Fbxw5 (BC010776) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI159739; Fbw5
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC010776 GAAGCCGGGCGCAGCGCAGCGGGCAGAGGCACCGCGGACCGTCTGGGCAGAATGTCACGATGGATGAGGG GGGCCTGCCCCTGCTCCCCGACAGCCTTGTCTACCAGATCTTCCTGAGTTTGGGCCCTGCAGATGTGCTG GCTGCTGGGCTGGTATGCCGCCAATGGCAGGCTGTGTCCCGGGATGAGTTCTTATGGAAGGAGCAATTCT ACCGCTACTACCAGGTGGCTCGAGATGTGCCCCGCCACCCAGCGGCCACATCCTGGTATGAGGAGTTCCG GCGGCTCTATGACATGGTACCCTGTGTGGAGGTGCAGACACTGAAGGAGCACACTGACCAGGTCCTGCAC CTCAGCTTCTCCCACTCGGGGTACCAGTTTGCCTCCTGCTCCAAGGACTGCACGGTGAAGATCTGGAACA ACGACCTGACCATCTCACTGCTGCACAGTGCAGATATGAGGCCATACAACTGGAGTTACACCCAGTTTTC CCAGTTCAACCAGGATGACTCACTGCTGCTGGCCTCAGGGGTGTTCCTGGGGCCACATAACTCCTCCTCA GGCGAGATAGCTGTCATCAGCTTAGACTCCTTCGCCCTGCTGTCCCGTGTGCGAAACAAGCCCTATGATG TGTTTGGCTGCTGGCTCACAGAAACCAGCCTCATTTCGGGGAACCTGCACCGCATTGGAGATATCACCTC CTGCTCAGTGCTGTGGCTCAACAATGCCTTCCAGGATGTGGAGTCAGAGAATGTCAACGTGGTAAAGCGG CTCTTTAAGATCCAGAACCTCAATGCCAGCACCATCCGCACAGTGATGGTGGCTGACTGTAGCCGCTTTG ATAGCCCGGACCTCCTGCTGGATGCTAGCGACCAGGCTGGGCTCCCCTGCCGAGTCTTTGACCTAGGTGG GGACACTGAAGAAGAGGCCACTGACCCAGGTTTGCACACTTCTGGTTCTGACCACGTCAAGAAAGGTTTA CGGCGTGTGTTTGATAGCGTCTTGGATGGACACGGACAGCTATCGGATTGTGCACTGGAGACCAAGGTGG CTGAGCTGCTGGCCCAGGGCCACACCAAGCCTCCAGAGTGCAATGACGCTGACACCAGGAATAAGTACCT CATCTTCACCACTGGCTGCCTCACGTACTCACCGCATCAGATCGGCATCAAGCAGATCCTGCCACACCAG ATGACAACTGCAGGGCCCGTGCTGGGCGAGGGCCGGGGCTCTGATGCCTTCTTTGATGCCCTGGACCATG TCATTGATGTGCATGGGCACATCATTGGCATGGGCCTGTCTCCTGATAACAGGTACCTGTATGTGAATAG CCGTGCCTGGCCCCCTGGCTCAGTGGTAGCCGACCCCATGCAGCCACCACCCATTGCGGAGGAGATTGAC TTGCTGGTGTTTGATCTCAAGACCATGCGAGAGGTGAAGCGAGCTCTTCGAGCGCACCGTGCCTACACAC CCAATGACGAGTGCTTCTTCATCTTCTTGGATGTCAGCAGGGATTTTGTGGCCAGTGGGGCTGAAGATCG GCATGGCTATATCTGGGACCGCCACTACAACATCTGCCTAGCCAAGCTGCGGCATGAGGATGTGGTCAAC TCCGTGGCTTTTAGCCCCCAGGAGCAGGAACTCTTGCTGACAGCCAGCGACGATGCCACTATCAAAGCCT GGCGTTCACCACGCATTGTTCGTGTTCTGCAGGCTCCACGCCCTCGCCCCCGCCCTCGCCCCCGCCCCTT CTTCTCCTGGTTTGCCAGCCATAGGCGCTGAAGGCTTCAGTGATTTGAGCCATTGGAACCTACTGGGGAA AATGGTACCCCGTGGTTCTTTATTGCACTGGGGCAGAGTAGCTCATAGCAGCAATGCCTTAGGAGGGGCA GCCTGACCCCAACACACACACACACACACACACACACAAAACCTGCTCACTTAGCCATAAGGCTTAGCAC TAGGGTAGCCCTTGTTGGCAGCACCAGATCCCATGCTAGCCTCTTGGCTAGCTTGGGGTACACAGGTCAT TGGGAACCCTGTTCTTCACCTACCCTCTTCCCTTGGGTTGGCAGCTCACACAGGTCCAAGCTGGCTTCTG CCTGGGTACCTTTCATATTCAGCCAAACCCACACCCTTGGATGTCTTGAGTGTACCCTGGGCAGCAGGCC ACAGGCACTGGTCTCTGATAATTCAGTTTTTGCTACTGAGTGGCCTGCAGTGGACTGTGGGTGCTGCATG CTGCACTCCTGAACTGTTTGGGCCTGAATAAACAGTTGGCAACAGCAAAAAAAAAAAAAAA
Restriction Sites	EcoRI-NotI
ACCN	BC010776
Insert Size	1722 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC010776, AAH10776
RefSeq Size	2301 bp
RefSeq ORF	1722 bp
Locus ID	30839
Gene Summary	Substrate recognition component of both SCF (SKP1-CUL1-F-box protein) and DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes. Substrate-specific adapter of the DCX(FBXW5) E3 ubiquitin-protein ligase complex which mediates the polyubiquitination and subsequent degradation of TSC2. May also act as a negative regulator of MAP3K7/TAK1 signaling in the interleukin-1B (IL1B) signaling pathway. Substrate recognition component of the SCF(FBXW5) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of SASS6 during S phase, leading to prevent centriole reduplication (By similarity). The SCF(FBXW5) complex also mediates ubiquitination and degradation of actin-regulator EPS8 during G2 phase, leading to the transient degradation of EPS8 and subsequent cell shape changes required to allow mitotic progression.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.