ionotropic, kainate 1 (Grik1), transcript variant 2 |
CNY 9790.00 |
|
| MR222519L4 | Lenti ORF clone of Grik1 (mGFP-tagged) - Mouse glutamate receptor, ionotropic, kainate 1 (Grik1), transcript variant 2 |
CNY 9790.00 |
