Gabbr1 (BC056990) Mouse Untagged Clone
CAT＃: MC220651
Gabbr1 (untagged) - Mouse gamma-aminobutyric acid (GABA-B) receptor, 1 (cDNA clone MGC:66925 IMAGE:6821442), (10ug)
CNY 9120.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | GABAbR1, bM573K1.1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC056990
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCCCGGGGGGACCCTGTACCCCGGTGGGGTGGCCGCTGCCTCTTCTGCTGGTGATGGCGGCTGGGG TGGCTCCGGTGTGGGCCTCTCACTCCCCTCATCTCCCGCGGCCTCACCCGAGGGTCCCCCCGCACCCCTC CTCAGAACGGCGTGCAGTATACATCGGGGCGCTGTTTCCCATGAGCGGGGGCTGGCCGGGGGGCCAGGCC TGCCAGCCTGCGGTGGAGATGGCGCTGGAGGACGTTAACAGCCGCAGAGACATCCTGCCGGACTACGAGC TCAAGCTTATCCACCACGACAGCAAGTGCGACCCAGGGCAAGCCACCAAGTACTTGTATGAACTACTCTA CAACGACCCCATCAAGATCATCCTCATGCCCGGCTGCAGCTCTGTGTCCACACTGGTAGCCGAGGCTGCC CGGATGTGGAACCTTATTGTGCTCTCATATGGCTCCAGCTCACCAGCCTTGTCAAACCGACAGCGGTTTC CAACGTTCTTTCGGACACATCCATCCGCCACACTCCACAATCCCACCCGGGTGAAACTCTTCGAAAAGTG GGGCTGGAAGAAGATTGCCACCATCCAGCAGACTACCGAGGTCTTCACCTCAACACTGGATGACCTGGAG GAGCGAGTGAAAGAGGCTGGGATTGAGATCACTTTTCGACAGAGTTTCTTCTCAGATCCAGCTGTGCCTG TTAAAAACCTGAAGCGTCAAGATGCTCGAATCATCGTGGGACTTTTCTATGAGACCGAAGCCCGGAAAGT TTTTTGTGAGGTCTATAAGGAACGGCTCTTTGGGAAGAAGTATGTCTGGTTTCTCATCGGGTGGTATGCT GACAACTGGTTCAAAACCTATGACCCGTCAATCAATTGTACAGTAGAAGAGATGACTGAGGCGGTGGAGG GCCATATCACCACGGAGATTGTCATGCTGAACCCTGCCAACACCCGAAGCATTTCCAACATGACATCACA GGAATTTGTGGAGAAACTAACCAAGCGGCTGAAAAGACACCCTGAGGAGACTGGAGGCTTCCAGGAGGCA CCACTGGCCTATGATGCTATCTGGGCCTTGGCTTTGGCCTTGAACAAGACCTCTGGAGGAGGTGGCCGTT CAGGAGTGCGCCTGGAGGACTTTAACTACAACAACCAGACCATTACAGACCAAATCTACCGGGCCATGAA CTCCTCCTCCTTTGAGGGTGTTTCTGGCCACGTGGTCTTTGATGCCAGCGGCTCCCGGATGGCATGGACG CTTATCGAGCAGCTACAGGGCGGCAGCTACAAGAAGATCGGCTACTACGACAGCACCAAGGATGATCTTT CCTGGTCCAAAACAGACAAGTGGATCGGAGGGTCTCCCCCAGCCGACCAGACCTTGGTCATCAAGACATT CCGTTTCCTGTCACAGAAACTCTTTATCTCCGTCTCAGTTCTCTCCAGCCTGGGCATTGTTCTTGCTGTT GTCTGTCTGTCCTTTAACATCTACAACTCCCACGTTCGTTATATCCAGAACTCCCAGCCCAACCTGAACA ATCTGACTGCTGTGGGCTGCTCACTGGCACTAGCTGCTGTCTTCCCCCTTGGGCTGGATGGTTACCACAT AGGGAGAAGCCAGTTCCCATTTGTCTGCCAGGCCCGCCTTTGGCTCTTGGGCTTAGGCTTTAGTCTGGGC TATGGCTCTATGTTCACCAAGATCTGGTGGGTCCACACAGTCTTCACAAAGAAGGAGGAGAAGAAGGAAC GGAGGAAGACCCTAGAGCCTTGGAAACTGTACGCCACTGTAGGCCTGCTGGTGGGCATGGATATCCTGAC TCTTGCCATCTGGCAGATTGTGGACCCCTTGCACCGAACCATTGAGACTTTTGCCAAGGAGGAACCAAAG GAAGACATTGATGTCTCCATTCTGCCCCAGCTGGAGCACTGCAGCTCCAAGAAGATGAATACATGGCTCG