A1cf (NM_001081074) Mouse Untagged Clone
CAT＃: MC219534
A1cf (untagged) - Mouse APOBEC1 complementation factor (A1cf), (10ug)
CNY 7700.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 1810073H04Rik; Acf; ACF64; ACF65; ASP; MCM; mer; MerCreMer; Tg(Myh6-cre/Esr1*)1Jmk |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC219534 representing NM_001081074
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAATCAAATCACAAATCCGGGGATGGATTGAGCGGCACCCAGAAGGAAGCGGCACTCCGCGCACTGG TCCAGCGCACAGGATATAGCTTGGTCCAGGAAAATGGACAAAGGAAGTATGGTGGCCCTCCACCAGGCTG GGATTCTACACCCCCAGAAAGGGGCTGCGAGATTTTCATTGGGAAACTTCCCCGGGACCTCTTTGAGGAT GAACTCATACCATTGTGTGAAAAAATTGGTAAAATTTATGAAATGAGATTGATGATGGATTTTAATGGTA ACAACAGAGGCTATGCATTTGTAACATTCTCAAATAAGCAGGAAGCCAAGAATGCAATCAAGCAACTTAA TAATTATGAAATTCGGACTGGCCGTCTCTTGGGAGTCTGTGCCAGTGTGGACAATTGCCGATTGTTTGTG GGAGGAATCCCCAAAACCAAAAAGAGGGAAGAAATCTTATCAGAGATGAAAAAAGTCACAGAAGGAGTTG TTGATGTCATTGTCTACCCAAGTGCTGCTGATAAAACCAAAAACCGGGGATTTGCCTTTGTGGAATATGA GAGTCACCGAGCAGCCGCCATGGCTAGGCGGAGGCTGCTGCCAGGAAGAATTCAGTTGTGGGGACATCCT ATTGCAGTAGACTGGGCAGAACCAGAAGTTGAAGTTGATGAGGACACAATGTCTTCCGTGAAAATCCTGT ACGTAAGGAACCTTATGCTGTCTACCTCGGAAGAAATGATTGAAAAGGAATTCAACAGTATTAAACCAGG TGCTGTGGAAAGAGTGAAGAAGATCCGAGACTACGCTTTTGTGCACTTCAGTAACCGAGAAGATGCAGTT GAAGCCATGAAGGCTTTGAATGGCAAGGTGCTGGATGGTTCCCCAATAGAAGTGACCTTGGCCAAGCCAG TGGACAAGGACAGTTATGTTAGGTACACCCGGGGCACCGGTGGCAGGAACACCATGCTGCAAGGAGAATA CACCTACCCTCTGAGCCATGTTTATGACCCTACCACAACCTACCTTGGAGCTCCTGTCTTCTATACCCCC CAAGCCTATGCAGCCATTCCAAGTCTTCATTTCCCAGCTACTAAAGGACATCTCAGCAACAGGGCTCTCA TCAGGACCCCTTCTGTCAGAGAAATTTACATGAATGTCCCTGTAGGGGCTGCGGGGGTGAGAGGACTGGG CGGCCGTGGGTATTTGGCATACACAGGCCTGGGTCGAGGATACCACGTCAAAGGAGACAAGAGAGAAGAC AAACTCTATGATCTTCTGCCTGGGATGGAGCTCACCCCGATGAATACTGTCTCTTTAAAACCACAAGGAA TTAAACTTGCTCCTCAGATATTAGAAGAAATCTGTCAGAAAAATAACTGGGGACAGCCAGTGTACCAACT GCATTCTGCCATTGGACAAGATCAAAGACAGTTATTCCTCTACAAAGTAACTATCCCAGCGCTGGCCAGC CAGAATCCTGCAATCCACCCTTTCATACCACCAAAGCTAAGCGCCTATGTGGATGAAGCGAAGAGGTATG CTGCAGAGCACACTCTACAGACACTAGGCATCCCCACAGAAGGAGGGGACGCTGGGACTACAGCACCCAC GGCCACTTCTGCCACTGTGTTCCCAGGATATGCTGTCCCCAGTGCCACCGCTCCTGTGTCTACAGCCCAG CTCAAGCAAGCAGTGACACTTGGACAAGACTTAGCAGCATATACAACCTATGAGGTCTACCCTACTTTTG CACTGACCACCCGAGGCGATGCATATGGAACCTTCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001081074 |
| Insert Size | 1788 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001081074.1, NP_001074543.1 |
| RefSeq Size | 3828 bp |
| RefSeq ORF | 1788 bp |
| Locus ID | 69865 |
| UniProt ID | Q5YD48 |
| Gene Summary | Essential component of the apolipoprotein B mRNA editing enzyme complex which is responsible for the postranscriptional editing of a CAA codon for Gln to a UAA codon for stop in APOB mRNA. Binds to APOB mRNA and is probably responsible for docking the catalytic subunit, APOBEC1, to the mRNA to allow it to deaminate its target cytosine. The complex also seems to protect the edited APOB mRNA from nonsense-mediated decay (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG224176 | A1cf (tGFP-tagged) - Mouse APOBEC1 complementation factor (A1cf), (10ug) |
CNY 5040.00 |
|
| MR224176 | A1cf (Myc-DDK-tagged) - Mouse APOBEC1 complementation factor (A1cf) |
CNY 6648.00 |
|
| MR224176L1 | Lenti ORF clone of A1cf (Myc-DDK-tagged) - Mouse APOBEC1 complementation factor (A1cf) |
CNY 9048.00 |
|
| MR224176L2 | Lenti ORF clone of A1cf (mGFP-tagged) - Mouse APOBEC1 complementation factor (A1cf) |
CNY 6460.00 |
|
| MR224176L3 | Lenti ORF clone of A1cf (Myc-DDK-tagged) - Mouse APOBEC1 complementation factor (A1cf) |
CNY 6460.00 |
|
| MR224176L4 | Lenti ORF clone of A1cf (mGFP-tagged) - Mouse APOBEC1 complementation factor (A1cf) |
CNY 6460.00 |
