Hsf2 (BC018414) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI661205
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC018414 CTCCGCGTTCGGTAGTAGAATTTGGAATCCCTGCACCGGGCTAACAATGAAGCAGAGTTCCAACGTGCCG GCTTTCCTCAGCAAGCTGTGGACGCTTGTGGAGGAAACCCACACCAACGAGTTCATCACCTGGAGTCAGA ATGGACAAAGTTTTCTGGTCTTGGATGAGCAAAGATTTGCAAAGGAAATTCTTCCTAAGTACTTCAAACA CAATAACATGGCGAGCTTTGTGAGACAACTAAATATGTATGGCTTCCGAAAAGTAGTGCATATCGAATCT GGAATTATCAAACAGGAAAGAGATGGCCCTGTTGAATTTCAGCATCCTTATTTCAAGCAAGGCCAGGATG ACCTGTTGGAGAACATTAAAAGGAAGGTTTCATCTTCAAAACCAGAGGAAAATAAAATTCGTCAGGAAGA TTTAACAAAAATTATTAGTAGTGCTCAGAAGGTTCAAATAAAACAAGAAACTATTGAGTCCAGGCTTTCA GAATTAAAAAGTGAGAATGAATCCCTTTGGAAGGAGGTGTCAGAACTAAGAGCAAAGCATGCCCAGCAGC AACAAGTTATTCGGAAGATTGTCCAGTTTATTGTTACATTGGTTCAGAATAATCAACTTGTGAGTTTAAA ACGTAAAAGGCCTCTACTTCTAAACACAAATGGAGCCCCAAAGAAGAATCTATATCAGCACATAGTCAAA GAACCAACTGATAATCACCATCATAAAGTTCCACACAGCAGGACTGAAGGTTTAAAGTCAAGAGAACGGA TTTCAGATGACATAATTATTTATGATGTTACTGACGATAATGTGGATGAAGAAAATATTCCAGTTATTCC AGAAACAAATGAGGATGTTGTAGTGGATTCCTCCAACCAGTATCCTGACATTGTCATTGTTGAAGATGAC AACGAGGATGAGTATGCTCCTGTCATTCAGAGTGGAGAGCAGAGTGAACCAGCCAGAGAACCCTTACGTG TGGGGAGTGCTGGCAGCAGCAGCCCTCTCATGTCCAGTGCTGTCCAGCTAAACGGCTCCTCCAATCTGAC CTCAGACGACCCTGTGACCATGATGGACTCCATTCTGAATGACAACATTAACCTGTTAGGAAAGGTTGAG CTGTTGGATTACCTTGACAGTATTGATTGCAGTTTAGAGGACTTCCAAGCTATGCTCTCAGGAAGACAGT TTAGCATAGACCCAGATCTTCTGGTTGATTCTGAGAATAAGGGACTAGAAGCTACCAAGAGCAGTGTTGT TCAACACGTGTCAGAAGAGGGAAGAAAATCTAAATCCAAGCCAGACAAACAACTTATCCAGTATACTGCC TTTCCACTTCTTGCATTCCTGGATGGGAACTCTGCATCTGCTATTGAACAGGGGAGTACAACTGCATCGT CAGAAGTTGTGCCTTCTGTAGATAAGCCCATAGAGGTCGATGAGCTCCTGGATAGCAGCCTGGATCCAGA ACCGACCCAGAGTAAGCTTGTCCGCCTGGAACCATTGACTGAAGCGGAAGCTAGTGAAGCCACACTCTTC TATTTATGTGAACTTGCTCCTGCACCTCTGGATAGTGATATGCCGCTTTTAGATAGTTAAACTCCCAAGT GGACACTGTATATATTCATCAAAATGATGAAGTATTTATTTTAAAGTATCATTTGGTACTTTGTTTTGTA AATTACTTTTTGTTTAATCAGATACTGTGGAATCAAAGCACCTTTTGCTTTTCTCACTAACCATACTCTC ACATAGCTTTCAGATGCTACTCGACTGCCTAGGCATATACGTTTTAATGCACATTACTGGCATATCTTAA GTTCAATTCAAATGGCCATTTTCTCCAGTTGTAATTGGATTTTTTTTTTTTTACAAATATGACCATTAAT CCCAGCTAAACTTGTTTATTTTCAATTAAGTGTCTAGAACTAAAGTAGAAAACTGGCCCAGTTACTTTTA TTAAATATTTTTCACTTGGCTTATTTCTAAAACTGGGAACATAAAGTGCCTGTATCTTGTAATATTTCAT GTCTTTGTTGGTTCAGAGAAATTTATTCCTGTTAGCAGAAATGGCAAAGTGTACACGAGTACTCAGTCAC TGATGTGGTACTAGCTCCAGATACATAGAGAATGTGGAGACCGGGTGTGCTTAGCTCCACCCATTTAGAC TAGTTTAAGTATGTTTTCTGGTTTTTGTTCTTTTGAACCATGATATTTAGTAAAATAAATATGATGGATG TCGAGGAAAAAAAAAAAAAAAAA
Restriction Sites	EcoRI-NotI
ACCN	BC018414
Insert Size	1554 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC018414, AAH18414
RefSeq Size	2263 bp
RefSeq ORF	1554 bp
Locus ID	15500
Gene Summary	DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. HSF2 is expressed in a form that binds DNA constitutively but loses DNA binding by incubation at greater than 41 degrees C.[UniProtKB/Swiss-Prot Function]

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