Pofut2 (NM_030262) Mouse Untagged Clone
CAT＃: MC211921
Pofut2 (untagged) - Mouse protein O-fucosyltransferase 2 (Pofut2), (10ug)
CNY 3990.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 2310011G23Rik; AI256847; BC003494; C21orf80; FUT13 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC211921 representing NM_030262
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGCTCAGCGTCGTCTGCCTGCTGCTGGCAGCGGCATCCTGGCGGCCGGTCTCGGCTTCAGGAG AAGAATTCTGGCCTGGCCAGTCGGCGGCGGACATCCTGTCGGGGGCGGCATCCCGCAGACGGTACCTCCT GTATGATGTCAACCCCCCAGAAGGCTTCAATCTCCGCAGGGATGTCTATATCCGGGTGGCATCCTTGCTG AAGACGCTGTTGAAGACTGAAGAGTGGGTGCTAGTCCTGCCCCCGTGGGGCCGCCTCTACCACTGGCAAA GCCCTGACATCCACCAGGTCCGGATTCCCTGGTCCGAGTTTTTTGACCTCCCGAGTCTCAATAAAAACAT CCCCGTTATAGAGTATGAGCAGTTCATTGCAGAGTCCGGCGGGCCCTTTATCGACCAGGTGTACGTCCTA CAAGGTTACGCCGAGGGCTGGAAGGAGGGCACCTGGGAGGAGAAGGTGGACGCACGGCCCTGTATTGACC CTCTGCTGTACTCGCAGGACAAGCATGAGTATTACAGAGGCTGGTTTTGGGGCTACGAAGAAACACGGGG TCTGAATGTCTCCTGCCTGTCCGTCCAGGGGTCGGCTTCCATCGTCGCGCCTGTCCTCTTAAAAAACACA TCTGCTCGGTCTGTGATGCTGGACCGAGCTGAGAATCTGCTTCATGACCACTATGGAGGCCGGGAGTACT GGGATACCCGGCGCAGCATGGTGTTTGCAAAGCACTTGCGGGCTGTGGGAGACGAGTTTAGGAGCCAGCA CCTCAACTCGACGGATGCCGCTGATAAGATGGCCCCGGAGGAAGACTGGACGAAGATGAAGGTCAAACTG GGCTCAGCACTAGGCGGCCCCTACCTCGGAGTCCACCTGAGAAGGAAGGACTTCATCTGGGGCCACAGGG AGGATGTGCCCAGCCTGGAGGGCGCCGTGAAGAAGATCCGCAGCCTCATGAAGACTCATCAGCTGGACAA GGTGTTCGTGGCCACAGACGCCATCAGGAAGGAGCAGGAAGAATTGAGGAAGCTGCTGCCGGAAATGGTG AGGTTTGAGCCCACGTGGGAGGAGCTGGAGCTGTACAAGGACGGAGGCGTCGCCATCATCGATCAGTGGA TCTGCGCTCATGCCAGGTTTTTTATTGGCACCTCTGTTTCCACATTTTCCTTTCGGATTCATGAAGAGAG AGAGATCCTGGGGTTGGACCCCAAGACAACATACAACCGGTTTTGTGGAGACCAGGAGAAAGCATGTGAG CAGCCCACACACTGGAAGATTGCGTACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_030262 |
| Insert Size | 1290 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_030262.3, NP_084538.2 |
| RefSeq Size | 2219 bp |
| RefSeq ORF | 1290 bp |
| Locus ID | 80294 |
| UniProt ID | Q8VHI3 |
| Gene Summary | Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in the consensus sequence C1-X(2,3)-S/T-C2-X(2)-G of thrombospondin type I repeats (TSRs) where C1 and C2 are the first and second cysteines of the repeat, respectively. O-fucosylates members of several protein families including the ADAMTS superfamily and the thrombosporin (TSP) and spondin families. Required for the proper secretion of ADAMTS family members such as ADAMSL1 and ADAMST13 (By similarity). O-fucosylation of TSRs is also required for restricting epithelial to mesenchymal transition (EMT), maintaining the correct patterning of mesoderm and localization of the definite endoderm.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG218637 | Pofut2 (tGFP-tagged) - Mouse protein O-fucosyltransferase 2 (Pofut2), (10ug) |
CNY 3140.00 |
|
| MR218637 | Pofut2 (Myc-DDK-tagged) - Mouse protein O-fucosyltransferase 2 (Pofut2) |
CNY 3656.00 |
|
| MR218637L3 | Lenti ORF clone of Pofut2 (Myc-DDK-tagged) - Mouse protein O-fucosyltransferase 2 (Pofut2) |
CNY 4750.00 |
|
| MR218637L4 | Lenti ORF clone of Pofut2 (mGFP-tagged) - Mouse protein O-fucosyltransferase 2 (Pofut2) |
CNY 4750.00 |
