Itgb1bp1 (BC028772) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI449260; AU019480; Icap1
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC028772 CTTCTCTGGATCGAGCTATGGTGAAAGATGTTTCGCAAAGGCAAGAAGAGACACAGCAGCAGCAGTTCCC AGAGCAGTGAAATCAGTACAAAGAGCAAGTCTGTGGATTCCAGCCTTGGAGGACTTTCAAGATCCAGCAC TGTCGCCAGCCTCGATACAGACTCCACTAAAAGCTCAGGACAGAGTAACAGCAATTTAGACACATGTGCT GAGTTCCGAATAAAGTATGTTGGTGCCATCGAGAAACTGGCCGTGTCTGAGGGGAAGAGCCTTGAAGGAC CACTAGACCTGATAAATTACATAGATGTCGCCCAGCAAGATGGAAAGTTGCCTTTTGTGCCTTTGGAAGA GGAGTTCATTCTGGGCGTTTCTAAGTACGGCATAAAAGTCTCGACCACGGATCAGCATGGTGTTCTGCAT AGGCATGCCCTGTATCTAATCATCCGGATGGTGTGTTACGATGACGGCCTGGGAGCTGGGAAAAGCTTGC TGGCACTCAAGACCACAGATGCAAGCAACGAAGAGTACAGCCTGTGGGTTTACCAGTGTAACAGCCTGGA GCAAGCCCAAGCAATCTGCAAGGTCTTATCCACAGCATTTGACTCTGTGTTGACCTCTGACAAGTCCTGA ACTCTGCAGGCAAGTGGAAACTGACCCACGGAGCTTACCTGTTCCTACATCTATCTGCAGCTGTGGACTA TTAGAGGGTGTAATGCCCTGACCCGGTTTTCTAAAGTGTAATGTATGAAATATTGGTTTTATACTAAAAG GTGTTAACAGTAGGTCAACTTCCAATAAATTACCTGCTTTCTGGAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites	EcoRI-NotI
ACCN	BC028772
Insert Size	603 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC028772, AAH28772
RefSeq Size	837 bp
RefSeq ORF	603 bp
Locus ID	16413
Gene Summary	Key regulator of the integrin-mediated cell-matrix interaction signaling by binding to the ITGB1 cytoplasmic tail and preventing the activation of integrin alpha-5/beta-1 (heterodimer of ITGA5 and ITGB1) by talin or FERMT1. Plays a role in cell proliferation, differentiation, spreading, adhesion and migration in the context of mineralization and bone development and angiogenesis. Stimulates cellular proliferation in a fibronectin-dependent manner. Involved in the regulation of beta-1 integrin-containing focal adhesion (FA) site dynamics by controlling its assembly rate during cell adhesion; inhibits beta-1 integrin clustering within FA by directly competing with talin TLN1, and hence stimulates osteoblast spreading and migration in a fibronectin-and/or collagen-dependent manner. Acts as a guanine nucleotide dissociation inhibitor (GDI) by regulating Rho family GTPases during integrin-mediated cell matrix adhesion; reduces the level of active GTP-bound form of both CDC42 and RAC1 GTPases upon cell adhesion to fibronectin. Stimulates the release of active CDC42 from the membranes to maintain it in an inactive cytoplasmic pool. Participates in the translocation of the Rho-associated protein kinase ROCK1 to membrane ruffles at cell leading edges of the cell membrane, leading to an increase of myoblast cell migration on laminin. Plays a role in bone mineralization at a late stage of osteoblast differentiation; modulates the dynamic formation of focal adhesions into fibrillar adhesions, which are adhesive structures responsible for fibronectin deposition and fibrillogenesis. Plays a role in blood vessel development; acts as a negative regulator of angiogenesis by attenuating endothelial cell proliferation and migration, lumen formation and sprouting angiogenesis by promoting AKT phosphorylation and inhibiting ERK1/2 phosphorylation through activation of the Notch signaling pathway. Promotes transcriptional activity of the MYC promoter.[UniProtKB/Swiss-Prot Function]

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