Alkbh5 (NM_172943) Mouse Untagged Clone
CAT＃: MC214261
Alkbh5 (untagged) - Mouse alkB, alkylation repair homolog 5 (E. coli) (Alkbh5), (10ug)
CNY 3990.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | Abh5; AW050020; AW215868; E130207K11; Ofoxd |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC214261 representing NM_172943
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCCGCCAGCGGCTACACCGACCTGCGGGAGAAGCTCAAGTCCATGACGTCCCGGGACAACTACA AGGCGGGCAGTCGGGAGGCCGCCGCCGCTGCGGCCGCCGCCGTGGCTGCCGCTGCCGCTGCCGCGGCCGC CGCTGAGCCTTACCCGGCGTCCGGGACCACCAAGCGGAAATACCAGGAGGACTCGGACCCCGAGCGCAGC GACTACGAGGAGCATCAGTTGCAGAAGGAGGAAGAGGCGCGCAAGGTGAAGAGCGGCATCCGGCAGATCC GGCTCTTCAGTCAGGATGAGTGCTCCAAGATCGAGGCCCGCATCGATGAGGTGGTGTCCCGCGCCGAGAA GGGCCTGTACAACGAGCACACGGTGGACCGGGCCCCCCTGCGCAACAAGTACTTCTTCGGCGAGGGCTAC ACGTACGGGGCCCAGCTGCAGAAGCGCGGGCCGGGCCAGGAGCGCCTCTACCCGCCGGGCGACGTCGACG AGATCCCGGACTGGGTGCATCAGCTGGTGATCCAGAAGCTGGTGGAGCACCGCGTCATCCCCGAGGGCTT CGTCAACAGCGCGGTCATCAACGACTACCAGCCCGGCGGCTGCATCGTGTCCCACGTTGACCCCATCCAC ATCTTCGAGCGCCCCATCGTGTCCGTGTCTTTCTTCAGCGACTCGGCACTTTGCTTCGGCTGCAAGTTCC AGTTCAAGCCCATCCGGGTGTCGGAACCTGTGCTTTCTCTGCCGGTGCGCAGGGGGAGCGTGACTGTGCT CAGTGGGTATGCTGCTGATGAAATCACTCACTGCATACGGCCTCAGGACATTAAGGAACGCCGGGCGGTC ATCATTCTCAGGAAGACAAGATTAGATGCACCGCGATTGGAAACAAAATCCCTGAGCAGCTCCACATTGC CACCCAGCTATGCTTCAGATCGCCTGTCAGGAAACACCAGAGACCCTGCGCTGAAACCCAAAAGGTCCCA CCGCAAGGCAGACCCTGATGCTGCCCACAGGCCCCGGATCCTGGAAATGGACAAAGAAGAAAACCGGCGG TCTGTGCTCCTGCCCACACACCGGCGGAGGGGGAGTTTTAGCTCTGAGAACTATTGGCGCAAATCCTATG AGTCCTCGGAAGATTGCCCAGAGGCAGCCAGCAGCCCCACCCGCAAGGTGAAGATGAGGAGACACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_172943 |
| Insert Size | 1188 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_172943.4, NP_766531.2 |
| RefSeq Size | 6025 bp |
| RefSeq ORF | 1188 bp |
| Locus ID | 268420 |
| UniProt ID | Q3TSG4 |
| Gene Summary | Dioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes (PubMed:29279410). Can also demethylate N(6)-methyladenosine in single-stranded DNA (in vitro) (By similarity). Requires molecular oxygen, alpha-ketoglutarate and iron (By similarity). Demethylation of m6A mRNA affects mRNA processing and export (By similarity). Required for the late meiotic and haploid phases of spermatogenesis by mediating m6A demethylation in spermatocytes and round spermatids: m6A demethylation of target transcripts is required for correct splicing and the production of longer 3' UTR mRNAs in male germ cells (PubMed:23177736, PubMed:29279410).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG206184 | Alkbh5 (tGFP-tagged) - Mouse alkB, alkylation repair homolog 5 (E. coli) (Alkbh5) |
CNY 3710.00 |
|
| MR206184 | Alkbh5 (Myc-DDK-tagged) - Mouse alkB, alkylation repair homolog 5 (E. coli) (Alkbh5) |
CNY 3656.00 |
|
| MR206184L3 | Lenti ORF clone of Alkbh5 (Myc-DDK-tagged) - Mouse alkB, alkylation repair homolog 5 (E. coli) (Alkbh5) |
CNY 5230.00 |
|
| MR206184L4 | Lenti ORF clone of Alkbh5 (mGFP-tagged) - Mouse alkB, alkylation repair homolog 5 (E. coli) (Alkbh5) |
CNY 5230.00 |
