Nphp1 (NM_016902) Mouse Untagged Clone
CAT＃: MC220506
Nphp1 (untagged) - Mouse nephronophthisis 1 (juvenile) homolog (human) (Nphp1), (10ug)
CNY 7600.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC220506 representing NM_016902
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCGCCCGGCGGCCGCGGGACCCGCTGCAGGCGCTGAGGCGCCGCGGGCAGGAGCTGAAGCTGCAGG TTGATAGTTTGGTGACTGAAAGCCAACTAACAGGTGCTCTGGAACCGAGTAAAAGGAGAGAGATTTACCA AAGATGTATCCAGTTAAAACAGGCAGTAGATGAAAATAAAAACACCCTTCAAAAATTAAACAAAGCTGAT GAAGCTGCACCTGTGGGGAACTATGAGCAGAGGAAGGAGGAGGAACACAGCCTTCTGGAGAAGCTAGCCT GCCAGCTGCAGGAGCTTGCCGTGAGCATCAGCAGGAAAGACGCTCTCAAAGTTGAGGCACACAGTGACAA GGAGGAAGATGACACGACTGAAGATGATGAAGAAGAAACTGGTGGAGAGGAAGAAGAGTCAGAAGGGGAT GGAGAAGGAAAGCAAGAGCAAGCCTCCCCGAAACAAGCAGAAACAGAAACTGTCACATACATCGCTCTTG GGGACTTCGCTGCTCAGCAGACTGGGGACTTGACCTTTAAGAAAGGGGACGTTCTCCATATAATTGAAAA GAAGCCTGATGGTTGGTGGCTGGCTAAGGATGCTGAAGGAGTTGAGGGTCTCATTCCCAGGACCTACTTG GAGCCCTATAATAAAGAAGACAAGCTGGAGTCAAGCGAAGGCAGCGAGGAGGGCGGTGAGGAGGACGGTG AGGAGGACGGTGAGGAGGACGTGGAGGTGGTGGATGAAACTGCAGATGGAGCCCAGGTGAAGCAGAGAAC TGATTCCCACTGGAGTGCTGTACGAAAAGCTATCTCAGAGCAGATAAATACCGTTGATGTGTTAGCCACA ATGGGAGCCATTCCTGCAGGATTCCGGCCTTCCACGCTCTCCCAGCTGCTGGATGAAGCAGGGAACCAGT TTCGAGCAAGTTACTTCTTGCAGCCAGAGCTCACGACATCGCAGCTGGCCTTCAGAGATCTAACATGGGA TGCTAAGGCAGGCACGATCATGTCGAGACCAAGTCGTGTTTCATTGATTCTGACCCTATGGAGCTGTAAA ATGATCCCTCTCCCAGGAACAAGCATACAGGTTCTCAGCAGACACATCCGCCTCTGCCTGTTTGACGGCA GCAAGGTCCTGAGTAATATCCATACAGTTCGAGCCGTGTGGCAGCCTAAGAAGCCAAAAACATGGACCTT CTCTCCTCAGGTCACTGGCATCTTGCCATGCTTGCTCGATGGTGACTGTTTTATCAGGTCCAATTCTTCA ACTCCAGACCTTGGGATATTATTTGAACTTGGAATTTCTTACATTCGCAATTCAACTGGTGAGAGAGGAG AGTTAAGCTGTGGCTGGGTGTTTCTTAAGCTTTTCGATGCCAGTGGAGTTCCTATTCCAGCAAAAACATA CGAACTCTTCTTGAATGGCGGCACTCCTTACGAAAAAGGTGTTGAAGTGGACCCTTCAGTGTCCAGAAGA GCACAGGGCAGTGTTTTCCGTCAGATGATAAGTGTGCGCAGGCAGCCTCAACTTCTGGTGAAACTTCGGT CTCTGAACAGGAGGTCCAGGGCCATGCTCAGTCTGCTGCCAGAAACCCTGGTGGGGAGTATGTGCTCTGC TCACTTACTGATATTTTACCGACAAATTCTTGGAGATGTGCTCCTGAGAGACAGGACAAACCTGCAAAGT GCCGATTTAATCAGTCATCCCGTGCTGGCCACCTTCCCTCTGCTCCTGGAGCAGCCCGACGTGATGGATG CTCTCAGGAGTTCCTGGTCTGAGAAAGAAAGTACACTAAAGCGAGCAGAGAAGAGAGACAAAGAGCTCCT AAAGGCTGAGTTCCTCCTGGTCTACCATGACTGCGTGCTCCCTCTTCTGCACTCGACACTGCTGCCCCCC TTTAGGTGGGCCGAGGAAGAGACAGAGGCTGCAAGATGGAAAGCCATTGCTGACTTCCTCAAGCAGAGCC GGGAGAACGAGGGTTCCCTGAAGGCTCTGCTGTCACCAGATGGAGTTCACAAACCATTTGACCTTTCGGA GCAGACCTTTGACTTCTTGGGTGAAATAAGAAAGAACTCAGGCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_016902 |
| Insert Size | 2076 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_016902.3, NP_058598.1 |
| RefSeq Size | 2303 bp |
| RefSeq ORF | 2076 bp |
| Locus ID | 53885 |
| UniProt ID | Q9QY53 |
| Gene Summary | Together with BCAR1 it may play a role in the control of epithelial cell polarity. Involved in the organization of apical junctions in kidney cells, together with NPHP4 and RPGRIP1L/NPHP8 (PubMed:21565611). Does not seem to be strictly required for ciliogenesis (PubMed:19208653). Seems to help to recruit PTK2B/PYK2 to cell matrix adhesions, thereby initiating phosphorylation of PTK2B/PYK2 and PTK2B/PYK2-dependent signaling (PubMed:11493697). May play a role in the regulation of intraflagellar transport (IFT) during cilia assembly (PubMed:19208653). Required for normal retina development (PubMed:19208653). In connecting photoreceptor cilia influences the movement of some IFT proteins such as IFT88 and WDR19 (PubMed:19208653). Involved in spermatogenesis; required for the differentiation of early elongating spermatids into spermatozoa (PubMed:18684731).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG223034 | Nphp1 (tGFP-tagged) - Mouse nephronophthisis 1 (juvenile) homolog (human) (Nphp1), (10ug) |
CNY 7600.00 |
|
| MR223034 | Nphp1 (Myc-DDK-tagged) - Mouse nephronophthisis 1 (juvenile) homolog (human) (Nphp1) |
CNY 7592.00 |
|
| MR223034L3 | Lenti ORF clone of Nphp1 (Myc-DDK-tagged) - Mouse nephronophthisis 1 (juvenile) homolog (human) (Nphp1) |
CNY 8840.00 |
|
| MR223034L4 | Lenti ORF clone of Nphp1 (mGFP-tagged) - Mouse nephronophthisis 1 (juvenile) homolog (human) (Nphp1) |
CNY 8840.00 |
