Rnf186 (BC011492) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	9130020G10Rik
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC011492, the custom clone sequence may differ by one or more nucleotides ATGTCCTGCACTGAGGCCCCACAGCCTATCCCAGCGGGTACCACCACCACCAGCACCATCATTGCCTTGG GGCCCACCGGGCGGCTCAGCATCTCTGTGGAGGGTGACCTGGAATGCTTGGTGTGCCGAGAGCCCTACAA CTGTGCTCGGTCCCCCAAGCTGCTTAGCTGTCAGCATACCTTCTGTGCCGTATGCCTGAAGCTTCTGCTA TATGTGCAGGAAGACACCTGGTCCATCCCCTGTCCGCTGTGCCGAAAGGTCACTGCTGTCCCGGGAGGCC TCATCTGCAGCTTGCGAGACCAGGAGGCGATGGTGGGGCGTCTGGCCCTGCCATGCCCAGAGGTGCGCCT ATGTCCTCAGAGGCTAGTGGGTTCTGCCGCTTCAGCAACTCGGCCAGCCAACTGGACAGGAGAAGAAGAA CAGGACACTGTAAGTGTCAACCGTGTAGCTGCCCGGCGCCTGGCTGTACACTTGCTCTTGTTGGCTCTTG TCATTGTCCTTATCCTGCCTTTCATCTACCCGGGTGTCATCCGGTGGGTGTTGGCCTTTGTCATTGCTCT GGCTCTCCTGATGTCCACCCTATTCTGCTGTCACCCCCAGAGCCAGAACAGCAACTGGCTTTGCCCCAGG ACTCTGTTCTGCAGAGAGCAAAAGCAAACCCAGATCACTTCTATTGCCTGA
Restriction Sites	EcoRI-NotI
ACCN	BC011492
Insert Size	681 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC011492, AAH11492
RefSeq Size	1237 bp
RefSeq ORF	681 bp
Locus ID	66825
Gene Summary	E3 ubiquitin protein ligase that is part of an apoptotic signaling pathway activated by endoplasmic reticulum stress. In that process, stimulates the expression of proteins specific of the unfolded protein response (UPR), ubiquitinates BNIP1 and regulates its localization to the mitochondrion and induces calcium release from the endoplasmic reticulum that ultimately leads to cell apoptosis.[UniProtKB/Swiss-Prot Function]

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