Vhl (NM_009507) Mouse Untagged Clone
CAT＃: MC209640
Vhl (untagged) - Mouse von Hippel-Lindau tumor suppressor (Vhl), (10ug)
CNY 2400.00
CNY 3990.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | Vhlh |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC209640 representing NM_009507
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCCGGAAGGCAGCCAGTCCAGAGGAGGCGGCGGGGGAGCCCGGTCCTGAGGAGATGGAGGCTGGGC GGCCGCGGCCGGTGCTGCGCTCGGTGAACTCGCGCGAGCCCTCTCAGGTCATCTTCTGCAACCGCAGCCC GCGCGTCGTGCTGCCTTTGTGGCTCAACTTCGACGGCGAGCCTCAGCCCTACCCGATCTTACCACCGGGC ACCGGCCGCCGCATCCACAGCTACCGAGGTCATCTTTGGCTCTTCAGGGATGCGGGGACCCATGATGGAC TTCTGGTTAACCAAACGGAGCTGTTTGTGCCATCCCTCAATGTCGATGGACAGCCTATTTTTGCCAACAT CACATTGCCAGTGTATACCCTGAAAGAGCGGTGCCTTCAGGTTGTGCGGAGCCTGGTCAAGCCTGAGAAC TACAGGAGACTGGACATCGTCAGGTCACTCTATGAGGATTTGGAGGACTACCCAAGTGTGCGGAAGGACA TACAGCGACTGAGCCAAGAGCACCTTGAGAGTCAGCACCTGGAAGAGGAGCCTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_009507 |
| Insert Size | 546 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC052417, AAH52417 |
| RefSeq Size | 2730 bp |
| RefSeq ORF | 546 bp |
| Locus ID | 22346 |
| UniProt ID | P40338 |
| Gene Summary | Involved in the ubiquitination and subsequent proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Seems to act as a target recruitment subunit in the E3 ubiquitin ligase complex and recruits hydroxylated hypoxia-inducible factor (HIF) under normoxic conditions. Involved in transcriptional repression through interaction with HIF1A, HIF1AN and histone deacetylases. Ubiquitinates, in an oxygen-responsive manner, ADRB2 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG201630 | Vhl (tGFP-tagged) - Mouse von Hippel-Lindau syndrome homolog (Vhlh) |
CNY 2850.00 |
|
| MR201630 | Vhl (Myc-DDK-tagged) - Mouse von Hippel-Lindau tumor suppressor (Vhl) |
CNY 2400.00 |
|
| MR201630L3 | Lenti ORF clone of Vhl (Myc-DDK-tagged) - Mouse von Hippel-Lindau tumor suppressor (Vhl) |
CNY 4750.00 |
|
| MR201630L4 | Lenti ORF clone of Vhl (mGFP-tagged) - Mouse von Hippel-Lindau tumor suppressor (Vhl) |
CNY 4750.00 |
