Slc2a4 (BC014282) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Glut-4; Glut4
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC014282, the custom clone sequence may differ by one or more nucleotides ATGCCGTCGGGTTTCCAGCAGATCGGCTCTGACGATGGGGAACCCCCTCGGCAGCGAGTGACTGGAACAC TGGTCCTAGCTGTATTCTCAGCTGTGCTTGGCTCCCTTCAGTTTGGCTATAACATTGGGGTTATCAATGC CCCACAGAAGGTGATTGAACAGAGCTACAATGCAACGTGGCTGGGTAGGCAAGGTCCTGGGGGACCGGAT TCCATCCCACAAGGCACCCTCACTACGCTCTGGGCTCTCTCCGTGGCCATCTTCTCTGTGGGTGGCATGA TCTCTTCCTTTCTCATTGGCATCATTTCTCAATGGTTGGGAAGGAAAAGGGCTATGCTGGCCAACAATGT CTTGGCCGTGTTGGGGGGCGCCCTCATGGGCCTAGCCAATGCCGTGGCCTCCTATGAGATACTCATTCTT GGACGGTTCCTCATTGGCGCCTACTCAGGGCTAACATCAGGGCTGGTGCCCATGTATGTGGGAGAAATCG CCCCCACTCATCTTCGGGGTGCCTTGGGAACACTCAACCAACTGGCCATCGTCATTGGCATTCTGGTTGC CCAGGTGCTGGGCTTGGAGTCTATGCTGGGCACAGCTACCCTGTGGCCACTGCTTCTGGCTCTCACAGTA CTCCCTGCTCTCCTGCAGCTGATTCTGCTGCCCTTCTGTCCTGAGAGCCCCAGATACCTCTACATCATCC GGAACCTGGAGGGGCCTGCCCGAAAGAGTCTAAAGCGCCTGACCGGCTGGGCTGATGTGTCTGACGCACT AGCTGAGCTGAAGGATGAGAAACGGAAGTTGGAGAGAGAGCGTCCAATGTCCTTGCTCCAGCTCCTGGGC AGCCGCACCCACCGGCAGCCTCTTATCATCGCAGTGGTGCTGCAGCTGAGCCAACAGCTCTCAGGCATCA ATGCTGTTTTCTACTATTCAACCAGCATCTTCGAGTCGGCTGGGGTGGGACAGCCAGCCTACGCCACCAT AGGAGCTGGTGTGGTCAATACGGTCTTCACGTTGGTCTCGGTGCTCTTAGTAGAACGAGCTGGACGACGG ACACTCCATCTGTTGGGCCTGGCCGGCATGTGTGGCTGTGCCATCTTGATGACCGTGGCTCTGCTGCTGC TGGAACGGGTTCCAGCCATGAGCTATGTCTCCATCGTGGCCATATTTGGCTTTGTGGCCTTCTTTGAGAT TGGCCCTGGCCCCATTCCCTGGTTCATTGTGGCAGAGCTCTTCAGCCAGGGCCCCCGCCCAGCCGCCATG GCTGTCGCTGGTTTCTCCAACTGGACCTGTAACTTCATTGTCGGCATGGGTTTCCAGTATGTTGCGGATG CTATGGGTCCTTACGTCTTCCTTCTATTTGCCGTCCTCCTGCTTGGCTTCTTCATCTTCACCTTCCTAAA AGTGCCTGAAACCAGAGGCCGGACGTTTGACCAGATCTCAGCTGCCTTCCGACGGACACCTTCCCTTTTA GAGCAGGAGGTGAAACCCAGTACAGAACTTGAATACTTAGGGCCAGATGAGAATGACTGA
Restriction Sites	RsrII-NotI
ACCN	BC014282
Insert Size	1530 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC014282, AAH14282
RefSeq Size	2556 bp
RefSeq ORF	1530 bp
Locus ID	20528
Gene Summary	Insulin-regulated facilitative glucose transporter, which plays a key role in removal of glucose from circulation (PubMed:26240143, PubMed:26629404). Response to insulin is regulated by its intracellular localization: in the absence of insulin, it is efficiently retained intracellularly within storage compartments in muscle and fat cells (PubMed:26240143, PubMed:26629404). Upon insulin stimulation, translocates from these compartments to the cell surface where it transports glucose from the extracellular milieu into the cell (PubMed:26240143, PubMed:26629404).[UniProtKB/Swiss-Prot Function]

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