Rpe65 (NM_029987) Mouse Untagged Clone
CAT＃: MC217576
Rpe65 (untagged) - Mouse retinal pigment epithelium 65 (Rpe65), (10ug)
CNY 4376.00
CNY 6840.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 65kDa; A930029L06Rik; LCA2; Mord1; rd12; RP20 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC217576 representing NM_029987
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTATCCAAATTGAACACCCTGCTGGTGGCTACAAGAAACTATTTGAAACTGTGGAGGAACTGTCCT CACCACTAACAGCTCATGTCACAGGCAGGATTCCCCTCTGGCTCACTGGCAGTCTCCTCCGATGTGGGCC AGGGCTCTTTGAAGTTGGATCTGAGCCTTTCTATCACCTGTTTGATGGACAAGCCCTTTTGCACAAGTTT GACTTCAAGGAGGGCCATGTCACATACCACAGAAGATTCATCCGCACTGATGCTTATGTTCGAGCAATGA CTGAGAAGAGGATTGTCATAACAGAATTTGGCACCTGTGCTTTCCCAGACCCCTGCAAGAATATATTTTC CAGGTTTTTTTCTTACTTTAAAGGAGTAGAGGTTACTGACAATGCCCTTGTAAATATCTACCCAGTGGGA GAAGATTACTATGCATGCACAGAGACCAACTTTATCACAAAGATTAACCCAGAGACCTTGGAGACAATTA AGCAGGTTGATCTTTGCAACTATATTTCTGTCAATGGTGCCACTGCTCATCCACATATTGAAAGTGATGG AACAGTTTACAACATTGGGAATTGCTTTGGAAAAAATTTTACAGTTGCCTACAACATTATTAAGATCCCT CCACTGAAAGCAGACAAGGAAGATCCAATAAACAAGTCAGAAGTTGTTGTGCAGTTCCCCTGCAGTGATC GTTTCAAGCCATCTTATGTACACAGTTTTGGTCTGACTCCCAACTATATCGTTTTTGTGGAGACTCCAGT CAAAATTAACCTTTTCAAGTTTCTTTCTTCGTGGAGTCTTTGGGGAGCCAACTACATGGACTGTTTCGAG TCCAATGAAAGCATGGGGGTTTGGCTTCATGTTGCTGATAAAAAAAGAAGAAAATACTTCAATAACAAAT ACAGGACTTCCCCTTTCAATCTCTTCCATCATATCAATACTTATGAAGACAATGGATTTCTGATTGTGGA TCTCTGTTGCTGGAAAGGGTTTGAATTTGTTTATAATTACTTATATTTAGCCAATTTACGTGAGAATTGG GAAGAAGTTAAAAGAAATGCTATGAAGGCTCCTCAGCCTGAAGTCAGGAGATATGTACTTCCTTTGACAA TTGACAAGGTCGACACAGGCAGAAATTTAGTCACACTGCCCCATACAACTGCCACAGCCACTCTGCGCAG TGATGAGACCATATGGCTGGAACCTGAGGTTCTCTTTTCAGGGCCTCGTCAAGCCTTTGAATTTCCTCAA ATCAATTACCAGAAATTTGGAGGGAAACCTTATACTTATGCATACGGACTTGGGTTGAATCACTTTGTTC CTGACAAGCTCTGTAAGATGAACGTCAAAACTAAAGAAATCTGGATGTGGCAAGAGCCAGATTCTTACCC ATCTGAACCCATCTTTGTTTCTCAACCAGATGCTCTGGAAGAAGATGATGGTGTGGTTCTGAGTGTGGTG GTGAGCCCTGGGGCAGGGCAAAAGCCTGCATATCTCCTGGTTCTGAATGCCAAAGACTTGAGTGAAATTG CCAGGGCTGAAGTGGAGACTAATATCCCTGTGACCTTCCATGGACTGTTCAAAAGATCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_029987 |
| Insert Size | 1602 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_029987.2, NP_084263.2 |
| RefSeq Size | 1862 bp |
| RefSeq ORF | 1602 bp |
| Locus ID | 19892 |
| UniProt ID | Q91ZQ5 |
| Gene Summary | Critical isomerohydrolase in the retinoid cycle involved in regeneration of 11-cis-retinal, the chromophore of rod and cone opsins. Catalyzes the cleavage and isomerization of all-trans-retinyl fatty acid esters to 11-cis-retinol which is further oxidized by 11-cis retinol dehydrogenase to 11-cis-retinal for use as visual chromophore (PubMed:15765048, PubMed:9843205, PubMed:23407971, PubMed:28500718). Essential for the production of 11-cis retinal for both rod and cone photoreceptors (PubMed:17251447). Also capable of catalyzing the isomerization of lutein to meso-zeaxanthin an eye-specific carotenoid. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT (By similarity).[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG223598 | Rpe65 (tGFP-tagged) - Mouse retinal pigment epithelium 65 (Rpe65), (10ug) |
CNY 7552.00 |
|
| MR223598 | Rpe65 (Myc-DDK-tagged) - Mouse retinal pigment epithelium 65 (Rpe65) |
CNY 5952.00 |
|
| MR223598L3 | Lenti ORF clone of Rpe65 (Myc-DDK-tagged) - Mouse retinal pigment epithelium 65 (Rpe65) |
CNY 6560.00 |
|
| MR223598L4 | Lenti ORF clone of Rpe65 (mGFP-tagged) - Mouse retinal pigment epithelium 65 (Rpe65) |
CNY 6560.00 |
