Fchsd2 (NM_199012) Mouse Untagged Clone
CAT＃: MC221418
Fchsd2 (untagged) - Mouse FCH and double SH3 domains 2 (Fchsd2), transcript variant 1, (10ug)
CNY 9880.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | BC034086; mKIAA0769; R74866; Sh3md3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC221418 representing NM_199012
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGCCGCCGCCGAGGAAGGTGAAAGTTACACAAGAGCTGAGAAACATTCAAGGTGAGCAGATGACAA AACTTCAAGCCAAACATCAGGCAGAATGTGACTTACTTGAAGACATGAGGACTTTCAGTCAGAAGAAGGC TGCTATTGAAAGAGAGTATGCACAGGGTATCCAGAAGTTGGCTAGTCAATACCTGAAGAGAGATTGGCCT GGAATAAAAACTGATGATCGGAATGATTACAGGAGTATGTATCCTGTTTGGAAATCTTTTCTCGAGGGAA CAATGCAGGTAGCCCAGTCTCGGATCAATATATGTGAAAACTATAAAAACTTCATTTCTGAACCTGCAAG AGCAGTAAGAAGCTTAAAAGAACAGCAACTAAAACGGTGTGTGGATCAGTTGACAAAGATCCAAACAGAA TTACAGGAAACTGTGAAAGATTTAGTGAAAGGCAAAAAGAAGTACTTTGAGACTGAACAGATGGCTCATG CAGTAAGAGAGAAAGCTGACATCGAAGCAAAGTCTAAACTTAGTCTTTTTCAATCAAGAATCAGTTTACA GAAAGCAAGTGTGAAGTTAAAAGCCCGGCGATCTGAATGTAATACCAAAGCTACCCATGCAAGGAATGAT TACCTTCTGACGCTAGCAGCAGCTAACGCACATCAGGACCGCTACTATCAAACAGACTTAGTTAACATCA TGAAGGCTCTTGATGGAAACGTGTATGACCACCTCAAGGATTATTTAATAGCCTTCAGCCGGACCGAGCT AGAAACTTGCCAAGCTATACAGAATACATTCCAGTTTTTACTAGAAAACTCCAGCAAGGTGGTACGGGAC TATAACCTTCAGCTGTTTCTACAAGAGAATGCTGTATTTCACAAGCCTCAGCCCTTCCAGTTCCAGCCTT GTGACAGTGATACTAGTTTAAAAGCCGCCCAACTGGTGGATATTGAGCTCTCCCCTGTCAGTGCTCTGAG AATGACGATAGCTGAGAGTCGACAATTAGAATCAGAAACTGGTACCACAGAGGAACACAGTCTAAATAAG GAGGCTCGGAAATGGGCCACACGTGTGGCACGTGAGCACAAAAATATTGTTCATCAGCAGCGGGTTCTAA ATGAACTAGAATGTCATGGAGTTGCTCTATCAGAACAGAGCCGAGCAGAGCTGGAACAGAAAATAGATGA AGCAAGAGAAAGTATCCGTAAAGCAGAGATCATTAAATTGAAAGCTGAAGCCCGGCTAGACCTACTGAAG CAGATTGGGGTTTCTGTAGATACGTGGTTAAAGAGTGCCATGAACCAAGTGATGGAGGAACTAGAAAATG AGCGGTGGGCCCGTCCTCCAGCCGTGACCAGCAATGGCACTCTACACTCGCTTAATGCAGATGCTGAAAG AGAAGAAGGAGAAGAGTTTGAAGACAACATGGATGTTTTTGATGACAGCAGCTCCAGCCCTTCTGGCACC TTGAGAAATTATCCACTCACCTGCAAAGTTGTTTATTCCTATAAGGCTTCTCAACCAGATGAGTTGACCA TTGAGGAACATGAGGTGTTAGAAGTGATTGAAGATGGAGATATGGAAGACTGGGTAAAGGCTCGAAATAA AGTTGGCCAAGTGGGTTATGTGCCAGAAAAGTACCTACAATTTCCCACCTCGAACAGCCTACTGAGCATG CTACAGTCTTTGGCTGCTTTGGACAGTCGGTCACACACATCCAGCAATTCCACAGAAGCAGAACTCGTTT CAGGCAGCCTGAATGGAGATGCCAGTGTCTGTTTTGTGAAAGCACTTTATGATTATGAGGGCCAGACAGA TGATGAGCTGTCCTTTCCCGAGGGAGCAATTATTCGCATCTTGAACAAAGAAAACCAAGATGATGATGGC TTCTGGGAAGGGGAGTTCAGTGGTCGAATCGGTGTTTTCCCATCGGTGCTAGTGGAAGAGCTCTCAGCCT CCGAGAATGGCGACACTCCATGGACAAGAGAGATCCAGATCTCTCCATCCCCCAAGCCTCACACATCCCT GCCTCCACTGCCTCTGTATGACCAGCCACCCAGCAGCCCGTATCCCAGTCCAGATAAGAGGAGCTCCCAG TTCTTCCCCCGGTCTCCTTCAGCCAATGAAAACAGCCTTCATGCTGAATCACCAGGATTCTCACAGGCCT CAAGACAGACTCCTGACACCTCATATGGCAAGCTGCGGCCTGTTCGGGCGGCGCCGCCACCACCCACACA GAACCACCGGCGGACAACTGAGAAGATGGAGGACGTGGAGATCACACTGGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_199012 |
| Insert Size | 2295 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_199012.2, NP_950177.2 |
| RefSeq Size | 4432 bp |
| RefSeq ORF | 2295 bp |
| Locus ID | 207278 |
| UniProt ID | Q3USJ8 |
| Gene Summary | Adapter protein that plays a role in endocytosis via clathrin-coated pits. Contributes to the internalization of cell surface receptors, such as integrin ITGB1 and transferrin receptor. Promotes endocytosis of EGFR in cancer cells, and thereby contributes to the down-regulation of EGFR signaling. Recruited to clathrin-coated pits during a mid-to-late stage of assembly, where it is required for normal progress from U-shaped intermediate stage pits to terminal, omega-shaped pits. Binds to membranes enriched in phosphatidylinositol 3,4-bisphosphate or phosphatidylinositol 3,4,5-trisphosphate (By similarity). When bound to membranes, promotes actin polymerization via its interaction with WAS and/or WASL which leads to the activation of the Arp2/3 complex (PubMed:23437151). Does not promote actin polymerisation in the absence of membranes (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG210309 | Fchsd2 (tGFP-tagged) - Mouse FCH and double SH3 domains 2 (Fchsd2) |
CNY 7320.00 |
|
| MR210309 | Fchsd2 (Myc-DDK-tagged) - Mouse FCH and double SH3 domains 2 (Fchsd2), transcript variant 1 |
CNY 5320.00 |
|
| MR210309L3 | Lenti ORF clone of Fchsd2 (Myc-DDK-tagged) - Mouse FCH and double SH3 domains 2 (Fchsd2), transcript variant 1 |
CNY 8550.00 |
|
| MR210309L4 | Lenti ORF clone of Fchsd2 (mGFP-tagged) - Mouse FCH and double SH3 domains 2 (Fchsd2), transcript variant 1 |
CNY 8550.00 |
