Wdr24 (BC037651) Mouse Untagged Clone
CAT＃: MC206644
Wdr24 (untagged) - Mouse WD repeat domain 24 (cDNA clone MGC:47001 IMAGE:5148210), (10ug)
CNY 5792.00
货期*
现货
规格
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Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | MGC47001 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC037651
CCACGCGTCCGGTGCGACGGGGCAGAGGCTTTTCTAGCACCTGGTTCTGGTCCCTCAGTTGCCGCTGCAC GGTGCAATTAGACACCTGCACTTATTGCTGTAGCCTCTCTTCTGCATTCCAAGGTGTATGAACTCAGTCC ATACCCATCTAGCTTCTCTGCCCTTTCCACTCGGTTGTTTTTCCTCTTGGAAGCCCCGCAATCCTCGGAG GCATGGGGCTGTGAAGTCAGGAGCCACTCCCCGCCCCCAGGATCTCACTGATGGAGAATTAACCACATAG AATCGGGAGACGTCAGATATGGTGCTGACGAACCTCAGGTTAGCTCTGGAGCCTTCTGGAGAACTCCAAT GAATGTGAACCTTCCTAAGATAACGACTGGCTGATGGACCATCTCAGGCCGAAGGTTCGAGAGGAGACTG AACCTGAATTGTTAGGACACCATGGCTGACCAGGTCAGCACACTTCACTGACCTCCTCCTGCCTCTGATA CCTGTGCAGACATGGAAAAGATGTCCCGAGTTAGCACAGCCCTGAGCGGCAGTGCTCTGACGGGCCGAAC CATGCACTGCCATCTGGACGCGCCAGCTAATGCCATCAGCGTGTGCCGCGACGCTGCCCAGGTTGTCGTG GCAGGCCGAAGCATCTTCAAGATTTATGCCATTGAGGAGGAGCAGTTCGTAGAGAAGCTGAATTTACGTG TGGGTAGGAAGCCCTCGCTCAACCTGAGCTGTGCTGATGTGGTCTGGCACCAGATGGATGAGAATCTGCT GGCCACAGCAGCCACCAATGGTGTGGTGGTTACATGGAACCTAGGTCGGCCATCCCGCAACAAGCAGGAT CAGCTGTTCACAGAACACAAGCGCACAGTGAACAAAGTCTGTTTCCATCCCACCGAGGCCCATGTGCTGC TCAGTGGCTCTCAGGATGGCTTCATGAAGTGCTTTGACCTCCGAAGGAAGGACTCTGTCAGCACCTTCTC TGGCCAGTCTGAGAGCGTGCGGGATGTGCAGTTCAGTATCCGTGACTACTTTACCTTTGCTTCCACCTTT GAGAATGGCAACGTACAGCTCTGGGACATCCGCCGACCCGACCGCTGTGAGAGGATGTTCACAGCCCACA ATGGGCCTGTCTTCTGCTGTGATTGGCACCCAGAGGACAGGGGCTGGCTGGCCACGGGTGGACGTGACAA GATGGTAAAGGTCTGGGACATGACCACACATCGTGCCAAAGAGATACACTGTGTGCAGACAATCGCTTCA GTGGCCCGAGTCAAGTGGCGGCCTGAATGCCGCCACCACCTGGCCACGTGTTCCATGATGGTGGACCACA ACATCTATGTATGGGATGTACGCCGCCCCTTCGTGCCAGCTGCTATGTTTGAAGAGCATCGTGATGTCAC AACAGGCATTGCCTGGCGTCATCCGCATGACCCCTCTTTCCTGCTCTCCGGCTCCAAGGACAGCACGTTA TGCCAGCACCTGTTTCGGGATGCCAGTCAGCCTGTTGAGCGTGCCAACCCTGAGGGTCTCTGCTATGGCC TCTTTGGGGACCTGGCCTTTGCTGTTAAGGAAAGCCTGGTGGCTGCTGAATCTGGACGTAAACCCTATGC TGGTGACCGGCGCCACCCCATCTTCTTCAAGCGCAAGTTGGACCCTGCAGAACCTTTTTCTGGCCTCGCT TCCAGTGCACTGAGCGTCTTTGAGACGGAGTCTAGCGGTGGCAGCATGAGCTGGTTCGTGGATACAGCTG