Poli (NM_011972) Mouse Untagged Clone
CAT＃: MC221231
Poli (untagged) - Mouse polymerase (DNA directed), iota (Poli), transcript variant 2, (10ug)
CNY 9600.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | Rad30b |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC221231 representing NM_011972
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGTGGAGTCGGAGGAGGAAGGCGGCCCCGCGGAGGAAGAAGACGCTCCTCGTGCCATGGAGCCCT TGCACGCGGGGGCGGCGGGCAGCTCGCGGGCAGTTTGCAGTCAAGGGCCACCTACACAAATCTCTTCGTC GAGAGTCATAGTCCACGTAGATCTGGATTGCTTTTATGCCCAGGTCGAAATGATCTCCAACCCAGAATTA AAGGACAGACCTTTAGGTGTGCAACAGAAATACTTGGTGGTTACCTGCAACTATGAAGCCAGGAAACTGG GTGTGAGGAAGCTTATGAATGTGAGAGATGCGAAGGAAAAGTGTCCTCAGCTGGTCCTCGTGAACGGAGA AGACCTGAGCCGCTACAGAGAGATGTCCTATAAGGTCACAGAATTGTTGGAGGAATTTAGTCCAGCTGTT GAGAGACTTGGATTTGATGAAAATTTTGTGGATCTAACAGAAATGGTGGAAAAAAGACTCCAGCAGCTTC CAAGTGAGGAGGTTCCGTCAGTGACTGTGTTCGGCCATGTTTACAATAACCAGTCTGTGAACCTACACAA CATCATGCATCGGAGACTTGTGGTTGGATCGCAGATTGCAGCAGAGATGCGGGAAGCCATGTATAATCAG CTCGGGCTCACAGGCTGCGCCGGAGTGGCTCCTAATAAACTCTTGGCAAAGTTAGTGTCTGGGGTTTTTA AACCAAATCAGCAGACGGTCTTACTACCTGAAAGTTGTCAACATCTCATTCACAGTTTGAACCACATAAA GGAAATTCCTGGTATCGGCTATAAAACTGCCAAGCGTCTCGAAGTTCTGGGAATCAATAGTGTTCATGAT CTCCAAACCTTTCCAATCAAAACATTAGAAAAAGAATTAGGAATTGCAATTGCTCAGCGTATCCAGCAGC TCAGTTTTGGAGAGGACAAGTCTCCCGTCACACCGTCAGGGCCACCACAGTCCTTTAGTGAAGAAGATAC GTTTAAAAAGTGTTCCTCAGAAGTGGAAGCTAAAGCTAAGATTGAAGAATTACTTTCCAGCCTTTTGACC AGAGTATGCCAGGATGGAAGGAAGCCCCATACAGTAAGATTAGTCATCCGTCGGTACTCTGACAAACACT GTAATCGAGAGAGTCGTCAGTGCCCTATCCCATCCCACGTCATTCAGAAGTTAGGGACAGGAAATCATGA CTCCATGCCTCCCCTGATTGATATCCTTATGAAACTTTTCCGAAATATGGTGAACGTGAAGATGCCCTTT CACCTGACTCTTATGAGCGTGTGCTTCTGCAACCTGAAAGCCCTGAGCAGTGCTAAGAAAGGGCCTATGG ACTGCTACCTAACGTCCCTCTCAACCCCTGCCTACACCGACAAGCGCGCCTTTAAAGTGAAGGACACCCA CACGGAAGACTCTCACAAGGAGAAAGAAGCAAACTGGGATTGTCTACCAAGTAGAAGAATCGAAAGCACA GGAACGGGGGAGTCTCCGTTGGATGCCACCTGTTTTCCTAAAGAAAAAGACACAAGTGACTTGCCCCTCC AGGCACTGCCAGAGGGTGTTGATCAAGAAGTCTTTAAGCAACTTCCAGCAGATATTCAAGAAGAAATCCT TTCTGGAAAATCTAGAGAAAATCTAAAAGGGAAAGGAAGTTTAAGTTGTCCGCTGCATGCCTCTAGAGGA GTATTGTCTTTCTTTTCTACAAAGCAAATGCAAGCCAGTCGCTTAAGCCCCAGAGATACTGCGCTCCCTA GCAAGCGGGTATCAGCTGCATCTCCCTGTGAGCCGGGAACGTCAGGACTGAGCCCCGGGAGCACCTCCCA TCCATCGTGCGGAAAGGACTGTTCCTATTACATAGACAGCCAGTTAAAGGATGAGCAAACGAGTCAAGGC CCTACTGAGTCTCAAGGATGCCAGTTTTCCAGCACGAACCCTGCTGTTTCTGGTTTCCATTCCTTTCCTA ATCTGCAGACTGAACAGCTCTTCTCCACACATCGCACTGTAGACAGCCACAAGCAGACAGCCACCGCCTC TCACCAGGGACTAGAAAGTCACCAGGGACTAGAAAGCAGAGAGCTGGATTCTGCGGAAGAAAAGCTTCCT TTCCCACCAGACATTGATCCGCAAGTTTTCTATGAGCTTCCAGAAGAGGTCCAAAAGGAACTGATGGCGG AATGGGAGAGAGCTGGAGCTGCGCGCCCCTCGGCGCACAGATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_011972 |
| Insert Size | 2214 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_011972.2, NP_036102.2 |
| RefSeq Size | 2482 bp |
| RefSeq ORF | 2214 bp |
| Locus ID | 26447 |
| Gene Summary | Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) encodes the longest isoform (2). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG216260 | Poli (tGFP-tagged) - Mouse polymerase (DNA directed) iota (Poli) transcript variant 2, (10ug) |
CNY 6370.00 |
|
| MR216260 | Poli (Myc-DDK-tagged) - Mouse polymerase (DNA directed), iota (Poli), transcript variant 2 |
CNY 5408.00 |
|
| MR216260L3 | Lenti ORF clone of Poli (Myc-DDK-tagged) - Mouse polymerase (DNA directed), iota (Poli), transcript variant 2 |
CNY 7700.00 |
|
| MR216260L4 | Lenti ORF clone of Poli (mGFP-tagged) - Mouse polymerase (DNA directed), iota (Poli), transcript variant 2 |
CNY 7700.00 |
