Pi4k2b (NM_025951) Mouse Untagged Clone
CAT＃: MC216317
Pi4k2b (untagged) - Mouse phosphatidylinositol 4-kinase type 2 beta (Pi4k2b), transcript variant 1, (10ug)
CNY 6080.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 2610042N09Rik; 4933409G22Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC216317 representing NM_025951
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGGCCTGCGAGCCCACCCGCCCCTCGGAGGACGAGGACGAGGAGCGGGAGCCGCTGCTGCCTC GCGTTGCCTGGGCCCAGCCGCGGAGGGTCGCGCCCGGGAGCGCCGTGAGAATGCAGGCGGACGAGGGCGC GGATGTCCTCCGCGAGCCCGCTACCGACGAGCCGCCGGCGGTGTCCGGGGAAGGGTCGATCTCCGCGAGC TTGTCCACCGAGCTGGATCGGACCCGCACTACGAGCTCAGAAACAAATACATTCTTGGAAGATCCAGAAT TTGCTGATATTGTATTGAAAGCAGAGCAAGCAATAGAAATTGGAGTTTTCCCAGAAAGAATTTCTCAAGG GTCAAGTGGAAGTTACTTTGTAAAGGATTCTAAAAGGAATATTATTGGCGTGTTTAAACCCAAATCAGAA GAGCCTTATGGCCAGCTGAACCCCAAGTGGACCAAGTACGTCCATAAGGTTTGCTGTCCCTGCTGCTTTG GCCGAGGCTGCCTGCTTCCTAACCAGGGGTACCTTTCTGAAGCTGGTGCCTACCTGGTGGATGTCAAACT TAATCTGGGTATTGTGCCTAAAACAAAGGTGGTTTGGCTTGTCAGTGAGACATTTAACTACAGTGCAATT GACCGTGCAAAATCAAGAGGCAAAAAGTATGCCTTAGAGAAAGTGCCAAAAGTAGGCAGAAAGTTCCATC GGATAGGACTTCCTCCTAAGGTTGGTTCCTTTCAGCTGTTTGTGAAAGATTACAAGGAGGCTGAGTATTG GCTTAGGAGGTTTGAAGCGGAGCCTCTGCCCGAGAACATTAGAAAACAGTTTCAGTCACAGTTTGAAAAA TTAGTGATTCTGGATTACATCATCAGGAACACAGACAGGGGAAATGATAACTGGCTAGTCAAATATGATG AGATGAAGTATGCAAAGAAAATTGAAAGTGAGGAATCAAACTGGATTGATAATAAACAATTGCTTATTAA AATAGCTGCAATTGATAATGGGCTAGCATTTCCCTTTAAGCATCCTGATGAATGGCGAGCATATCCATTT CACTGGGCTTGGCTTCCTCAAGCAAAGGTTCCTTTTTCTGAAGAGACTCGAAACTTGATTCTACCCTATA TTTCTGACATGAACTTTGTACAAGACTTGTGTGAAGATCTTTATGAACTTTTTAAGACTGACAAAGGATT TGACAGAGCTGCTTTTGAAAATCAGATGTCTGTGATGAGAGGCCAGATCTTAAACCTCACTCAGGCACTG AGAGACGGGAAGAGTCCTATGCAGCTGGCACAGATGCCATGTGTGATCGTGGAGTGCAGCAAAAGTGGCA GCCAGGGCCGTGTGGTACACCTGGGCAGCTCCTTCACCCAGACGGTCCACTGCAGGAAACCGTTCTTCTC CTCCTGGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_025951 |
| Insert Size | 1410 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_025951.3, NP_080227.2 |
| RefSeq Size | 3143 bp |
| RefSeq ORF | 1410 bp |
| Locus ID | 67073 |
| UniProt ID | Q8CBQ5 |
| Gene Summary | Together with PI4K2A and the type III PI4Ks (PIK4CA and PIK4CB) it contributes to the overall PI4-kinase activity of the cell. This contribution may be especially significant in plasma membrane, endosomal and Golgi compartments. The phosphorylation of phosphatidylinositol (PI) to PI4P is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3). Contributes to the production of InsP3 in stimulated cells and is likely to be involved in the regulation of vesicular trafficking (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG207499 | Pi4k2b (tGFP-tagged) - Mouse phosphatidylinositol 4-kinase type 2 beta (Pi4k2b), transcript variant 1 |
CNY 4470.00 |
|
| MR207499 | Pi4k2b (Myc-DDK-tagged) - Mouse phosphatidylinositol 4-kinase type 2 beta (Pi4k2b), transcript variant 1 |
CNY 3656.00 |
|
| MR207499L3 | Lenti ORF clone of Pi4k2b (Myc-DDK-tagged) - Mouse phosphatidylinositol 4-kinase type 2 beta (Pi4k2b), transcript variant 1 |
CNY 5990.00 |
|
| MR207499L4 | Lenti ORF clone of Pi4k2b (mGFP-tagged) - Mouse phosphatidylinositol 4-kinase type 2 beta (Pi4k2b), transcript variant 1 |
CNY 5990.00 |
