Sars (NM_011319) Mouse Untagged Clone
CAT＃: MC217577
Sars (untagged) - Mouse seryl-aminoacyl-tRNA synthetase (Sars), transcript variant 1, (10ug)
CNY 6840.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | Sars1; serRS; Strs |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC217577 representing NM_011319
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGCTGGACCTGGATTTGTTTCGGGTGGATAAAGGAGGGGACCCAGCCCTCATTCGAGAGACGCAGG AGAAGCGCTTCAAGGACCCGGGGCTGGTGGACCAGCTGGTGAAAGCAGACAGTGAGTGGCGACGATGCAG ATTTCGGGCAGACAACTTGAACAAGCTGAAGAATTTATGCAGCAAAACTATTGGGGAGAAAATGAAGAAA AAGGAAGCAGTGGGAGACGACGAGTCCGTCCCAGAGAACGTGCTGAATTTCGATGACCTCACTGCAGACG CGCTAGCTGCCCTGAAAGTCTCACAGATTAAAAAAGTCCGACTCCTCATTGATGAAGCCATCCAGAAGTG TGATGGGGAGCGGGTAAAGCTGGAAGCAGAGCGATTTGAGAACCTCCGCGAGATTGGGAACCTTCTGCAC CCCTCTGTGCCCATTAGTAATGATGAGGACGCAGACAACAAAGTAGAACGTATTTGGGGAGATTGTACAG TCAGGAAGAAGTATTCCCATGTGGACCTGGTGGTGATGGTAGATGGCTTTGAAGGCGAAAAGGGAGCCGT GGTGGCTGGTAGTCGGGGGTACTTCCTGAAGGGGCCCCTGGTGTTCCTGGAGCAGGCGCTTATCCAATAT GCACTGCGTACCTTGGGGAGTCGGGGCTACACTCCAATCTACACCCCCTTCTTCATGAGGAAAGAGGTCA TGCAGGAAGTGGCCCAGCTCAGCCAGTTTGATGAAGAACTTTATAAGGTGATTGGCAAAGGCAGCGAAAA GTCAGATGACAACTCCTATGACGAGAAATACTTGATTGCCACCTCAGAGCAGCCCATCGCGGCTCTGCAC CGGGACGAGTGGCTGCGGCCAGAGGATCTGCCCATCAAGTACGCTGGCCTCTCCACCTGCTTTCGTCAGG AAGTGGGCTCGCATGGCCGTGACACCCGTGGTATCTTCCGAGTCCATCAGTTTGAGAAGATTGAGCAGTT TGTGTACTCATCGCCCCATGACAATAAGTCGTGGGAGATGTTTGATGAGATGATCGCCACCGCAGAAGAA TTCTACCAGTCTTTGGGGATCCCTTACCACATTGTGAATATTGTCTCAGGCTCCTTGAATCACGCTGCCA GTAAGAAGCTCGACCTGGAGGCCTGGTTCCCAGGCTCGGGTGCCTTCCGTGAGTTGGTGTCCTGTTCTAA TTGCACGGATTACCAAGCTCGCCGCCTGAGAATCCGATATGGGCAGACCAAGAAGATGATGGACAAGAAA AAGAATAATCTCCGTTTCTCTACACAGAACAAGTTGAAGGCAATGTCTTCCCTCTTTTCGCCCAAGAAGG TGGAGTTTGTCCATATGCTTAATGCTACAATGTGTGCTACCACCCGGACCATCTGCGCCATCCTGGAGAA CTACCAGGCAGAGAAGGGCATCGCTGTGCCAGAGAAGTTGAGGGAGTTCATGCCGCCAGGGCTCCAAGAG CTGATCCCGTTTGTGAAGCCTGCACCCATTGACCAGGAGCCATCTAAGAAGCAGAAGAAGCAACATGAAG GCAGCAAAAAGAAAGCGAAAGAGGTCCCCCTGGAGAACCAGCTGCAGAGCATGGAGGTCACTGAGGCCTG A ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_011319 |
| Insert Size | 1611 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_011319.3, NP_035449.1 |
| RefSeq Size | 3704 bp |
| RefSeq ORF | 1611 bp |
| Locus ID | 20226 |
| Gene Summary | Catalyzes the attachment of serine to tRNA(Ser) in a two-step reaction: serine is first activated by ATP to form Ser-AMP and then transferred to the acceptor end of tRNA(Ser). Is probably also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec). In the nucleus, binds to the VEGFA core promoter and prevents MYC binding and transcriptional activation by MYC. Recruits SIRT2 to the VEGFA promoter, promoting deacetylation of histone H4 at 'Lys-16' (H4K16). Thereby, inhibits the production of VEGFA and sprouting angiogenesis mediated by VEGFA.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG220183 | Sars (tGFP-tagged) - Mouse seryl-aminoacyl-tRNA synthetase (Sars), (10ug) |
CNY 5040.00 |
|
| MR220183 | Sars (Myc-DDK-tagged) - Mouse seryl-aminoacyl-tRNA synthetase (Sars), transcript variant 1 |
CNY 3992.00 |
|
| MR220183L3 | Lenti ORF clone of Sars (Myc-DDK-tagged) - Mouse seryl-aminoacyl-tRNA synthetase (Sars), transcript variant 1 |
CNY 6460.00 |
|
| MR220183L4 | Lenti ORF clone of Sars (mGFP-tagged) - Mouse seryl-aminoacyl-tRNA synthetase (Sars), transcript variant 1 |
CNY 6460.00 |
