Rgl1 (NM_016846) Mouse Untagged Clone
CAT＃: MC221430
Rgl1 (untagged) - Mouse ral guanine nucleotide dissociation stimulator,-like 1 (Rgl1), (10ug)
CNY 9880.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | mKIAA0959; R; Rgl |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC221430 representing NM_016846
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAATTACTTTGGCAAGCTAAAATGAGCTCGATTCAGGACTGGGGTGAAGAGGTAGAGGAAGGAGCTG TTTACCATGTCACCCTCAAAAGAGTCCAGATTCAACAGGCGGCCAATAAAGGAGCGAGATGGCTAGGGGT TGAAGGGGACCAGCTGCCTCCAGGACACACAGTCAGTCAGTACGAGACCTGCAAGATCAGGACCATCAAA GCTGGTACGCTGGAGAAGCTTGTGGAGAACCTGCTGACGGCTTTTGGGGACAATGACTTTACCTACATCA GCATCTTTTTGTCGACATACAGAGGCTTTGCCTCGACTAAGGAAGTGCTGGAGCTGCTGCTGGACAGGTA TGGAAACCTGACAGGCCCAAACTGTGAAGACGATGGAAGCCAAAGTTCACCCGAGTCCAAGGCCGTGATC CGGAATGCCATTGCTTCCATCCTGAGGGCCTGGCTTGACCAGTGTGCGGAAGACTTCCGGGAGCCCCCTC ACTTCCCTTGCCTTCAGAAGCTGCTGGAGTACCTCAAACAGATGATGCCTGGCTCTGACCCAGAGAGGAG AGCACAGAACCTTCTTGAACAGTTTCAAAAGCAGGACGTGGATTCCGACAATGGACTTCTCAACACCAGC TCCTTCAGCCTGGAAGAGGAAGAGGAACTGGAGAGCGGAGGGTCAGCAGAATTCACGAACTTCTCAGAAG ATCTCGTGGCAGAACAGCTGACCTACATGGACGCACAACTATTCAAGAAGGTAGTGCCTCACCATTGCCT GGGCTGTATTTGGTCTCAGCGGGATAAAAAGGAAAACAAGCATTTGGCTCCTACGATCCGTGCCACCATC TCTCAGTTTAATACGCTCACCAAGTGTGTTGTCAGCACCGTCCTGGGGAGCAAGGAACTCAAAACTCAGC AGCGAGCCAGAGTCATCGAGAAGTGGATCAACATTGCTCACGAATGTAGAATCCTGAAGAATTTTTCCTC CTTGAGGGCCATCGTTTCCGCACTGCAGTCTAATTCCATCTATCGGTTGAAAAAGGCTTGGGCTGCTGTC CCGAAGGACAGAATGCTGATGTTTGAAGAACTTTCAGATATCTTCTCTGATCACAATAACCATCTAACCA GTCGGGAGCTACTAATGAAGGAAGGAACTTCAAAATTTGCAAACCTGGACAGCAGCGTGAAAGAAAACCA GAAGCGGACCCAGAGGCGCCTGCAACTGCAGAAGGATATGGGTGTGATGCAGGGTACCGTGCCTTACCTG GGCACCTTCCTGACTGACCTGACCATGCTGGACACTGCCCTGCAGGACTACATTGAGGGTGGACTGATCA ACTTCGAGAAAAGAAGAAGGGAATTTGAAGTCATTGCCCAGATAAAGCTCCTACAGTCTGCTTGCAACAG CTACTGCATGGGCCCAGACCAGAAGTTTATCCAGTGGTTCCAGAGGCAGCAGCTTCTATCAGAGGAGGAA AGCTACGCCCTCTCGTGTGAGATTGAAGCCGCTGCCGACGCCAACACCACTTCCCCTAAGCCTCGGAAAA GCATGGTGAAGAGGCTGAGCCTGCTATTTCTGGGGTCTGACATCATCCCCGGGAGCACTCCCACCAAAGA GCAGCCCAAGTCCGCAGCCAGTGGGAGCTCTGGGGAGAGTATGGACTCAGTCAGTGTGTCGTCCTGTGAA TCAAACCACTCCGAGGCTGAGGAGGGCCCCGTCACACCCATGGACACACCAGATGAGCCCCAAAAGAAGC TCTCTGAATCCTCCTCTTCCTGTTCCTCCATCCATTCCATGGACACGAATTCCTCAGGGATGTCGTCCCT AATCAACCCCCTGTCCTCCCCTCCAACGTGCAACAACAATCCTAAAATCCACAAGCGCTCCGTCTCCGTG ACATCCATTACCTCCACAGTACTGCCTCCTGTTTACAATCAGCAGAACGAAGACACCTGCATCATCCGCA TCAGTGTAGAAGACAACAATGGCAACATGTACAAGAGCATCATGCTGACAAGCCAGGATAAGACCCCCGC TGTGATCCAGAGAGCGATGTCGAAGCACAACCTGGAGTCGGACCCCGCCGAGGAGTATGAGCTGGTGCAG GTCATCTCGGAGGACAAAGAACTAGTGATCCCGGACTCTGCAAACGTCTTTTACGCCATGAATAGCCAAG TGAACTTTGATTTCATTTTACGCAAAAAGAACTCGGTGGAGGAGCAGGTGAAGTTGCGCAGTCGGACCAG CCTGACTTTGCCCAGGACAGCTAAGCGGGGCTGCTGGAGTAACAGGCACAGCAAGATCACCCTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_016846 |
| Insert Size | 2307 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_016846.3, NP_058542.2 |
| RefSeq Size | 3887 bp |
| RefSeq ORF | 2307 bp |
| Locus ID | 19731 |
| UniProt ID | Q60695 |
| Gene Summary | This gene encodes a member of the Ras-like (Ral) -selective guanine nucleotide exchange factor (RalGEF) family of small GTPase activators which function both as downstream effectors of activated Ras GTPase and as regulators of certain Ral GTPases in the RalGEF - Ral GTPase signaling pathway. The encoded protein, like other RalGEFs, has an N-terminal ras exchanger motif domain, a catalytic CDC25 homology domain, and a C-terminal ras binding domain that stimulates guanine nucleotide exchange when bound to a Ral GTPase. RalGEF family members bridge the Ras and Ral signaling pathways and are thought to play a role in oncogenic transformation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016] Transcript Variant: This variant (4) represents use of an alternate promoter and therefore differs in the 5' UTR and 5' coding region, compared to variant 1. These differences cause translation initiation at an alternate start codon and result in an isoform (3) with a shorter and distinct N-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. COMPLETENESS: complete on the 3' end. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR210552 | Rgl1 (Myc-DDK-tagged) - Mouse ral guanine nucleotide dissociation stimulator,-like 1 (Rgl1) |
CNY 5624.00 |
|
| MR210552L3 | Lenti ORF clone of Rgl1 (Myc-DDK-tagged) - Mouse ral guanine nucleotide dissociation stimulator,-like 1 (Rgl1) |
CNY 8550.00 |
|
| MR210552L4 | Lenti ORF clone of Rgl1 (mGFP-tagged) - Mouse ral guanine nucleotide dissociation stimulator,-like 1 (Rgl1) |
CNY 8550.00 |