AACGTTATGTTCTGGCTGGCCGGCCACTAGCCGAGCTCTGTGATCACAATGCAAAAGTGGCTCGGGAGCT TGGCCGCAATCAGGTGGCACAGACATGGACCATGTTACGGATTATCTACTGTAGCCCTGGCTTGGTATCC TCTGCCAATCTCAACCACAGCGTCGGCAAAGGCAGCTCCTGCGGCCTACCGCTCATGAACAGCTTCAATC TGAAGGATATGGGCCCGGGGCTGGGCAGTGAGACACGGCTGGATCGCAGCAAAGGGGACACACGGAGTGA TGCGGCGCTGCTTGACTCTTCAGCCACACTCGTCACCAATGAGGATAATGAGGAAACAGAAGGGAGTGAT GTGCCTGCTGACTACCTGCTGGGTGACGTAGAAGGAGAGGACGATGAGCTGTACCCACTTGACACAGAAC ATGTGCACTCCGAGGAGCCTGAATATGTGCTGCCACAAGAGGCCTTCCCACTGCGCCATGAAATTGTAGA CACACCATCTGGGCCCGAGCATCTGCAGGACAAGGCGGACTCTCCGCACGTGAGCGGCAATGAGGCAGAC ACAGCTTCATTAGCCCCGGTGGACTCCTCCTCCTCCCTCCTCTCCGTCTCACATGCACTCTATGACAGCC GCCTGCCACCTGACTTCTTCAGTGTATTGGTACGGGACATGTTGCGCTTCTACGCTGAGCAGGGCGACGT GCAGATGGCTGTGTCTGTTCTCATCGTGCTGGGTGAGAGAGTGCGAAAGGACATCGATGAGCAGACCCAG GAGCACTGGTACACATCCTACATAGATCTGCTGCAGCGCTTCTGCCTCTGGAATGTGTCCAATGAGGTGG TGAAGCTGAGCACCAGCAGGGCAGTTAGCTGCCTCAACCAAGCCTCCACCACGCTGCATGTCAACTGTAG TCACTGCAAGCGGCCCATGAGCAGCCGCGGCTGGGTCTGTGACAGGTGCCACCGCTGTGCCAGCATGTGT GCTGTCTGCCATCATGTGGTTAAAGGCCTGTTCGTGTGGTGTCAGGGTTGCAGTCATGGTGGCCATCTTC AGCACATCATGAAATGGCTGGAAGGCAGCTCCCACTGCCCTGCAGGCTGCGGCCACCTCTGTGAATACTC CTGACCTGGCCTTCCCTAAGGCTGATGCAGCGTCCTCAGCTCGATCCCGATGTCCACCGGACTAGGCTCA CAGCCCCTTACCCATCCTGTGTACCAGGATGGAAGGTGGCTACAACTTTTTAACCCAATAAAAATCCAGA GCTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | EcoRI-NotI |
| ACCN | BC037651 |
| Insert Size | 2373 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC037651, AAH37651 |
| RefSeq Size | 3059 bp |
| RefSeq ORF | 2373 bp |
| Locus ID | 268933 |
| Gene Summary | As a component of the GATOR subcomplex GATOR2, functions within the amino acid-sensing branch of the TORC1 signaling pathway. Indirectly activates mTORC1 and the TORC1 signaling pathway through the inhibition of the GATOR1 subcomplex. It is negatively regulated by the upstream amino acid sensors SESN2 and CASTOR1. In addition to its role in regulation of the TORC1 complex, promotes the acidification of lysosomes and facilitates autophagic flux.[UniProtKB/Swiss-Prot Function] |
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