Mical3 (NM_153396) Mouse Untagged Clone
CAT＃: MC222284
Mical3 (untagged) - Mouse microtubule associated monoxygenase, calponin and LIM domain containing 3 (Mical3), (10ug)
CNY 11120.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | C130040D16Rik; Mical-3; mKIAA1364 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC222284 representing NM_153396
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGAGAGGAAGCAGGAGACAACGAACCAAGCTCACGTCCTCTTTGACCGCTTTGTCCAGGCCACCA CCTGCAAGGGGACCCTCAGGGCCTTCCAAGAGCTCTGTGACCACCTGGAGCTGAAGCCCAAGGACTACCG GTCCTTCTACCACAAGCTCAAGTCCAAGCTCAACTACTGGAAGGCCAAAGCCCTCTGGGCCAAATTGGAC AAGCGGGGCAGTCACAAAGACTACAAAAAGGGCAAAGCCTGCACCAACACAAAGTGTCTCATCATTGGAG CTGGCCCCTGTGGGCTGCGCACAGCCATCGACTTGTCCTTGTTGGGAGCCAAGGTGGTTGTTATTGAAAA GCGAGATGCCTTCTCCCGCAACAATGTCTTACATCTCTGGCCCTTCACCATACACGATCTCCGAGGCCTG GGTGCCAAGAAATTCTACGGCAAGTTCTGTGCCGGAGCCATCGACCATATCAGTATCCGTCAGCTCCAGC TAATCCTCTTGAAGGTAGCCTTGATCTTGGGCATTGAGATCCATGTCAATGTGGAATTCCAAGGACTTGT TCAGCCTCCCGAGGACCAAGAGAATGAACGGATAGGATGGCGAGCATTGGTGCACCCCAAAACTCATCCT GTATCAGAATATGAATTTGAAGTTATCATCGGTGGAGATGGGCGTAGGAACACCTTGGAAGGATTTCGTC GAAAAGAATTTCGTGGCAAGCTAGCCATTGCCATTACAGCAAATTTTATCAACCGCAACACAACCGCAGA GGCCAAAGTGGAGGAGATTAGTGGCGTGGCTTTTATATTCAACCAAAAATTTTTCCAGGAGCTGAGAGAA GCCACAGGTATTGACTTAGAGAACATCGTCTACTACAAAGATGACACACACTACTTTGTCATGACGGCCA AAAAGCAGAGTTTGCTGGACAAGGGGGTGATCCTGCATGACTACACAGACACGGAGCTGTTGCTTTCCCG GGAGAACGTGGACCAGGAGGCCCTGCTGAACTATGCCCGGGAGGCCGCAGACTTCTCTACCCAGCAGCAG CTGCCTTCTCTGGATTTCGCCATCAATCACTACGGGCAGCCAGACGTGGCCATGTTTGACTTTACCTGCA TGTACGCCTCTGAAAATGCCGCCTTGGTCCGGGAACAGAACGGACACCAGTTACTAGTAGCTTTGGTTGG AGACAGCCTCCTGGAGCCTTTCTGGCCAATGGGGACAGGAATAGCCCGGGGCTTTCTGGCTGCTATGGAC TCTGCCTGGATGGTACGAAGCTGGTCTCTGGGCACCAGCCCCTTGGAAGTCCTGGCAGAAAGGGAAAGCA TTTATAGGTTGCTGCCTCAGACCACCCCTGAGAACGTGAGCAAAAACTTCAGCCAATATAGCATCGACCC TGTCACTAGGTATCCCAATATCAACATCAACTTCCTCAGGCCAAGCCAGGTGCGCCATTTATACGATAGC GGAGAAACAAAAGATATCCACCTGGAAATGGAGAACATGGTGAATCCCCGAACCACTCCCAAGCTGACTC GCAACGAGTCTGTAGCTCGTTCAAGCAAACTGCTGGGGTGGTGCCAAAGGCAGACAGAAGGCTATTCAGG AGTCAATGTGACAGATCTCACTATGTCTTGGAAAAGTGGCCTGGCCCTGTGTGCGATCATCCACAGATAC CGCCCCGATCTAATAGACTTTGATTCTTTGGATGAGCAAAACGTGGAGAAGAATAATCAGCTGGCCTTTG ACATCGCTGAGAAGGAACTGGGTATCTCTCCCATCATGACGGGCAAGGAGATGGCTTCGGTCGGGGAGCC AGACAAGCTGTCCATGGTGATGTACCTCACGCAGTTCTACGAGATGTTCAAGGACTCGCTCTCCTCCAGC GACACCCTCGACCTGAATGCAGAGGAGAAAGCCGTCCTGATAGCCAGCACCAAATCCCCCATCTCCTTCC TGAGCAAACTCGGACAGACCATCTCTCGGAAGCGTTCGCCCAAGGATAAAAAAGAAAAGGACTCAGATGG