Stt3b (NM_024222) Mouse Untagged Clone
CAT＃: MC221873
Stt3b (untagged) - Mouse STT3, subunit of the oligosaccharyltransferase complex, homolog B (S. cerevisiae) (Stt3b), (10ug)
CNY 10550.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 1300006C19Rik; Simp |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC221873 representing NM_024222
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGCCCTCGGCCCCGGAGAGCAAGCACAAGTCGTCCCTCAACTCGTCCCCGTGGAGCGGCCTCA TGGCTCTGGGGAACAGCCGCCACGGGCACCATGGGCCCGGAACCCAGAGCGCGTCCAGGGCGGCGGCGCC GAAGCCGGGGCCCCCCGCGGGGCTGTCCGGGGGCTTGTCGCAGCCGGCCGGGTGGCAGTCGTTGCTCTCC TTCACCATCCTCTTCCTGGCCTGGCTGGCCGGCTTCAGCTCGCGCCTCTTCGCCGTCATCCGCTTCGAGA GCATCATCCACGAGTTCGACCCGTGGTTTAACTATAGATCAACACATCATCTTGCATCTCATGGATTCTA TGAGTTTCTAAATTGGTTTGATGAAAGAGCATGGTACCCACTGGGAAGAATAGTGGGTGGCACCGTTTAC CCAGGGTTGATGATAACAGCTGGCCTTATTCATTGGATTTTAAATACATTGAACATAACAGTTCACATAA GAGATGTGTGTGTATTCCTTGCACCAACTTTTAGCGGCCTTACATCCATATCTACGTTCCTGCTAACTAG AGAACTGTGGAACCAAGGAGCAGGACTTCTAGCTGCTTGCTTCATTGCTATCGTACCAGGGTACATATCT CGGTCAGTGGCGGGATCCTTTGATAATGAAGGCATTGCCATTTTTGCGCTTCAGTTCACTTACTACTTAT GGGTAAAGTCTGTGAAGACCGGGTCTGTGTTCTGGACAATGTGCTGCTGCTTGTCATATTTCTACATGGT CTCTGCGTGGGGAGGTTATGTGTTCATCATCAACCTCATCCCTCTCCATGTGTTTGTGTTGCTGCTGATG CAGAGGTACAGCAAGAGAGTCTACATAGCATATAGCACTTTCTACATTGTGGGTTTAATATTATCCATGC AGATACCTTTTGTGGGATTTCAGCCAATCAGAACAAGCGAGCACATGGCAGCTGCAGGTGTCTTTGCGCT GCTGCAAGCTTACGCTTTTTTGCAGTATCTGAGAGACCGGTTGACAAAACAGGAGTTCCAGACCCTTTTC TTTTTGGGTGTCTCACTAGCTGCAGGCGCTGTGTTCCTTAGTGTCATCTATCTGACATACACAGGTTATA TTGCACCATGGAGTGGCAGGTTTTATTCACTATGGGATACTGGGTATGCAAAAATACACATTCCAATTAT TGCATCAGTGTCTGAACATCAGCCTACGACATGGGTGTCTTTCTTCTTTGATCTACATATTCTTGTATGT ACCTTCCCAGCAGGCCTATGGTTCTGCATCAAAAATATCAACGATGAAAGAGTATTTGTCGCTCTGTATG CGATCAGTGCTGTCTACTTTGCCGGAGTGATGGTGCGGCTGATGCTGACTCTGACCCCGGTCGTCTGCAT GCTGTCGGCCATCGCCTTCTCCAATGTTTTTGAGCACTATTTGGGGGATGACATGAAAAGGGAAAACCCA CCTGTGGAGGACAGCAGTGATGAGGATGACAAAAGAAACCCAGGAAACTTGTATGACAAGGCAGGTAAAG TGAGGAAGCATGTGACAGAGCAAGAGAAACCTGAAGAGGGCTTGGGCCCCAACATCAAAAGCATTGTGAC CATGCTGATGCTCATGCTCCTGATGATGTTCGCGGTCCACTGCACGTGGGTCACAAGCAACGCCTACTCC AGTCCAAGTGTGGTCCTTGCCTCCTACAATCATGATGGTACCAGGAATATATTAGATGATTTTAGAGAAG CGTACTTTTGGCTGAGACAAAACACGGATGAACACGCCCGGGTCATGTCGTGGTGGGACTACGGCTATCA GATTGCTGGCATGGCCAACAGGACCACTCTGGTGGATAACAACACCTGGAACAACAGCCACATCGCACTG GTCGGAAAAGCTATGTCTTCCAATGAAACGGCCGCCTATAAAATCATGAGGTCCCTTGATGTCGATTATG TGTTGGTTATTTTCGGAGGAGTGATTGGCTATTCCGGGGACGATATCAACAAGTTCCTCTGGATGGTCAG