Mesp2 (NM_008589) Mouse Untagged Clone
CAT＃: MC208960
Mesp2 (untagged) - Mouse mesoderm posterior 2 (Mesp2), (10ug)
CNY 3990.00
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Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | bHLHc6 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC208960 representing NM_008589
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCAGTCGCCTCCTCCTCAGAGCCTCCAGGGTCTCGACCACTGGGTCTTCTCCCAGGGCTGGGGCT GGGCTCAGCAATCGGACTCCACGTCTCCGGCCTCGTCCTCAGATTCGTCCGGTTCCTGCCCTTGCTACGC CACCCGTCGGCCCTCGCAGCCCGCCGGCCCGGCCCGTAGCACGCGCACTACCCAGGCGACGGCGCCCCGA CGAACGCGCCCAGCGCCCGCAGGCGGACAGCGGCAGAGCGCCAGCGAGCGCGAGAAGCTGCGCATGCGCA CACTCGCCCGCGCGCTGCAAGAACTGCGCCGCTTCCTGCCGCCGTCGGTGGCACCTGCAGGCCAGAGCCT GACCAAGATCGAGACGCTGCGCCTGGCCATCCGCTACATCGGCCACCTGTCAGCCCTGCTGGGCCTCAGC GAGGACAGTCTGCGGCGCAGGCGCCGACGGAGTGCGGACGCGGCGTTCTCTCACCGATGCCCTCAATGCC CCGACGGTGGCAGCCCCTCACAGGCTCAGATGCTTGGTCCTAGCCTGGGATCAGCCATGAGTAGTGGGGT GTCCTGGGGGTGCCCGCCTGCTTGTCCTGGACCTCTGATCTCACCTGAAAACCTTGGGAACAGGATCTCC AACGTGGATCCCTGGGTGACACCTCCTTATTGTCCCCAAATACAGTCACCCTTACACCAGTCCCTAGAAA GAGCCGCTGACTCCTCTCCCTGGGCACCACCTCAAGCATGTCCTGGCATGCAGATGTCCCCAGAGCCTAG GAACAAGACTGGACACTGGACACAATCCACTGAACCTGCAGAGCTGACTAAAGTGTATCAGAGTCTTTCT GTGTCTCCAGAACCCTGCCTGTCCCTGGGAAGCCCACTTCTCCTGCCCCGCCCATCATGCCAGAGACTAC AGCCTCAGCCTCAGCCTCAGCCTCAGTGGGGCTGCTGGGGCCACGATGCAGAGGTGCTCTCCACCTCTGA GGATCAGGGTTCCAGCCCTGCCCTCCAGCTTCCTGTGGCCAGCCCCACCCCCAGCTCAGGCCTGCAGCTC AGTGGCTGTCCTGAACTTTGGCAGGAAGACCTGGAAGGACCCCCACTGAATATTTTCTACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_008589 |
| Insert Size | 1113 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_008589.2, NP_032615.2 |
| RefSeq Size | 1936 bp |
| RefSeq ORF | 1113 bp |
| Locus ID | 17293 |
| UniProt ID | O08574 |
| Gene Summary | Transcription factor with important role in somitogenesis. Defines the rostrocaudal patterning of the somite by participating in distinct Notch pathways. Regulates also the FGF signaling pathway. Specifies the rostral half of the somites. Generates rostro-caudal polarity of somites by down-regulating in the presumptive rostral domain DLL1, a Notch ligand. Participates in the segment border formation by activating in the anterior presomitic mesoderm LFNG, a negative regulator of DLL1-Notch signaling. Acts as a strong suppressor of Notch activity. Together with MESP1 is involved in the epithelialization of somitic mesoderm and in the development of cardiac mesoderm. May play a role with Tcf15 in the differentiation of myotomal and sclerotomal cells by regulating Pax family genes. Controls also the expression of the protocadherin PCDH8/PAPC, EPHA4, RIPPLY2, NOTCH2, FGFR1, and CER1. Binds to the E-boxes within the EPH4A and RIPPLY2 enhancers.[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG222493 | Mesp2 (tGFP-tagged) - Mouse mesoderm posterior 2 (Mesp2), (10ug) |
CNY 3140.00 |
|
| MR222493 | Mesp2 (Myc-DDK-tagged) - Mouse mesoderm posterior 2 (Mesp2) |
CNY 3656.00 |
|
| MR222493L3 | Lenti ORF clone of Mesp2 (Myc-DDK-tagged) - Mouse mesoderm posterior 2 (Mesp2) |
CNY 4750.00 |
|
| MR222493L4 | Lenti ORF clone of Mesp2 (mGFP-tagged) - Mouse mesoderm posterior 2 (Mesp2) |
CNY 4750.00 |
