Mocs3 (NM_001160330) Mouse Untagged Clone
CAT＃: MC216156
Mocs3 (untagged) - Mouse molybdenum cofactor synthesis 3 (Mocs3), (10ug)
CNY 5990.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 1700020H17Rik; Uba4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC216156 representing NM_001160330
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCCCCGGAGGACGTAGCTGCCTTACAGGCTGAAATCACCCGGCGGGAGGAAGAGCTAGCTTCGC TCAAGCGGAGGCTGGCCGCGGCCCTGACAGCCGAGCCGGAGCCCGAGCGTCCGCTCCGGGTCCCGCCGCC GCCGCTGGCGCCCCGGGCCGCGCTGTCGCGGGACGAGATCCTCCGCTACAGCCGCCAGCTACTGCTGCCG GAGCTGGGCGTGCGCGGGCAGCTGCGCCTGGCGGCGGCGGCGGTGCTCGTGGTGGGCTGCGGCGGGCTGG GCTGCCCGCTGGCGCAGTACCTGGCGGCGGCCGGCGTAGGCAGGCTGGGGCTGGTGGACCACGACGTGGT GGAGACGAGCAACCTGGCCCGCCAGGTCCTGCACGGGGAGGCGCAGGCCGGCGAGAGCAAGGCTCGCTCG GCGGCCGCGGCGCTGCGCCGTCTCAACTCGGCGGTGGAGTGCGTGGCGTACCCGCGCGCGCTCGCCGAGG ACTGGGCGCTCGACCTGGTCCGCGGCTACGACGTGGTGGCAGACTGCTGCGACAACGTGCCCACGCGCTA CCTGGTGAACGACGCGTGCGTGCTGGCCGGCCGGCCGCTAGTGTCGGCCAGCGCGCTGCGCTTCGAGGGC CAGATGACCGTCTACCACCACGACGGCGGGCCGTGCTACCGCTGCGTGTTCCCGCGGCCGCCCCCGCCGG AGACAGTGACCAACTGTGCAGACGGCGGCGTGCTCGGAGCGGTGCCCGGCGTGCTAGGCTGCGCGCAGGC CCTCGAGGTGCTCAAGATCGCCGCCGGCCTCGGCTCCTCCTACAGCGGCAGCATGCTGCTCTTCGACGGC CTCGGGGGCCACTTCCGCCGGATCCGGCTGCGGCGCCGCCGGCCTGACTGCGTCGTGTGCGGGCAGCAGC CCACCGTGACCCGCCTGCAGGACTACGAGGCCTTCTGCGGCTCCTCGGCCACCGACAAGTGCCGCGCCCT GAAGCTGCTGTGCCCCGAGGAGCGGATTTCTGTGACCGACTACAAGCGGCTTCTGGATTCCGGGGCGCCC CATGTGTTGCTGGACGTCCGGCCTCAAGTAGAGGTGGACATCTGTCGCCTGCCGCACTCTCTCCACATCC CTTTGAGTCAGTTGGAACGCAGGGATGCGGACAGCCTGAAACTCTTAGGGGCTGCCCTCCGGAAAGGGAA GCAGGAGTCGCAGGAAGGGGTTGCTCTCCCGGTGTATGTGATTTGCAAACTGGGAAACGACTCCCAGAAA GCTGTGAAGGTCCTGCAGTCCTTAACGGCAGTGCCGGAGTTAGACTCTTTAACTGTTCAGGACATCGTGG GGGGACTCATGGCCTGGGCCGCCAAAATTGATGGGACATTTCCACAGTACTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-RsrII |
| ACCN | NM_001160330 |
| Insert Size | 1383 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001160330.1, NP_001153802.1 |
| RefSeq Size | 1691 bp |
| RefSeq ORF | 1383 bp |
| Locus ID | 69372 |
| UniProt ID | A2BDX3 |
| Gene Summary | Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions.[UniProtKB/Swiss-Prot Function] |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG218056 | Mocs3 (tGFP-tagged) - Mouse molybdenum cofactor synthesis 3 (Mocs3), (10ug) |
CNY 3710.00 |
|
| MR218056 | Mocs3 (Myc-DDK-tagged) - Mouse molybdenum cofactor synthesis 3 (Mocs3) |
CNY 3656.00 |
|
| MR218056L3 | Lenti ORF clone of Mocs3 (Myc-DDK-tagged) - Mouse molybdenum cofactor synthesis 3 (Mocs3) |
CNY 5230.00 |
|
| MR218056L4 | Lenti ORF clone of Mocs3 (mGFP-tagged) - Mouse molybdenum cofactor synthesis 3 (Mocs3) |
CNY 5230.00 |
