Zfp12 (NM_177681) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	C530015C18; Krox-7; Zfp-12; Znf12
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC220206 representing NM_177681 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATACATCCTTAGGCCCACTGTCATTCAAGGATGTGGCTGTGGCCTTCTCCCAGGAGGAGTGGCAGC AGCTGGACCCTGAGGAAAGGACGACATACAGGGATGTGATGCTGGAGACCTACAGCAACCTCGTCTCCGT GGATGAGATCCGGCACATGAGCAGGCTGATGGAGGAGGACCAGGGAGGTGAAGAGAACCAGTCAAGTTCA GCTGTGTTCAGCTACAGAAGCCGGGCTGATGCCTCTAGTAAGGCCACTGATGGAGAAACAAAGCCCTTTC CCTCACAGAAGGCCCTCCCTCAGTGCAACTCCTGTGAGAAGAGCCTGATGTGTGTCTCGGCGTTCATCCG AAGCGACGGAAGCTACGCAAAACTGAGGCCCAATGTGTGTGCCGGGTGTGGGAAGCCCCTCCCCTGCAGC AAGCCCGAGGAAACACACCCTGGAGGTGAGTCTTACGAATTTAGTGGGGATGGAGATGAGGACCCTCTCG GTGAAGAAGGCGTGTATCAGAAGGGTCACTTTTTGGAGGAGCCCTTTGAATACGTTGAGTGCCAGAAATC CTTCCCCAAGGGCACTGTGTTTCTTAATCACCTGGAAGAGGAGCCCTGCGACTGGAATGACGCTGAGGTC GCTTTCCTGCAGACGTCAGACCTCAGTGCCCACCAGGATTCTCTCATGGAAATGAAGCCCTACGAGTGCC AGCAGTGTGGGAAGTCCTTCTGCAAAAAGTCCAAGTTCGTCATCCATCAGAGGACTCACACAGGAGAGAA GCCCTTCAAGTGCAGTCAGTGTGGGAAGTCCTTCTGCCAGAAGGGCACCCTCACCGTCCACCAGAGGACG CACACCGGGGAGAAGCCCTACGAGTGTACCGAGTGCGGGAAGACCTTCTACCAGAAGCTGCACCTCATTC AGCACCAGAGGACCCACTCGGGCGAGAAGCCCTACAAGTGTGGCTACTGCGGGAAGTCCTTCTGCCAGAA GACGCATCTCACTCAGCACCAGAGGACGCACTCGGGAGAGAGGCCCTATGTTTGTCACGACTGTGGCAAG ACGTTCTCTCAGAAGTCAGCACTCAATGACCACCAGAAGATCCACACGGGCGTCAAACTCTACAAATGCA GCGAGTGTGGCAAGTGCTTCTGCCGCAAGTCCACGCTCACAACCCACATGAGGACGCACACGGGCGAGAA GCCGTACGAATGCAACGAGTGCGGCAAGTTCTTCTCCAGGCTGTCGTACCTCACCGTGCATTACAGAACC CACTCGGGGGAGAAGCCCTACGAGTGTGCGGAGTGCGGGAAGTCCTTCTACCTGAACTCAGCCCTTATGA GACATCAGAGGGTGCACACGGGGGAGAAGCCTTACGAGTGTAACGAGTGTGGGAAATTATTCTCGCAGCT GTCCTACCTCACCGTGCATCACAGAACTCACTCAGGAGTGAAGCCCTATGAGTGTAGCGAGTGTGGAAAG ACCTTCTACCAGAATTCAGCCCTCTGTAGACACCGGAGAATTCACAGAGGGGAGAAGCCCTATGAATGCT ACATCTGTGGCAAGTTCTTTTCTCAGATGTCTTACCTCACGATCCATCACAGAATTCACTCAGGGGAGAA GCCCTATGAGTGCCGCGAGTGTGGGAAGTCCTTCTGCCAGAACTCAGCCCTCAACAGACACCAGCGCACG CACACGGGAGAAAAAGCCTACGAGTGTTACGAGTGTGGGAAATGCTTCTCCCAGATGTCTTATCTCACTA TCCATCACAGGATCCACTCGGGAGAGAAGCCCTTCGAGTGTAACGAGTGTGGGAAAGCCTTCTCTCGGAT GTCGTACCTCACTGTGCACCACAGGACCCACTCAGGAGAGAAGCCCTATGAATGTACGGAGTGCGGGAAG AAATTCTACCACAAGTCCGCGTTCAATAGCCATCAGAGAACTCACAGGAGAGGGAGCGGGAATGGGGTTG ATTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_177681
Insert Size	1965 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_177681.4, NP_808349.1
RefSeq Size	5180 bp
RefSeq ORF	1965 bp
Locus ID	231866
UniProt ID	Q7TSI0
Gene Summary	Transcriptional repressor which suppresses activation protein 1 (AP-1)- and serum response element (SRE)-mediated transcriptional activity.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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