GGCTGGAAAGAGGAGAAAAACCAGTCAGTCAGAGGAGGAGGAGCCTCCCCGGAGCTACAAAGGAGAAAGA CCGACCCTGGTGAGCACCCTGACGGACAGACGGATGGATGCTGCCGTTGGGAACCAGAACAAAGTGAAGT ACATGGCGACCCAGCTGCTGGCCAAGTTTGAAGAGAACGCACCTGCTCAGTCCACTGGTGTGAGGAGACA GGGCTCCATAAAGAAAGAGTTCCCGCAGAACTTGGGGGGCAGCGACACGTGCTATTTCTGCCAAAAGCGG GTCTACGTGATGGAGAGGCTGAGCGCCGAGGGCAAGTTCTTCCATCGAAGCTGCTTCAAGTGTGAGTACT GTGCCACCACGCTGCGCCTATCAGCCTACGCCTACGACATCGAGGATGAGTTCTCTCCAAACTTCTGGAC ATCAGCCAGCTACCATGTTCCAGTTGCCCTTCCTGCTACGGTCATGCCTATGTGCCTTCTGTACCATCCA TCTCAAGTGCTCGTCTGCCTCGAGGGAGGCCCAGCATTCATGTCACCAGTGTTATTCAATGACACCAACA GTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_153396 |
| Insert Size | 2595 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_153396.3, NP_700445.2 |
| RefSeq Size | 2929 bp |
| RefSeq ORF | 2595 bp |
| Locus ID | 194401 |
| UniProt ID | Q8CJ19 |
| Gene Summary | Monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin to form methionine-sulfoxide, resulting in actin filament disassembly and preventing repolymerization. In the absence of actin, it also functions as a NADPH oxidase producing H(2)O(2). Seems to act as Rab effector protein and play a role in vesicle trafficking. Involved in exocytic vesicles tethering and fusion: the monooxygenase activity is required for this process and implicates RAB8A associated with exocytotic vesicles. Required for cytokinesis. Contributes to stabilization and/or maturation of the intercellular bridge independently of its monooxygenase activity. Promotes recruitment of Rab8 and ERC1 to the intercellular bridge, and together these proteins are proposed to function in timely abscission.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR, lacks several 3' exons and includes an alternate 3' exon. The encoded isoform (2) has a distinct and shorter C-terminus compared to isoform 1. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG220854 | Mical3 (tGFP-tagged) - Mouse microtubule associated monoxygenase calponin and LIM domain containing 3 (Mical3), (10ug) |
CNY 7890.00 |
|
| MR220854 | Mical3 (Myc-DDK-tagged) - Mouse microtubule associated monoxygenase, calponin and LIM domain containing 3 (Mical3) |
CNY 6336.00 |
|
| MR220854L3 | Lenti ORF clone of Mical3 (Myc-DDK-tagged) - Mouse microtubule associated monoxygenase, calponin and LIM domain containing 3 (Mical3) |
CNY 9030.00 |
|
| MR220854L4 | Lenti ORF clone of Mical3 (mGFP-tagged) - Mouse microtubule associated monoxygenase, calponin and LIM domain containing 3 (Mical3) |
CNY 9030.00 |