GATAGCTGAAGGGGAGCATCCCAAAGACATCCGGGAAGGTGACTATTTCACCCAGCAGGGAGAGTTCCGA GTAGACAAAGCTGGGTCTCCTACTCTGTTAAACTGCCTTATGTATAAAATGTCATACTACAGATTTGGAG AAATGCAGCTAGATTTTCGCACTCCCCCAGGCTTTGACCGAACACGTAATGCTGAGATTGGAAATAAAGA CATTAAATTCAAGCATTTGGAGGAAGCTTTTACATCAGAGCACTGGCTTGTCAGGATATATAAAGTGAAA GCACCTGACAACAGGGAGACACTAGGTCACAAACCTCGAGTCACCAACATCGTCCCCAAACAGAAGTATT TGTCAAAGAAGACTACTAAAAGGAAGCGTGGCTACGTTAAAAATAAGCTAGTGTTTAAGAAAGGCAAGAA GACCTCTAAGAAGACTGTTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_024222 |
| Insert Size | 2472 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_024222.2, NP_077184.2 |
| RefSeq Size | 4236 bp |
| RefSeq ORF | 2472 bp |
| Locus ID | 68292 |
| UniProt ID | Q3TDQ1 |
| Gene Summary | Catalytic subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity. This subunit contains the active site and the acceptor peptide and donor lipid-linked oligosaccharide (LLO) binding pockets (By similarity). STT3B is present in a small subset of OST complexes and mediates both cotranslational and post-translational N-glycosylation of target proteins: STT3B-containing complexes are required for efficient post-translational glycosylation and while they are less competent than STT3A-containing complexes for cotranslational glycosylation, they have the ability to mediate glycosylation of some nascent sites that are not accessible for STT3A. STT3B-containing complexes also act post-translationally and mediate modification of skipped glycosylation sites in unfolded proteins. Plays a role in ER-associated degradation (ERAD) pathway that mediates ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins by mediating N-glycosylation of unfolded proteins, which are then recognized by the ERAD pathway and targeted for degradation (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG210829 | Stt3b (tGFP-tagged) - Mouse STT3, subunit of the oligosaccharyltransferase complex, homolog B (S. cerevisiae) (Stt3b) |
CNY 7980.00 |
|
| MR210829 | Stt3b (Myc-DDK-tagged) - Mouse STT3, subunit of the oligosaccharyltransferase complex, homolog B (S. cerevisiae) (Stt3b) |
CNY 6032.00 |
|
| MR210829L3 | Lenti ORF clone of Stt3b (Myc-DDK-tagged) - Mouse STT3, subunit of the oligosaccharyltransferase complex, homolog B (S. cerevisiae) (Stt3b) |
CNY 9120.00 |
|
| MR210829L4 | Lenti ORF clone of Stt3b (mGFP-tagged) - Mouse STT3, subunit of the oligosaccharyltransferase complex, homolog B (S. cerevisiae) (Stt3b) |
CNY 9120.00 |
