Lpcat2 (NM_173014) Mouse Untagged Clone
CAT＃: MC218670
Lpcat2 (untagged) - Mouse lysophosphatidylcholine acyltransferase 2 (Lpcat2), (10ug)
CNY 6940.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | A330042H22; Aytl1; Aytl1a; lpafat1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC218670 representing NM_173014
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACCGATGCGCCGAGGCGGCCGCTGTGGCGGCTACGGTCCCGGGTTCGGGCGTCGGGGACGCCGGGC TGCGGCCACCCATGGTACCTCGCCAGGCGTCCTTCTTCCCGCCGCCGGTGCCCAACCCCTTCGTCCAGCA GACTACGATCAGTGCCTCCCGAAGGCTGCAGATGTTTCTTCTTGGAATTATTCTGCTCCCAGTCCGTGCT TTACTGGTTGGAATAATTTTGCTGCTCGCATGGCCATTTGCTGTCATTTCAACAGCATGCTGTCCTGAAA AGCTGACCCATCCAATAAGCAATTGGAGGAGGAAGATCACTCGGCCCGCTTTGACATTTCTGGCGCGTGC CATGTTCTTCTCCATGGGGTTTACGGTTACCGTGAAAGGAAAGGTTGCAAGCCCTCTGGAAGCGCCCATT TTTGTCGTCGCTCCTCATTCCACGTTCTTCGACGGAATTGCCTGTGTAGTAGCTGGGCTGCCTTCGCTGG TATCACGGAATGAGAATGCACAGACCCCTCTGGTTGGCAGACTGTTACGGGCTTTGCAGCCAGTTTTGGT ATCCCGTGTTGACCCTGACTCCCGAAAAAACACAATCAATGAAATCAAAAAGCGAGCGACATCAGGTGGA GAATGGCCCCAGATACTAGTTTTCCCAGAAGGTACTTGTACTAATCGCTCCTGTTTGATTACTTTTAAAC CAGGAGCCTTCATCCCAGGAGTTCCAGTGCAGCCCGTCCTCCTCAGATACCCAAACAAGTTGGATACTGT GACCTGGACCTGGCAAGGCTATACATTCCTCCAGCTGTGTGTGCTCACTTTCTGTCAACTCTTCACGAAG GTGGAGATTGAGTTTATGCCTGTGCAAGCACCCAGTGAGGAAGAGAAGAACGATCCTGTCCTTTTCGCCA GTAGAATCCGGAACTTAATGGCTGAAGCATTGGAAATACCCGTGACGGATCACACCTACGAAGACTGCAG GCTGATGATCTCAGCGGGACAGCTCACGTTGCCTATGGAGGCCGGGCTGGTAGAATTTTCAAAGATTAGC CGGAAACTGAAATTAGACTGGGATGGTATCCGGAAGCACTTGGATGAATATGCGTCTATCGCAAGCTCTT CCAAAGGAGGCAGAATTGGAATCGAGGAGTTTGCGGAATACTTGAAGCTACCTGTTTCCGATGTCCTGAG ACAACTTTTTGCACTCTTTGACAGGAATAATGATGGCAGTATTGACTTCCGAGAGTATGTGATCGGCCTG GCTGTCTTGTGCAACCCTGCCAATACAGAAGAGATCATCCAGGTGGCATTTAAGCTCTTTGATGTTGATG AGGACGGCTACATAACAGAGGAGGAGTTTTGCACCATTCTGCAGGCTTCTCTTGGAGTGCCTGACCTTAA TGTTTCTGGTCTCTTCAGAGAAATTGCCCAACGGGACTCTGTTTCCTACGAGGAATTCAAAAGTTTCGCC CTAAAGCACCCAGAATATGCCAAGATATTTACCACATACCTAGACCTCCAGACATGCCATGTATTCTCCT TGCCAGAAGAGGTCCAGACAGCTCCCTCTGTTGCTAGTAATAAAGTCAGTCCTGAGAGCCAGGAGGAGGG CACCTCAGACAAAAAGGTGGACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_173014 |
| Insert Size | 1635 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_173014.1, NP_766602.1 |
| RefSeq Size | 2800 bp |
| RefSeq ORF | 1635 bp |
| Locus ID | 270084 |
| UniProt ID | Q8BYI6 |
| Gene Summary | Possesses both acyltransferase and acetyltransferase activities (PubMed:17182612, PubMed:18156367). Activity is calcium-dependent (PubMed:17182612). Involved in platelet-activating factor (PAF) biosynthesis by catalyzing the conversion of the PAF precursor, 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF) into 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (PAF) (PubMed:17182612). Also converts lyso-PAF to 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine (PC), a major component of cell membranes and a PAF precursor (PubMed:17182612, PubMed:18156367). Under resting conditions, acyltransferase activity is preferred (PubMed:17182612). Upon acute inflammatory stimulus, acetyltransferase activity is enhanced and PAF synthesis increases (PubMed:17182612). Also catalyzes the conversion of 1-acyl-sn-glycero-3-phosphocholine to 1,2-diacyl-sn-glycero-3-phosphocholine. Involved in the regulation of lipid droplet number and size (By similarity).[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG218203 | Lpcat2 (tGFP-tagged) - Mouse lysophosphatidylcholine acyltransferase 2 (Lpcat2), (10ug) |
CNY 5990.00 |
|
| MR218203 | Lpcat2 (Myc-DDK-tagged) - Mouse lysophosphatidylcholine acyltransferase 2 (Lpcat2) |
CNY 6072.00 |
|
| MR218203L3 | Lenti ORF clone of Lpcat2 (Myc-DDK-tagged) - Mouse lysophosphatidylcholine acyltransferase 2 (Lpcat2) |
CNY 7320.00 |
|
| MR218203L4 | Lenti ORF clone of Lpcat2 (mGFP-tagged) - Mouse lysophosphatidylcholine acyltransferase 2 (Lpcat2) |
CNY 7320.00 |
