Zfp473 (NM_178734) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	D030014N22Rik; zfp-100; Znf473
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC222446 representing NM_178734 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAGGAAGGAGGACGACCTTAAAGGAGGTTGCTGGAATCAGCCAGCTGTGGCTGAGGAGTTTGAGA CCCTCAAGGATTTAGCCATGGATTTCACCGTGGAAGACTGGAAAGACCTGGAGTCAGAGTGGGACCAGAG AGACCTGTTCTGGGATGTGACGCTGAACCACTACCAGGACATGTTCTCATTTACAGATACTTCGCAACCC AGCCTGACTTCCCAGCCAGATGTCAGGGAAGAGCTGGAAGCCACCAGTACTGAAGTGCCTGAGACCAAGA GCTCTCCTTTGCAGAGTGGTTTCGTAGAAGAAGACTTCTCCCAGATTATGGAGATATTTTCCAACGGGCA ACTCAACTTTGAAGCCTGTATAGGTGAGGATTGGCTAAATAGTTTTCTCGGAGATCCAGAAAGTCTTCCA AGACCTGACATCAGCGACAAGGAAAGCCCCGCGGATCATCAGAGTCCTGAGTCCAAGAGCGGCCTCAGTC CTGGGCCTCCCCTTTGCACAAGAGAGGATGCTGTGATGTCTGCTAGTCCTGAAAAGACCCTCACTCCAGT GATACTGAAGGAATCCAGGAGTGACCTGAGCCAGGAGGACTCTGTCCAGGGACACGAGAAGCCATATAAG TGTAGTGAGTGTGGGGAAAGCTTCAGCCAGAGTCACCACCTCATCCAGCACTGGGTTCTCCACACCTCAG GGGAGCCTCCTATATGGCGAGAGCAGCAGCGAGGTCTCAGCCAGGGTGCTCACTTTCCCATGTGTCCAGG GACTCCAGCAAGCTATGAATCCTACACGTGTCAGGAGTGTGGGAAAAGGTTTAGTCAGAATGTGTACCTC CAGTGGCATCAGAAAATCCACACTGGAGAAAAACTTTGTAAAACTCAAAGTGACAGCAACCTAGAAGGGC TCTCTAGGAGTCCGAGTGTCGAGCCAGGAAAGCAGCGGCTGAGTAAAGACACTGACTCAGCAAAACCGAG TACCATTCACGGTCAGGACCAGGAAAAACCACCTACTGGTGAAAGTCGTGACCAAGAAAACCTGCATGAG AGCCAGCCTGGTGACAGGCCATCCGTTCTTCATCCAAAGCCTCTTAGGCATCAGAAGACTCCTACAAACG CTAAGTGCTTCAGATGTAAGAAGTGTGGGGAGACATTCAGTGGGGCCTTTCATCTAGCTAAACACCAGAG AGCCCATGCTCAGAGACTCTACAAGTGCGCTTCGTGCCCTGCCGTCTTCAACCTAAGCAAGCATTGCTTC CAGCATCGCAAGTCACATTTCCCCAGCGCGGCCTGCGAGTGTCAGGGATGCAGAAAGAGCTTTAATTGGC GGTCATCACTCATTAAGCACCAGGCTATTCACAAAGGAGAGAAGCCGTACAAGTGTGATGAATGTGGTAA AGCTTTCAACCACTCCTCCACCCTGAAGATCCACCAGAGGATCCACAGTGGACAGAAGCCTCACAAATGC AGTGAGTGCGGGAAAGCCTTCTGTCGGAGAACAGATCTTACTGAGCATCAGCGAGTCCACTCGGGCTTCA GACCCCATCAGTGTCCCGTGTGTGCCAGGACTTTTAACCGACCTTCACACTTAGTTCGACACCGGCTGAG ACATGCTGAAGAAAGGCACTTTGGCTGTGCTAAATGCAAGGAGACCTTCATCTATAAAGAACAGCTAGAG CGGCACAATAAAATCCACACCATCGAGGGCTTGTATGAGTGTAAGCAGTGTGGAGAACACTTCATTTGCA GGTCAACTCTGAATTGCCACCTGAGCATACACATTAGAGAAAATACAAGTGAGAAAGTTGTGGGTCAGAA CTCACAGCACACTGAAAAATGCTTTAAGAATACCAAGTGTAGAAAAGCCCCTAACCACAGCAGATACCTA GGGCAGCATGAGAAGATTCATGCCCAGGTGACATCCGGTGAGTGTGACCCATGTGGGGAAACCTATGACC AGAGTGTGCAGCCCATTTGCCATCAGAGCATCTGTGCTGGTGTGAAACCAAGTGAATGCGCTGAGCCTGA GAAATGCACGCGAAACACCTCTGCCAGTGAGCACCATCCTTCCCAAAGGGAACCATCCTTCAAGTGTGAC ATATACAACAGGGCCTTCAAACAGCGTGCCCACCTTTCAAAACATCAGTTGATTCACATCACTGAGAAGC CCTTTAAATGCAACGAATGTGACAGAGCCTTCAAGCAGAGTAACTACCTCATTCAGCATCAGAAGACTCA CACTGCAGAGAAGCACTTTGAATGTAGCGAGTGTGGGAAAACATTTCATCAGCGATCATGCCTTTCTAAG CACCAGAAAATCCACTCAGGGGAGAAGCCCTTTAAGTGTGGTGACTGTGGGAAAGCCTTCATCTCTGGTG CCCAGCTCATCCGACACCAGAGAATTCACACTGGGGAGAAGCCGTACGTCTGCCAGGAATGTGGGAAAAC CTTCAGCCAGAGCTCGTGCCTTACCCTTCACCTTAGAATTCACACTGGGGAGAAGCCGTACACATGCGGC ACGTGCGGGAAAGCCTTTGCTCAGAGAGCAAACCAGAGGAAGCACGAGAGAATTCACACTGGGGAGAAGC CCTATGCCTGCGGGCTGTGTGGGAAGGCCTTCGGCCTCCGCACCCATCTCCAGCAACACCAGAGAATTCA CACCAAGGCCAAACCATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_178734
Insert Size	2679 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_178734.4, NP_848849.2
RefSeq Size	3809 bp
RefSeq ORF	2679 bp
Locus ID	243963
UniProt ID	Q8BI67
Gene Summary	Involved in histone 3'-end pre-mRNA processing by associating with U7 snRNP and interacting with SLBP/pre-mRNA complex. Increases histone 3'-end pre-mRNA processing but has no effect on U7 snRNP levels, when overexpressed. Required for cell cycle progression from G1 to S phases (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same isoform (1).

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