Keg1 (BC010803) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	0610008P16Rik; AI316519; GS4059
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC010803 CTTTGTTCTGAGGAAGCTCTCTGTGTGGATACGGAGTTATAGGTATCAGAAGAGATGTTCTGCTTACAGA GTTCCCAAGCACTGCAGGTTCTGGAGAATTCCCTAAGGAAGCACCTCCCTGAGTCCTTAAAGGTTTATGG GACTGTCTTCCACATCAATCAGGGAAACCCATTCAAGCTCAAGACTTTGGTGGACAAATGGCCTGATTTT AATACTGTTGTTATTCGACCCCAGGAAGAGGACATGACAGATGACCTTGACCACTACAACAACACTTATC TAGTGTATTCAAAGGATCCCAAGCACTGTCAGGAATTCCTTGGCTCATCAGAAGTTATTAACTGGAAACA ACATTTGCAGATTCAAAGTTCACAGTCAGACCTGGGCAAAGTGATAGAAAGTCTTGGAGCCACTAACTTG GGCAAGGTCAAGCATAAGCAGTGCTTTCTCTATATGGTATGCCAAACAGCAAAGAAACTGGCTCCTTCCT TGATGGATGCAAAGAACTTAGTAGTCAGCCGTAACAAGCTCACGCCTCTTGACCAGCAGTTATTCAAATT TGCCTCACTGGATGTTACACATGCTGCACTGGTGAATAGCCTCTGGCATTTTGGTGGCAATGAGAAGAGC CAGAAGTTCATTGAACGCTGTATCTTTACCTTCCCCAGCTTCTGTATTATGGGGCCTGAGGGGACCCCTG TGTCCTGGACCCTGATGGACCACACTGGAGAACTGAGAATGGGAGGCACCTTGCCTAAGTACCGGCGCCA GAGTCTCATTTACCATGTTGCTTCCCAGCAGATTCAGACACTAGAAAAGCTTGGCTTTCCCATGTATGCA CATGTGGATAAGGCTAACTTCACTGTTCAGAGAATGGTTGGCTTGCTGGGGCAAATCCTCTTGCCTTGTA CCTGGAACCAGTGGAACTGTGTACCGCTATAAAGCTAGAAAAGGGTGAGAAGGGGCCTGCGCTTGTAGAC GGAGAAATGGCTGAGAGAACAGAGAGAAGTGAACTGTCATTAGGATGGTGTGAGTTTCTAGAGAGCAGAG GTGCACCTCGCTGAGCTTCACACCCTGTTTCCGCGCGCTCACCTTGTTCATGCTCTTAACTCCATTCTTT GGCATGCAGAAGGCCACCTTCTCCTCCTCCACCCCTGTTACAGCTCCCACATTCTCAGGATTCCTCTAAC ACATTTGGCTAAATTGTACTCTTTTCTATTTGTTTTGCTGGCTCATTGATCCATGCCCATTAGTCAGTAG TTTGTGACCTATCCTGCACTCCCCTTGCACACAAGGCATACCTCCTCTGACTTTCTGTAGTTTTGCTTTT ATGGGCTGGAGAAGTAGCCCGTCATCTAAAAAGATGTTTTCAAAAATGATTCTTTCTCACGCCACTCACT AGGCAGTATATTGTTAATATGTAAAAACTACCATATATGCAAACACAGATGCACAATATGTGTGGTGCAA CTGTAAGAATTATTAGGAAAGAGACAAGTAAATGTGCTTGCAGCCAAGCCTGGAAACCTGAATGTGATTC CTGGGACCCATTTGTAAAAGGAGAGCATCAACTCCATTACCTTGCCCTCTGACTGCCACAGGTATGGAGC AGTATTTGCACACACAAATAAATAAATGTATTATTTCAAAAGTTAAAAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC010803
Insert Size	888 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC010803, AAH10803
RefSeq Size	1672 bp
RefSeq ORF	888 bp
Locus ID	64697
Gene Summary	Acyltransferase which transfers the acyl group to the N-terminus of glycine. Can conjugate a multitude of substrates to form a variety of N-acylglycines (By similarity).[UniProtKB/Swiss-Prot Function]

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