Rcc2 (NM_173867) Mouse Untagged Clone
CAT＃: MC217502
Rcc2 (untagged) - Mouse regulator of chromosome condensation 2 (Rcc2), (10ug)
CNY 6750.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 2610510H01Rik; 2610529N02Rik; AA536646; AA675016; mKIAA1470; Td60 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC217502 representing NM_173867
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCAGGAAGAAGGGGGCGGCCTGGGAGGAGCCGAGTTCGGGCAACGGCACGGCGCGCGCGGGGCCCC GGCGACGCGGCGGCCCCGCGGGCAGGAAGCGCGAGCGGCCCGAGCGCTGCAGCAGCAGCAGCGGCGGCGG CAGCAGCGGCGACGAGGACGGCCCGGAGCTGGACGGGGCCCCCGGCGGCGGCAAGCGCACAGCCAGGCCG GCGACGGCGGGCAAGGCGGCCGGCGCGGCCGCGATCATCACGGAGCCCGAGCACACGAAGGAGCGCGTCA AACTTGAAGGGTCTAAGTGCAAAGGGCAGCTTTTGATTTTTGGGGCAACCAACTGGGACTTGATTGGACG GAAGGAAGTGCCTAAACAGCAAGCTGCGTACCGCAACCTTGGTCAGAATTTGTGGGGGCCCCATAGGTAT GGGTGCCTGTCAGGAGTCCGGGTGCGAACCGTGGTTTCAGGCTCATGTGCTGCCCACAGCCTCCTCATCA CCACAGAAGGGAAGCTGTGGAGCTGGGGTCGGAATGAGAAGGGGCAGCTGGGCCATGGTGATACCAAGAG GGTGGAAGCCCCCAGACTCATCGAGGCCCTCAGCCATGAGGCAATCGTGCTGGCAGCCTGTGGGCGCAAC CACACCCTGGCCTTGACAGATACTGGATCAGTGTTTGCCTTCGGAGAGAATAAAATGGGGCAGCTGGGTC TCGGGAACCAGACAGATGCCGTCCCAAGTCCTGCTCAGATAATGTACAACGGGCAGCCAATCACCAAGAT GGCCTGCGGGGCCGAGTTCAGCATGCTGATGGACTGTAAAGGAAACCTCTACTCCTTTGGGTGCCCTGAG TATGGCCAGCTGGGGCACAACTCAGATGGCAAGTTCATCGCCCGGGCGCAGAGGATAGAATATGATTGTG AGCTGGTGCCCCGCAGAGTGGCCATCTTCATCGAGAAGACCAAGGACGGGCAGATCTTACCTGTCCCCAA CGTGGTGGTTCGAGATGTAGCCTGTGGCGCTAACCACACACTGGTCCTGGACTCTCAGAAGCGAGTGTTC TCCTGGGGCTTTGGTGGCTATGGCCGGCTGGGCCATGCCGAGCAGAAGGATGAGATGGTGCCTCGCCTGG TGAAGCTGTTCGACTTCCCGGGGCGTGGTGCAACCCAGATCTATGCCGGCTACACCTGCTCCTTCGCTGT CAGTGAAGTGGGTGGCCTGTTTTTCTGGGGGGCCACCAATACCTCCCGAGAGTCTACCATGTACCCGAAG GCAGTGCAGGACCTCTGCGGCTGGCGGATCCGGAGCCTTGCCTGCGGGAAGAGCAGCATCATCGTAGCTG CGGACGAGAGTACTATCAGTTGGGGTCCATCGCCAACCTTCGGGGAATTGGGGTATGGGGACCACAAGCC CAAGTCTTCTACTGCGGCCCAGGAGGTGAAGACTCTGGATGGCATCTTCTCCGAACAGGTGGCCATGGGC TATTCACACTCCTTGGTGATAGCGCGAGACGAGAGCGAGGCTGAGAAGGAGAAGCTGCAGAGGCTACCTG AGTACACCCCGCGCACCCTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_173867 |
| Insert Size | 1563 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_173867.5, NP_776292.1 |
| RefSeq Size | 3790 bp |
| RefSeq ORF | 1563 bp |
| Locus ID | 108911 |
| UniProt ID | Q8BK67 |
| Gene Summary | Multifunctional protein that may effect its functions by regulating the activity of small GTPases, such as RAC1 and RALA. Required for normal progress through the cell cycle, both during interphase and during mitosis. Required for the presence of normal levels of MAD2L1, AURKB and BIRC5 on inner centromeres during mitosis, and for normal attachment of kinetochores to mitotic spindles. Required for normal organization of the microtubule cytoskeleton in interphase cells. Functions as guanine nucleotide exchange factor (GEF) for RALA. Interferes with the activation of RAC1 by guanine nucleotide exchange factors (By similarity). Prevents accumulation of active, GTP-bound RAC1, and suppresses RAC1-mediated reorganization of the actin cytoskeleton and formation of membrane protrusions (PubMed:25074804). Required for normal cellular responses to contacts with the extracellular matrix of adjacent cells, and for directional cell migration in response to a fibronectin gradient (in vitro) (By similarity).[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR208331 | Rcc2 (Myc-DDK-tagged) - Mouse regulator of chromosome condensation 2 (Rcc2) |
CNY 3872.00 |
|
| MR208331L3 | Lenti ORF clone of Rcc2 (Myc-DDK-tagged) - Mouse regulator of chromosome condensation 2 (Rcc2) |
CNY 6460.00 |
|
| MR208331L4 | Lenti ORF clone of Rcc2 (mGFP-tagged) - Mouse regulator of chromosome condensation 2 (Rcc2) |
CNY 6460.00 |
