Pias3 (NM_146135) Mouse Untagged Clone
CAT＃: MC219686
Pias3 (untagged) - Mouse protein inhibitor of activated STAT 3 (Pias3), transcript variant 1, (10ug)
CNY 7890.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | Pias3l |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC219686 representing NM_146135
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGATGAGTTTCCGAGTGTCTGAGCTCCAGGTGCTCCTCGGCTTCGCTGGCAGGAACAAGAGTGGGC GGAAACACGAGCTGCTGGCCAAGGCCCTGCACCTCCTCAAGTCTAGCTGCGCCCCCAGCGTCCAGATGAA GATCAAAGAACTTTATCGCAGGCGCTTTCCCCGGAAGACCCTGGGGCCTTCTGATCTCTCCTTGCTTTCT TTGCCCCCTGGCACCTCTCCTGTAGGCTCCCCCGGCCCCCTTGCTCCCATCCCTCCCACCCTTCTGACCC CTGGCACCTTGCTAGGCCCTAAGCGTGAGGTGGACATGCATCCTCCTCTGCCGCAGCCTGTGCACCCCGA TGTCACCATGAAGCCACTGCCCTTCTATGAAGTCTATGGGGAGCTCATCCGACCCACCACCCTTGCGTCC ACCTCCAGCCAGAGGTTCGAGGAAGCCCACTTCACCTTCGCGCTCACTCCCCAGCAGCTGCAGCAGATTC TCACGTCCAGGGAGGTTCTGCCAGGAGCCAAGTGTGATTACACCATACAAGTGCAGCTCAGATTCTGTCT CTGTGAGACCAGCTGCCCTCAGGAGGACTATTTCCCCCCTAACCTCTTTGTTAAGGTTAATGGGAAACTC TGCCCCCTGCCGGGTTACCTCCCTCCAACCAAGAATGGAGCTGAGCCCAAGAGGCCCAGCCGTCCGATCA ACATCACACCCTTGGCTCGACTCTCAGCCACTGTCCCCAACACCATCGTAGTTAATTGGTCATCTGAGTT TGGACGGAATTACTCCTTGTCCGTGTACCTGGTGAGGCAATTGACTGCAGGGACCCTTCTACAAAAACTC AGAGCCAAGGGGATCCGGAATCCAGACCATTCCCGGGCACTGATCAAGGAGAAACTGACTGCTGACCCCG ACAGTGAAGTGGCTACTACAAGTCTCCGGGTGTCACTCATGTGCCCGCTAGGGAAGATGCGCCTGACTGT CCCGTGTCGTGCCCTCACCTGTGCCCATCTGCAGAGTTTCGATGCTGCCCTTTATCTACAGATGAATGAG AAGAAGCCGACATGGACGTGTCCTGTGTGTGACAAGAAGGCTCCCTATGAGTCGCTGATTATTGATGGTT TATTCATGGAAATTCTTAATTCCTGTTCGGATTGTGATGAGATCCAGTTCATGGAAGATGGATCCTGGTG TCCGATGAAACCCAAGAAGGAGGCATCAGAGGTTTGCCCCCCGCCAGGGTATGGGCTGGATGGTCTCCAG TACAGCGCAGTCCAGGAGGGAATTCAGCCAGAGAGTAAGAAGAGGGTCGAAGTCATTGACTTGACCATCG AAAGCTCATCAGATGAGGAGGATTTGCCCCCCACCAAGAAGCACTGCCCTGTCACCTCAGCGGCCATTCC AGCCCTTCCTGGAAGCAAAGGAGCCCTGACCTCTGGTCACCAGCCATCCTCGGTGCTGCGGAGCCCTGCA ATGGGCACACTGGGCAGTGACTTCCTGTCTAGTCTCCCGCTACATGAGTACCCACCTGCCTTCCCACTGG GGGCTGACATCCAAGGTTTAGATTTATTTTCTTTCCTTCAGACTGAGAGTCAGCACTACGGCCCTTCAGT TATCACTTCGCTAGATGAACAGGACACCCTTGGCCACTTCTTCCAGTACCGGGGAACCCCTTCCCACTTC CTGGGCCCACTGGCCCCCACACTGGGGAGCTCTCACCGCAGCTCCACTCCAGCGCCCCCTCCTGGTCGTG TCAGCAGCATTGTGGCTCCTGGGAGCTCCTTGAGGGAAGGGCATGGAGGACCCCTGCCTTCAGGTCCCTC TTTGACTGGCTGTCGGTCAGACGTCATTTCCTTGGACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_146135 |
| Insert Size | 1860 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_146135.2, NP_666247.1 |
| RefSeq Size | 2975 bp |
| RefSeq ORF | 1860 bp |
| Locus ID | 229615 |
| UniProt ID | O54714 |
| Gene Summary | Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Repressor of STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. Repressor of MITF transcriptional activity. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. Sumoylates CCAR2 which promotes its interaction with SIRT1 (By similarity). Diminishes the sumoylation of ZFHX3 by preventing the colocalization of ZFHX3 with SUMO1 in the nucleus (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes isoform 1. |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG209486 | Pias3 (tGFP-tagged) - Mouse protein inhibitor of activated STAT 3 (Pias3), transcript variant 1 |
CNY 5040.00 |
|
| MR209486 | Pias3 (Myc-DDK-tagged) - Mouse protein inhibitor of activated STAT 3 (Pias3), transcript variant 1 |
CNY 4608.00 |
|
| MR209486L3 | Lenti ORF clone of Pias3 (Myc-DDK-tagged) - Mouse protein inhibitor of activated STAT 3 (Pias3), transcript variant 1 |
CNY 6460.00 |
|
| MR209486L4 | Lenti ORF clone of Pias3 (mGFP-tagged) - Mouse protein inhibitor of activated STAT 3 (Pias3), transcript variant 1 |
CNY 6460.00 |
