Rnf145 (NM_028862) Mouse Untagged Clone
CAT＃: MC220270
Rnf145 (untagged) - Mouse ring finger protein 145 (Rnf145), transcript variant 1, (10ug)
CNY 8550.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 3732413I11Rik; TMRF1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC220270 representing NM_028862
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCGAAGGAGAAACTGGAGGCAGTGCTGAACGTGGCCTTGAGGGTGCCAAGCATCATGCTGCTGG ATGTCCTTTACCGCTGGGACGTCAGCTCCTTTTTCCAGCAGATCCAAAGAAGCAGCCTCAACAACAACCC TCTTTTCCAGTACAAATACTTGGCTCTTAATATGCATTACGTAGGTTATATCTTAAGTGTCGTGCTCCTC ACTCTGCCCAGGCAGCATCTGGTGCAGCTCTATTTGTATTTTGTGACTGCCCTGCTCCTCTATGCTGGAC ATCAGATATCCAGGGACTACGTAAGGAGTGAACTGGAGTCTGCCTATGAAGGACCAATGTATTTAGAACC TCTCTCCATGAACCGCTTTACCACAGCCTTAATAGGTCAGTTAGTGGTGTGCACCTTGTGCTCCTGCGTT ATGAAGACGAAGCAGATCTGGCTCTTCTCGGCACACATGCTTCCTCTGCTGGCTCGCCTCTGCCTCGTTC CTCTGGAGACCATCGTCATCATCAACAAATTTGCTATGATTTTTACAGGATTGGAAGTCCTGTACTTCCT AGGGTCTAATCTCCTAGTACCGTATAACCTCGCTAAGTCTGCATACCGGGAGTTAGTTCAGGTAGTGGAG GTGTACGGCCTTCTGGCCTTGGGAATGTCCCTGTGGAACCAGCTGGTGGTGCCCGTGCTCTTCATGGTCT TCTGGCTTGTTTTATTTGCTCTTCAGATCTACTCCTACTTCAGCACCAGAGATCAGCCTGCATCCCGGGA GAGGCTTCTCTTCCTTTTCCTGACAAGCATCGCCGAGTGCTGCAGCACGCCCTACTCCCTTCTGGGCTTG GTCTTCACCGTGTCCTTTGTTGCCTTGGGTGTTCTGACACTCTGTAAGTTCTACCTGCAGGGATACCGAG CCTTCATGAACGATCCTGCCATGAATAGGGGGATGACAGAAGGAGTCACTCTGCTGATCCTGGCGGTGCA GACGGGGCTCATAGAACTTCAGGTGGTTCACCGTGCATTCCTGCTCAGCATCATCCTCTTCATCGTCGTT GCCTCTATCCTGCAGTCCATGTTGGAGATCGCAGATCCCATTGTTTTGGCACTGGGAGCATCGAGAGACA AGAGTTTGTGGAAACACTTCCGTGCTGTGAGCCTCTGCTTGTTCCTCCTCGTGTTCCCTGCGTATATGGC CTACATGATTTGCCAGTTCTTCCACATGGACTTCTGGCTTCTCATCATCATCTCCAGCAGCATCCTTACC TCTCTCCAGGTTCTGGGAACACTTTTTATTTACGTCTTATTTATGGTTGAGGAATTCCGCAAAGAGCCCG TGGAAAACATGGATGACGTCATCTACTACGTGAATGGCACTTATCGTCTGCTGGAGTTTGTTGTGGCCCT CTGTGTGGTGGCCTACGGCGTCTCAGAGACCATCTTTGGAGAGTGGACTGTGATGGGCTCCATGATCATC TTCATCCATTCCTACTACAACGTGTGGCTTCGTGCCCAGCTGGGCTGGAAGAGTTTCCTTCTGCGCAGGG ACGCTGTGAATAAGATTAAGTCTCTCCCTGTCGCGACGCAGGAGCAGCTGGAGAAACACAACGACATCTG CGCCATCTGTTACCAGGACATGAAATCCGCTGTGATCACACCTTGCAGTCATTTCTTCCATGCAGGATGT CTTAAGAAATGGCTGTATGTCCAGGATACCTGCCCTCTGTGTCACTGCCATCTCAAAAACTCCTCCCAGC TTCCAGGACTGGGGACAGAAGCGGCTCCACAGCCTCCTGCGGGGGCCGAGCAGAACATTGTACTTCAGGA AGGTCCTGAACCCCCGGACCATGAGAGTCCACCAGGGACTGGGACCCAGGAAGGTTCTGGGGACAGTAGT GAACACATCAACAGAGGCTCGGCTAGCCAGGAGGGGGCTGCTGATGCTGGAGAGGGTCCTCAGATTCCAG AAGGCGAAGTGTGTCCTGTTGAGTCTGCCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_028862 |
| Insert Size | 1992 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_028862.3, NP_083138.2 |
| RefSeq Size | 3584 bp |
| RefSeq ORF | 1992 bp |
| Locus ID | 74315 |
| UniProt ID | Q5SWK7 |
| Gene Summary | E3 ubiquitin ligase that catalyzes the direct transfer of ubiquitin from E2 ubiquitin-conjugating enzyme to a specific substrate. In response to bacterial infection, negatively regulates the phagocyte oxidative burst by controlling the turnover of the NADPH oxidase complex subunits. Promotes monoubiquitination of CYBA and 'Lys-48'-linked polyubiquitination and degradation of CYBB NADPH oxidase catalytic subunits, both essential for the generation of antimicrobial reactive oxygen species (PubMed:26194095). Involved in the maintenance of cholesterol homeostasis. In response to high sterol concentrations ubiquitinates HMGCR, a rate-limiting enzyme in cholesterol biosynthesis, and targets it for degradation. The interaction with INSIG1 is required for this function (PubMed:29374057). In addition, triggers ubiquitination of SCAP, likely inhibiting its transport to the Golgi apparatus and the subsequent processing/maturation of SREBPF2, ultimately downregulating cholesterol biosynthesis (PubMed:29068315).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longer isoform (1). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG221113 | Rnf145 (tGFP-tagged) - Mouse ring finger protein 145 (Rnf145) transcript variant 1, (10ug) |
CNY 5040.00 |
|
| MR221113 | Rnf145 (Myc-DDK-tagged) - Mouse ring finger protein 145 (Rnf145), transcript variant 1 |
CNY 4936.00 |
|
| MR221113L3 | Lenti ORF clone of Rnf145 (Myc-DDK-tagged) - Mouse ring finger protein 145 (Rnf145), transcript variant 1 |
CNY 6460.00 |
|
| MR221113L4 | Lenti ORF clone of Rnf145 (mGFP-tagged) - Mouse ring finger protein 145 (Rnf145), transcript variant 1 |
CNY 6460.00 |
