Slc1a2 (NM_001077515) Mouse Untagged Clone
CAT＃: MC219058
Slc1a2 (untagged) - Mouse solute carrier family 1 (glial high affinity glutamate transporter), member 2 (Slc1a2), transcript variant 2, (10ug)
CNY 7220.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 1700091C19Rik; 2900019G14Rik; AI159670; Eaat2; GLT-1; GLT1; MGLT1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC219058 representing NM_001077515
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCAGTGCCAACAATATGCCCAAGCAGGTAGAAGTGCGCATGCATGACAGCCACCTCAGCTCCGATG AGCCAAAGCACCGAAACCTGGGCATGCGCATGTGCGACAAGCTGGGGAAAAATCTCCTGCTCTCACTGAC TGTGTTTGGTGTCATCCTGGGAGCAGTGTGTGGCGGGCTGCTTCGCTTGGCATCGCCCATCCACCCTGAT GTGGTCATGTTGATAGCCTTCCCGGGGGACATACTCATGAGGATGCTGAAGATGCTCATCCTCCCTCTTA TCATCTCCAGTTTAATCACAGGGTTGTCAGGCCTGGATGCTAAAGCCAGCGGCCGCCTAGGCACGAGAGC TATGGTGTATTACATGTCCACGACCATCATTGCCGCCGTGCTGGGGGTCATCCTGGTGTTGGCCATCCAC CCAGGCAATCCCAAACTCAAGAAGCAGCTAGGGCCCGGGAAGAAGAACGACGAGGTGTCTAGCCTGGATG CCTTCCTGGATCTCATTAGAAATCTCTTCCCGGAGAACCTGGTGCAAGCCTGTTTCCAGCAGATTCAGAC AGTGACAAAGAAAGTTCTGGTGGCACCTCCATCTGAGGAGGCCAATACCACCAAGGCGGTCATCTCCATG TTGAATGAAACCATGAACGAGGCCCCTGAAGAAACTAAGATCGTTATCAAGAAGGGCCTGGAGTTCAAGG ACGGGATGAACGTCTTAGGTCTGATCGGATTCTTTATTGCTTTCGGCATTGCCATGGGGAAGATGGGTGA ACAGGCCAAGCTGATGGTGGAGTTCTTCAACATTCTGAATGAGATCGTGATGAAGTTAGTGATCATGATC ATGTGGTACTCCCCTCTGGGTATCGCCTGCTTGATTTGTGGGAAGATCATCGCCATCAAGGACTTAGAAG TGGTTGCTAGGCAGCTGGGGATGTACATGATCACCGTGATCGTGGGCCTCATCATTCACGGGGGCATCTT TCTCCCCTTGATTTACTTTGTAGTGACCAGAAAAAATCCATTCTCCTTTTTTGCTGGCATATTCCAAGCC TGGATCACTGCTCTGGGAACTGCTTCCAGTGCTGGAACTTTGCCTGTTACCTTCCGTTGCTTGGAAGATA ATCTAGGGATTGACAAGCGTGTGACCAGATTCGTCCTCCCAGTCGGAGCAACCATTAACATGGATGGCAC AGCCCTTTACGAGGCTGTGGCAGCCATCTTCATAGCCCAAATGAATGGGGTCATCTTGGATGGAGGTCAG ATTGTGACTGTAAGCCTTACAGCCACCCTGGCGAGCATTGGTGCAGCCAGTATTCCCAGCGCCGGGCTGG TCACCATGCTCCTCATTCTCACAGCTGTGGGCCTGCCAACGGAGGATATCAGTCTGCTGGTGGCGGTGGA CTGGCTGCTGGATAGAATGAGAACTTCAGTCAATGTGGTGGGCGATTCTTTTGGGGCTGGGATTGTCTAT CACCTTTCCAAGTCTGAGCTGGACACCATTGACTCCCAACACCGAATGCAGGAAGACATCGAAATGACCA AGACGCAGTCCATTTACGACGACAAGAACCACAGGGAAAGCAACTCTAATCAGTGTGTCTATGCCGCACA CAACTCTGTCGTAATAGATGAGTGCAAGGTAACTCTGGCGGCCAATGGAAAGTCAGCTGACTGCAGTGTT GAGGAAGAACCTTGGAAACGTGAAAAATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001077515 |
| Insert Size | 1710 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001077515.2, NP_001070983.1 |
| RefSeq Size | 10998 bp |
| RefSeq ORF | 1710 bp |
| Locus ID | 20511 |
| UniProt ID | P43006 |
| Gene Summary | Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate (PubMed:7698742, PubMed:7557442, PubMed:9373176). Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion. Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport (By similarity). Essential for the rapid removal of released glutamate from the synaptic cleft, and for terminating the postsynaptic action of glutamate (PubMed:9180080).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG226163 | Slc1a2 (tGFP-tagged) - Mouse solute carrier family 1 (glial high affinity glutamate transporter) member 2 (Slc1a2) transcript variant 2, (10ug) |
CNY 5840.00 |
|
| MR226163 | Slc1a2 (Myc-DDK-tagged) - Mouse solute carrier family 1 (glial high affinity glutamate transporter), member 2 (Slc1a2), transcript variant 2 |
CNY 4240.00 |
|
| MR226163L3 | Lenti ORF clone of Slc1a2 (Myc-DDK-tagged) - Mouse solute carrier family 1 (glial high affinity glutamate transporter), member 2 (Slc1a2), transcript variant 2 |
CNY 6940.00 |
|
| MR226163L4 | Lenti ORF clone of Slc1a2 (mGFP-tagged) - Mouse solute carrier family 1 (glial high affinity glutamate transporter), member 2 (Slc1a2), transcript variant 2 |
CNY 6940.00 |
