Aqp7 (BC022223) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AQP7L, AQPap, AQP 7
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC022223 GAGGCAGACTTGAATCTCCTTTCTGTTTGTTAGCTTGGTCTGCTGCTTCAGGTCCACCCACAACTCCACA ATGGCCCCCAGGTCTGTGCTGGAGACCATACAATCAGTGCTACAGAAGAATATGGTGCGAGAGTTTCTGG CAGAGTTCCTGAGTACCTATGTCATGATGGTGTTTGGCCTTGGTTCCGTGGCTCACATGGTTTTAGGAGA AAACTCTGGCAGCTATCTCGGTGTCAACTTGGGTTTTGGATTCGGAGTGACCATGGGAGTCCATGTAGCA GGCGGCATCTCTGGGGCCCACATGAATGCCGCAGTGACTTTCACCAATTGTGCACTAGGCCGAATGACCT GGAAGAAGTTCCCTGTATATGTGCTGGGTCAGTTCCTGGGCTCCTTCTCAGCTGCAGCTACCACCTACTT AATTTTCTATGGTGCCATTAACCACTTTGCAGGCGGAGACCTGTTGGTGACAGGTTCCAAGGCCACTGCA AACATTTTTGCCACCTATCTTCCTGAATACATGACACTGTGGCGGGGCTTCCTGGATGAGGCATTCGTGA CTGGGATGCTGCAGCTGTGTCTCTTCGCCATCACCGACAAGAAGAACAGTCCAGCACTTCAAGGGACCGA GCCCCTCGTGATAGGCATCCTTGTTACCGTCCTTGGGGTGTCGCTAGGCATGAACTCCGGATATGCAATC AACCCATCCCGTGACCTGCCTCCCCGGTTGTTCACTTTCATTGCTGGCTGGGGCAAACAAGTGTTCAGAG CCGGAAACAACTGGTGGTGGGTGCCGGTGGTGGCACCACTTCTGGGCGCCTACCTAGGTGGTATTGTATA CCTGGGTTTAATTCACCCCAGCATACCACAGGATCCTCAGAGACTGGAGAATTTTACAGCAAGAGACCAG AAGGTAACTGCATCATACAAGAATGCAGCTTCTGCAAACATAAGTGGCTCTGTGCCTCTAGAGCACTTCT AAGTAGAGCTTCTCTTTGACCACAACCGTACTGCAATAAAGCACACCACCTATCAGCGGTCCCCCACTCC TTGGGATCTCCTGCAGCTGAACTCCCTCCTAGGTTTTTCTGGGAGCTTCTGAGTTATTCCAGCCTGGTAC TTGTCGTGGAGAGCAGGCTGGCCTTGAACTTGTGATCTTCTTGCCTCTTTCTCCTGAGTGCTGGGGATAC AGGCATTTACTACCCTGCTTGGCATATTCCAGGGTGATTCTGGGCTCTTTTCTGAGGACTAGAGCATAGG GTCAGAAGACTCCAGCTCTTTCCAGTTCTAGGAGGTGGGGTGTCAAGGGGGAATGTCTACTTTGGGGGCT CAGGGTGAGTGATATAAAGGGCTTTCGTGCATCAGTATGTGTGTTGGGGGAACAGTGAGAAAAAGACCGG AGGTAGCTGATGGGAGATCAGATTTGGGGGTCTTTGACGGTTTTCTGGATAGGAGTACAGGATCTCCTGG CTTCTCCCTTCCTCTAGTTTTGAGCCAGAGAAGGTAAGGCTAAGAGTTTACATCAGAATTCCTCAACTTT TTTTTTTTTTTTTTTTGTTAATGACTTCCTCAGTGGCCTTGTAGTTCTCCACCCCTGTTTTTCTTAAAAT TATGTAGCCCAGGCTAGCCTTGACTGGAACTCTTATATAACTAAGTTTAGCCATGAACTCTGGATTGTCT GGCCTCCACCTCCTGAGTTCTGGGATTACAGGTGCGCACCACCATTCCCAGTTTATATGTGGTGCTGGGA TCAAACCCAAGGCCTTGTGTATGCTAGGTAATATCCATAACTAACTTAAATCCCAGCTCCCCAGTTTTTT CTTTTCTTGTCTTTTCTCTTTCTTTCTCTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTC TTTCTTTCTTTCTTCTTTCTTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTTCTTTTTT TTTTTCTTTTTTTGGTTTTTCAAGACAGGGTTTCTCGGTGTAGCCCTGGCTGTCCTGGAACTCACTTTGT AGACTAGGCTGGCCTCGAACTCAGAAATCCGCCTGCCTCTGCCTCCCAAGTGCTGGGATTAAAGGCGTGC GCCACCACTGCCCGGCCCAAATTTTTTCTTAATTAAAAATTTTAATACCACAAAAAAAAAAAAAAAAAAA AAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC022223
Insert Size	912 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC022223, AAH22223
RefSeq Size	2176 bp
RefSeq ORF	912 bp
Locus ID	11832
Gene Summary	Forms a channel that mediates water and glycerol transport across cell membranes at neutral pH (PubMed:15591341, PubMed:15746100, PubMed:16009937). The channel is also permeable to urea (By similarity). Plays an important role in body energy homeostasis under conditions that promote lipid catabolism, giving rise to glycerol and free fatty acids (PubMed:15591341, PubMed:16009937). Mediates glycerol export from adipocytes (PubMed:15591341, PubMed:15746100, PubMed:16009937). After release into the blood stream, glycerol is used for gluconeogenesis in the liver to maintain normal blood glucose levels and prevent fasting hypoglycemia (PubMed:15591341). Required for normal glycerol reabsorption in the kidney (PubMed:15998844, PubMed:17077387).[UniProtKB/Swiss-Prot Function]

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