GCATTTTCTATGGTTACAAAGGGCTGCTGCTGCTGCTGGGAATCTTTCTTGCTTATGAGACCAAAAGTGT GTCCACTGAAAAGATCAATGACCACAGGGCCGTGGGCATGGCTATCTACAATGTTGCGGTCCTGTGTCTC ATTACCGCTCCTGTCACCATGATCCTTTCCAGCCAGCAGGACGCAGCCTTCGCCTTTGCCTCTCTGGCCA TTGTGTTCTCTTCCTACATTACTCTGGTTGTGCTCTTTGTGCCTAAGATGCGCAGGTTGATCACTCGAGG TGAATGGCAGTCTGAAGCACAGGACACAATGAAAACAGGATCATCCACCAACAACAATGAGGAAGAGAAG TCCCGACTGTTGGAGAAGGAAAACCGTGAATTGGAAAAGATCATCGCCGAGAAAGAGGAGCGTGTCTCTG AACTGCGCCATCAGCTCCAGTCTCGGCAGCAGATCCGCTCCCGGCGCCACCCCCCAACACCCCCAGACCC CTCTGGGGGCCTTCCCAGGGGACCCTCAGAGCCCCCTGACCGGCTTAGCTGTGATGGGAGTCGAGTACAT TTGCTTTACAAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | BC056990 |
| Insert Size | 2535 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC056990, AAH56990 |
| RefSeq Size | 3883 bp |
| RefSeq ORF | 2534 bp |
| Locus ID | 54393 |
| Gene Summary | Component of a heterodimeric G-protein coupled receptor for GABA, formed by GABBR1 and GABBR2 (PubMed:10773016, PubMed:10075644). Within the heterodimeric GABA receptor, only GABBR1 seems to bind agonists, while GABBR2 mediates coupling to G proteins (By similarity). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase (PubMed:10773016, PubMed:10075644). Signaling inhibits adenylate cyclase, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipid hydrolysis (PubMed:10075644). Calcium is required for high affinity binding to GABA (By similarity). Plays a critical role in the fine-tuning of inhibitory synaptic transmission (By similarity). Pre-synaptic GABA receptor inhibits neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA receptor decreases neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials (PubMed:10075644). Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG210927 | Gabbr1 (tGFP-tagged) - Mouse gamma-aminobutyric acid (GABA-B) receptor, 1 (cDNA clone MGC:66925 IMAGE:6821442) |
CNY 5320.00 |
|
| MR210927 | Gabbr1 (Myc-DDK-tagged) - Mouse gamma-aminobutyric acid (GABA-B) receptor, 1 (cDNA clone MGC:66925 IMAGE:6821442) |
CNY 6184.00 |
|
| MR210927L3 | Lenti ORF clone of Gabbr1 (Myc-DDK-tagged) - Mouse gamma-aminobutyric acid (GABA-B) receptor, 1 (cDNA clone MGC:66925 IMAGE:6821442) |
CNY 6750.00 |
|
| MR210927L4 | Lenti ORF clone of Gabbr1 (mGFP-tagged) - Mouse gamma-aminobutyric acid (GABA-B) receptor, 1 (cDNA clone MGC:66925 IMAGE:6821442) |
CNY 6750.00 |
