Itsn1 (NM_001110276) Mouse Untagged Clone
CAT＃: MC219800
Itsn1 (untagged) - Mouse intersectin 1 (SH3 domain protein 1A) (Itsn1), transcript variant 3, (10ug)
CNY 7980.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AA517634; AA545208; AI316805; AI839402; AI848451; Ehsh1; Ese1; Itsn; Sh3p17 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC219800 representing NM_001110276
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCAGTTTCCCACACCTTTCGGTGGTAGCCTGGATGTCTGGGCCATAACTGTGGAGGAAAGGGCCA AGCATGACCAGCAGTTCCTTAGCCTGAAGCCGATAGCGGGATTTATTACTGGTGATCAAGCGAGGAACTT TTTTTTCCAATCTGGGTTACCTCAGCCTGTCTTAGCACAAATATGGGCGCTAGCGGACATGAATAACGAT GGAAGGATGGATCAAGTGGAATTTTCCATAGCCATGAAGCTTATCAAACTGAAGCTACAAGGATATCAGC TCCCCTCCACACTTCCCCCTGTCATGAAACAGCAACCAGTGGCTATTTCCAGTGCACCAGCATTTGGTAT AGGAGGGATTGCTAGCATGCCACCACTCACAGCTGTTGCTCCTGTGCCAATGGGCTCCATTCCAGTTGTT GGAATGTCTCCACCCTTAGTATCTTCTGTCCCTCCAGCAGCAGTGCCTCCCCTGGCTAACGGGGCTCCTC CCGTCATACAGCCTCTGCCTGCGTTTGCGCATCCTGCAGCCACATTGCCAAAGAGTTCTTCCTTCAGCAG ATCTGGTCCAGGGTCACAATTAAACACTAAGTTACAGAAGGCACAATCATTCGATGTCGCCAGCGCCCCT CCAGCAGCAGAATGGGCTGTGCCTCAGTCATCAAGGCTGAAATACAGGCAGTTATTCAACAGCCACGACA AAACTATGAGTGGACACTTAACAGGTCCCCAGGCAAGAACTATTCTCATGCAATCAAGTTTACCCCAGGC TCAGCTGGCTTCAATATGGAATCTTTCTGACATTGATCAAGATGGAAAACTCACTGCAGAAGAATTTATC CTAGCTATGCACCTAATTGATGTTGCCATGTCTGGTCAGCCACTGCCGCCCGTCCTGCCTCCAGAATACA TCCCTCCTTCCTTCAGAAGAGTTCGCTCCGGCAGTGGGATGTCCGTCATAAGCTCTTCTTCTGTGGATCA GAGGCTGCCTGAGGAGCCGTCGTCAGAGGATGAGCAGCAGCCAGAGAAGAAACTGCCTGTGACATTTGAA GATAAGAAGCGGGAGAACTTCGAGCGAGGCAGTGTGGAGCTGGAGAAGCGCCGCCAAGCGCTCTTGGAGC AGCAGCGCAAAGAGCAGGAGCGGTTGGCTCAGCTGGAGCGCGCCGAGCAGGAGAGGAAAGAGCGGGAGCG CCAGGAGCAGGAGCGCAAGCGGCAGCTGGAGCTGGAGAAGCAGCTGGAGAAGCAGCGGGAGCTGGAGCGG CAGCGAGAGGAGGAGAGGAGGAAGGAGATCGAGAGGCGCGAGGCCGCAAAACGGGAACTGGAAAGGCAGC GACAACTTGAATGGGAACGGAACCGGAGACAGGAACTCCTGAATCAGAGGAACAAGGAGCAGGAGGGCAC CGTGGTCCTGAAGGCAAGGAGGAAGACTCTGGAGTTTGAGTTAGAAGCTCTGAATGACAAAAAGCATCAG CTAGAAGGAAAACTTCAGGATATCAGGTGTCGACTGGCAACCCAGAGGCAAGAAATTGAGAGCACGAACA AGTCTAGAGAGCTAAGAATTGCTGAAATCACCCACTTACAGCAGCAGTTGCAGGAATCTCAGCAAATGCT TGGAAGACTTATTCCAGAGAAACAGATACTCAGTGACCAGTTAAAACAAGTCCAGCAGAACAGTTTGCAT AGAGACTCGCTTCTTACCCTCAAAAGAGCCTTGGAAGCAAAGGAGCTGGCCCGGCAGCAGCTCCGGGAGC AGCTGGACGAGGTGGAGAGAGAGACCAGGTCAAAGCTGCAGGAGATTGATGTTTTCAACAACCAGCTGAA GGTGACTCCTGGGTGCTGGGCCGCCTCCCCTTTGCTTGGCTTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001110276 |
| Insert Size | 1866 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001110276.1, NP_001103746.1 |
| RefSeq Size | 6154 bp |
| RefSeq ORF | 1866 bp |
| Locus ID | 16443 |
| Gene Summary | Adapter protein that provides a link between the endocytic membrane traffic and the actin assembly machinery (PubMed:10064583). Acts as guanine nucleotide exchange factor (GEF) for CDC42, and thereby stimulates actin nucleation mediated by WASL and the ARP2/3 complex (By similarity). Plays a role in the assembly and maturation of clathrin-coated vesicles (By similarity). Recruits FCHSD2 to clathrin-coated pits (By similarity). Involved in endocytosis of activated EGFR, and probably also other growth factor receptors (PubMed:16914641). Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR may involve association with DAB2 (By similarity). Promotes ubiquitination and subsequent degradation of EGFR, and thereby contributes to the down-regulation of EGFR-dependent signaling pathways (PubMed:16914641). In chromaffin cells, required for normal exocytosis of catecholamines (PubMed:18676989). Required for rapid replenishment of release-ready synaptic vesicles at presynaptic active zones (PubMed:23633571). Inhibits ARHGAP31 activity toward RAC1 (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) has multiple differences in the presence and absence of exons at its 3' end, compared to variant 1. These differences produce a unique 3' UTR and result in a shorter protein (isoform 3) with a distinct C-terminus, compared to isoform 1. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG222569 | Itsn1 (tGFP-tagged) - Mouse intersectin 1 (SH3 domain protein 1A) (Itsn1) transcript variant 3, (10ug) |
CNY 5040.00 |
|
| MR222569 | Itsn1 (Myc-DDK-tagged) - Mouse intersectin 1 (SH3 domain protein 1A) (Itsn1), transcript variant 3 |
CNY 4624.00 |
|
| MR222569L3 | Lenti ORF clone of Itsn1 (Myc-DDK-tagged) - Mouse intersectin 1 (SH3 domain protein 1A) (Itsn1), transcript variant 3 |
CNY 6460.00 |
|
| MR222569L4 | Lenti ORF clone of Itsn1 (mGFP-tagged) - Mouse intersectin 1 (SH3 domain protein 1A) (Itsn1), transcript variant 3 |
CNY 6460.00 |
