Eif2s2 (NM_026030) Mouse Untagged Clone
CAT＃: MC208067
Eif2s2 (untagged) - Mouse eukaryotic translation initiation factor 2, subunit 2 (beta) (Eif2s2), (10ug)
CNY 3990.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 38kDa; 2810026E11Rik; AA408636; AA571381; AA986487; AW822225; D2Ertd303e; EIF2; EIF2B |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC208067 representing NM_026030
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCGGGGACGAGATGATTTTTGATCCTACTATGAGCAAAAAGAAAAAGAAGAAGAAGAAGCCTTTTA TGTTAGATGAAGAAGGTGATGCCCAGACAGAAGAAACCCAGCCCTCAGAGACAAAAGAAGTGGAGCCAGA ACCAACTGAAGAAAAAGACGTGGACGCTGATGAGGAAGACAGTAGGAAGAAAGATGCTTCTGATGACTTA GATGATTTGAACTTCTTTAATCAAAAGAAAAAGAAGAAAAAGACAAAAAAGATATTTGACATTGATGAAG CTGAAGAAGCTATAAAGGATGTTAAGATTGAGAGTGATGCTCAAGAGCCAGCCGAGCCAGAGGATGACCT TGACATTATGCTTGGCAACAAAAAAAAGAAAAAGAAGAATGTTAAATTCCCAGAAGAAGATGAAATACTA GAAAAAGACGAAGCTTTAGAAGATGAAGACAGCAAAAAAGATGATGGCATTTCATTCAGTAGCCAAACTG CTTGGGCAGGCTCAGAAAGAGACTACACATATGAGGAGTTGCTGAACCGAGTGTTCAACATCATGAGAGA AAAGAATCCAGATATGGTTGCTGGAGAGAAGAGGAAATTTGTTATGAAACCTCCACAGGTCGTCCGAGTA GGAACCAAGAAAACTTCTTTTGTCAATTTTACAGATATCTGTAAACTATTACATCGTCAACCCAAACATC TCCTTGCATTTTTATTGGCAGAATTGGGTACAAGTGGTTCTATAGATGGTAATAACCAACTTGTAATCAA AGGAAGATTCCAACAGAAACAAATAGAAAATGTCTTGAGAAGATATATCAAGGAATATGTCACTTGTCAC ACATGCCGGTCACCGGACACAATCCTACAGAAGGACACCCGACTCTATTTCTTACAATGTGAAACTTGTC ATTCTCGATGCTCTGTTGCCAGTATCAAAACTGGCTTCCAGGCTGTCACAGGCAAGCGAGCACAGCTCCG TGCCAAAGCTAACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_026030 |
| Insert Size | 996 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_026030.2, NP_080306.1 |
| RefSeq Size | 2513 bp |
| RefSeq ORF | 996 bp |
| Locus ID | 67204 |
| UniProt ID | Q99L45 |
| Gene Summary | eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG204882 | Eif2s2 (tGFP-tagged) - Mouse eukaryotic translation initiation factor 2, subunit 2 (beta) (Eif2s2) |
CNY 2850.00 |
|
| MR204882 | Eif2s2 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 2, subunit 2 (beta) (Eif2s2) |
CNY 2400.00 |
|
| MR204882L3 | Lenti ORF clone of Eif2s2 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 2, subunit 2 (beta) (Eif2s2) |
CNY 4750.00 |
|
| MR204882L4 | Lenti ORF clone of Eif2s2 (mGFP-tagged) - Mouse eukaryotic translation initiation factor 2, subunit 2 (beta) (Eif2s2) |
CNY 4750.00 |
