Ccdc84 (NM_201372) Mouse Untagged Clone
CAT＃: MC214748
Ccdc84 (untagged) - Mouse coiled-coil domain containing 84 (Ccdc84), (10ug)
CNY 3990.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | D630044F24Rik; Gm1114 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC214748 representing NM_201372
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCCACCGCAGCGATGTCCTCTGTGTCGGCAAACTTTCTTCTGTGGTCGCGGTCACGTCTACAGCC ACAAGCACCAGCGGCAGTTGAAGGGGGCTCTGGAGCGGCTTCTACCGCAGGTGGAGGCCGCCCGCCGGGC CGTCCGCGCCGCCCAGGTGGAGCGCTATGTCCCCGAGCACGATCGGTGCTGTTGGTGCCCGTGCTGTGGC TGCGAAGTGCGAAAACACCTGAGCCATGGCAACCTGACAGTGCTACACGGGGGTCTGCTGGAACATCTGG CCAGCCCAGAGCACAAGAAAGCCACCAACAAATTCTGGTGGGAGAACAAGGCCAATGCCCAGATGAAAGA GAAGTTTCTGATCTCCCCCCAGGATTATGCACGGTTTAAGAAGTCCATGGTGAAGGGCTTGGATTCCTAT GAGGAAAAGGAGGACGAGGTGATTAAAGAGATGGCAGCTCAGATCCGTGAGGTAGAACAGAGTCGGCAGG AAGTGGTCCGATCTGTCTTAGAGCCTCAGGCAGAGTCAGATCCAGAAGAGGGCTCTTCAGCACCTGAAAG TTGGAAAGCGACCAACGGCCATGTAGCCTCCAGCTCACAGCAGGTATCACACTTGGCCTTGCAACCTGTT GCAGAGCTTGATTGGATGGAGACAGGACAGCAACTAACATTCATTGGCCATCAGGATACACCCGGAATTG GTAACATTCACTCAGGTGCCACACCTCCCTGGATGATCCAAGAGGAGGAGCACAGCTCTGGCAGCCTCCC AATAGGACCCTCCTATGAGGAATTTCTCAAAGAAAAGGAAAAACAAAAACTGAAGAAACTCCCTCCAGAT AGAGTTGGCGCCAACTTTGACCACAGTTCCAACACCAGTGCAGGCTGGTTGCCTTCTTTTGGCAGAGTCT GGAATAATGGACGCCGCTGGCAGTCCAGGCATCAATTCAAAACCGAAGCTGCAACAAGAAGCAAGCAACC ACGTAAAGGGAAAAGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_201372 |
| Insert Size | 999 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_201372.3, NP_958760.2 |
| RefSeq Size | 1175 bp |
| RefSeq ORF | 999 bp |
| Locus ID | 382073 |
| UniProt ID | Q4VA36 |
| Gene Summary | Negative regulator of centrosome duplication. Constrains centriole number by modulating the degradation of the centrosome-duplication-associated protein SASS6 in an acetylation-dependent manner. SIRT1 deacetylates CENATAC in G1 phase, allowing for SASS6 accumulation on the centrosome and subsequent procentriole assembly. The CENATAC acetylation level is restored in mitosis by NAT10, promoting SASS6 proteasome degradation by facilitating SASS6 binding to APC/C E3 ubiquitin-protein ligase complex/FZR1.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the protein-coding transcript. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG204904 | Ccdc84 (tGFP-tagged) - Mouse coiled-coil domain containing 84 (Ccdc84) |
CNY 2850.00 |
|
| MR204904 | Ccdc84 (Myc-DDK-tagged) - Mouse coiled-coil domain containing 84 (Ccdc84) |
CNY 2400.00 |
|
| MR204904L3 | Lenti ORF clone of Ccdc84 (Myc-DDK-tagged) - Mouse coiled-coil domain containing 84 (Ccdc84) |
CNY 4750.00 |
|
| MR204904L4 | Lenti ORF clone of Ccdc84 (mGFP-tagged) - Mouse coiled-coil domain containing 84 (Ccdc84) |
CNY 4750.00 |
