Casq1 (BC064057) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	CSQ, CSQ1, CSQ-1
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC064057 CCTCTGTCCACTGCTCTGGCCCACTCTCTACCCTACCCCCACCTGGAGCCCCTAACTCAGGATTCTGTAT CCAAGGGGCACCTCTCCCTATTTCAGCTAACCTCCTCTGGACCAGGAGAGCAGGCCCAGATTGTACTACC TCCATGAGAGCTACCGACAGGATGGGGGCCAGAGCAGTGTCCGAGCTGCGGCTGGCACTGCTGTTTGTAC TGGTGCTAGGGACGCCCAGGTTAGGGGTCCAGGGGGAAGATGGGTTGGACTTCCCTGAGTACGACGGTGT GGACCGTGTGATCAATGTGAACGCCAAGAACTACAAGAACGTGTTTAAGAAGTATGAGGTGCTGGCCCTC CTCTACCATGAGCCCCCCGAGGACGACAAGGCCTCGCAGAGACAATTTGAGATGGAGGAGCTAATCCTGG AGTTAGCAGCCCAAGTCTTAGAAGACAAGGGTGTTGGCTTTGGCCTGGTGGACTCAGAGAAGGATGCAGC TGTGGCCAAGAAACTAGGACTAACTGAAGAAGACAGCGTTTATGTGTTCAAAGGAGATGAAGTCATTGAA TATGACGGCGAGTTTTCTGCAGACACTCTGGTGGAGTTTCTGCTTGATGTCCTAGAAGACCCTGTAGAGT TGATTGAAGGTGAACGAGAGCTGCAGGCATTTGAGAATATTGAAGATGAAATCAAACTCATTGGCTACTT CAAGAGCAAAGACTCAGAGCATTACAAAGCCTACGAGGACGCAGCTGAAGAGTTCCATCCCTACATCCCT TTCTTCGCTACCTTCGACAGCAAGGTGGCAAAGAAGCTGACTCTGAAGTTGAATGAGATTGATTTCTACG AGGCCTTCATGGAAGAGCCTATGACCATCCCAGACAAGCCCAACAGTGAAGAGGAGATTGTGAGCTTCGT GGAGGAGCACAGGATATCAACCCTGAGGAAACTGAAGCCTGAGAGTATGTACGAGACTTGGGAGGATGAC CTGGATGGAATCCACATTGTCGCCTTTGCAGAGGAAGCAGATCCTGATGGCTATGAGTTCTTAGAGACTC TCAAGGCTGTGGCCCAAGACAACACTGAGAACCCCGACCTCAGTATCATCTGGATTGATCCTGATGACTT CCCGCTGCTGGTCCCGTACTGGGAGAAGACCTTTGACATTGACCTGTCAGCTCCACAAATAGGAGTTGTC AATGTTACAGACGCGGACAGCATATGGATGGAGATGGATAACGAGGAGGACCTGCCTTCTGCTGATGAGC TGGAGGACTGGCTGGAGGACGTGCTGGAGGGCGAGATCAACACAGAGGATGACGACGACGATGACGACGA TGACGATGATGACGATGATGATGACGACTAGATGACTAGTGGCCGTGGCAGCCGCGTACCAGCCCCACTT GCTCCTTACTTGTTGTACCTTTCTGCCATCTGTGCCCTTCCCCGGGCTCCTTGGGACACTAGGTCATTCT CTACTACAGGGCCAACTGTGGTCTGCATACAGGGTGCTTGAGGTCCTGGTCTCCCTCTCCGAGGAGAAAA GGAGCTGCTTTTTCTTAGATCCCAGCCCAGTTCTCTCATCTGACTTGCTTCTTCCCCCAGACCTTATCTG TTTCTCTTCCCGCCACTCCCCATACTTAACGGTTTGCAAACTCTTCTCTATCCCATTTCTCAAATCTTAA ACCTCTCTCACTGTCCTGACCCTGGCCAGGAGGAAGGGAGGTACTGTGTTAGGGGCTATAATCTTAGCCA TCTCCTGTTAATGTATTTGGGTCAAATGCAAGGCCTTAATAAAGAAATCTGGGGCAGAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites	EcoRI-NotI
ACCN	BC064057
Insert Size	1200 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC064057, AAH64057
RefSeq Size	1877 bp
RefSeq ORF	1200 bp
Locus ID	12372
Gene Summary	Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle. Calcium ions are bound by clusters of acidic residues at the protein surface, often at the interface between subunits. Can bind around 80 Ca(2+) ions (By similarity). Regulates the release of lumenal Ca(2+) via the calcium release channel RYR1; this plays an important role in triggering muscle contraction. Negatively regulates store-operated Ca(2+) entry (SOCE) activity (By similarity).[UniProtKB/Swiss-Prot Function]

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