Sirt1 (NM_001159589) Mouse Untagged Clone
CAT＃: MC220605
Sirt1 (untagged) - Mouse sirtuin 1 (silent mating type information regulation 2, homolog) 1 (S. cerevisiae) (Sirt1), transcript variant 2, (10ug)
CNY 5120.00
CNY 9030.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AA673258; S; Si; Sir2; Sir2a; Sir2alpha; SIR2L1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>NM_001159589.1
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGACGAGGTGGCGCTCGCCCTTCAGGCCGCCGGCTCCCCTTCCGCGGCGGCCGCCATGGAGGCCG CGTCGCAGCCGGCGGACGAGCCGCTCCGCAAGAGGCCCCGCCGAGACGGGCCTGGCCTCGGGCGCAGCCC GGGCGAGCCGAGCGCAGCAGTGGCGCCGGCGGCCGCGGGGTGTGAGGCGGCGAGCGCCGCGGCCCCGGCG GCGCTGTGGCGGGAGGCGGCAGGGGCGGCGGCGAGCGCGGAGCGGGAGGCCCCGGCGACGGCCGTGGCCG GGGACGGAGACAATGGGTCCGGCCTGCGGCGGGAGCCGAGGGCGGCTGACGACTTCGACGACGACGAGGG CGAGGAGGAGGACGAGGCGGCGGCGGCAGCGGCGGCGGCAGCGATCGGCTACCGAGGTCCATATACTTTT GTTCAGCAACATCTCATGATTGGCACCGATCCTCGAACAATTCTTAAAGATTTATTACCAGAAACAATTC CTCCACCTGAGCTGGATGATATGACGCTGTGGCAGATTGTTATTAATATCCTTTCAGAACCACCAAAGCG GAAAAAAAGAAAAGATATCAATACAATTGAAGATGCTGTGAAGTTACTGCAGGAGTGTAAAAAGATAATA GTTCTGACTGGAGCTGGGGTTTCTGTCTCCTGTGGGATTCCTGACTTCAGATCAAGAGACGGTATCTATG CTCGCCTTGCGGTGGACTTCCCAGACCTCCCAGACCCTCAAGCCATGTTTGATATTGAGTATTTTAGAAA AGACCCAAGACCATTCTTCAAGTTTGCAAAGGAAATATATCCCGGACAGTTCCAGCCGTCTCTGTGTCAC AAATTCATAGCTTTGTCAGATAAGGAAGGAAAACTACTTCGAAATTATACTCAAAATATAGATACCTTGG AGCAGGTTGCAGGAATCCAAAGGATCCTTCAGTGTCATGGTTCCTTTGCAACAGCATCTTGCCTGATTTG TAAATACAAAGTTGATTGTGAAGCTGTTCGTGGAGACATTTTTAATCAGGTAGTTCCTCGGTGCCCTAGG TGCCCAGCTGATGAGCCACTTGCCATCATGAAGCCAGAGATTGTCTTCTTTGGTGAAAACTTACCAGAAC AGTTTCATAGAGCCATGAAGTATGACAAAGATGAAGTTGACCTCCTCATTGTTATTGGATCTTCTCTGAA AGTGAGACCAGTAGCACTAATTCCAAGTTCTATACCCCATGAAGTGCCTCAAATATTAATAAATAGGGAA CCTTTGCCTCATCTACATTTTGATGTAGAGCTCCTTGGAGACTGCGATGTTATAATTAATGAGTTGTGTC ATAGGCTAGGTGGTGAATATGCCAAACTTTGTTGTAACCCTGTAAAGCTTTCAGAAATTACTGAAAAACC TCCACGCCCACAAAAGGAATTGGTTCATTTATCAGAGTTGCCACCAACACCTCTTCATATTTCGGAAGAC TCAAGTTCACCTGAAAGAACTGTACCACAAGACTCTTCTGTGATTGCTACACTTGTAGACCAAGCAACAA ACAACAATGTTAATGATTTAGAAGTATCTGAATCAAGTTGTGTGGAAGAAAAACCACAAGAAGTACAGAC TAGTAGGAATGTTGAGAACATTAATGTGGAAAATCCAGATTTTAAGGCTGTTGGTTCCAGTACTGCAGAC AAAAATGAAAGAACTTCAGTTGCAGAAACAGTGAGAAAATGCTGGCCTAATAGACTTGCAAAGGAGCAGA TTAGTAAGCGGCTTGAGGGTAATCAATACCTGTTTGTACCACCAAATCGTTACATATTCCACGGTGCTGA GGTATACTCAGACTCTGAAGATGACGTCTTGTCCTCTAGTTCCTGTGGCAGTAACAGTGACAGTGGCACA TGCCAGAGTCCAAGTTTAGAAGAACCCTTGGAAGATGAAAGTGAAATTGAAGAATTCTACAATGGCTTGG AAGATGATACGGAGAGGCCCGAATGTGCTGGAGGATCTGGATTTGGAGCTGATGGAGGGGATCAAGAGGT TGTTAATGAAGCTATAGCTACAAGACAGGAATTGACAGATGTAAACTATCCATCAGACAAATCATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001159589 |
| Insert Size | 2097 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001159589.1, NP_001153061.1 |
| RefSeq Size | 3806 bp |
| RefSeq ORF | 2097 bp |
| Locus ID | 93759 |
| Gene Summary | This gene encodes a member of the sirtuin family of proteins, characterized by their deacetylase activity and proposed role in longevity. The encoded protein regulates gene expression in a wide range of cell and tissue types through its NAD+-dependent deacetylation of histones, transcription factors and transcriptional coactivators. Brain-specific overexpression of this gene has been shown to result in increased median lifespan. Viability of homozygous knockout mice for this gene varies with strain background. Homozygous knockout mice of strains that do not exhibit embryonic lethality are sterile and have a reduced lifespan. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015] Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG227266 | Sirt1 (tGFP-tagged) - Mouse sirtuin 1 (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) (Sirt1) transcript variant 2, (10ug) |
CNY 7224.00 |
|
| MR227266 | Sirt1 (Myc-DDK-tagged) - Mouse sirtuin 1 (silent mating type information regulation 2, homolog) 1 (S. cerevisiae) (Sirt1), transcript variant 2 |
CNY 6940.00 |
|
| MR227266L3 | Lenti ORF clone of Sirt1 (Myc-DDK-tagged) - Mouse sirtuin 1 (silent mating type information regulation 2, homolog) 1 (S. cerevisiae) (Sirt1), transcript variant 2 |
CNY 8840.00 |
|
| MR227266L4 | Lenti ORF clone of Sirt1 (mGFP-tagged) - Mouse sirtuin 1 (silent mating type information regulation 2, homolog) 1 (S. cerevisiae) (Sirt1), transcript variant 2 |
CNY 8840.00 |
