Sf3b5 (BC006603) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	10kDa, Sf3b10
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC006603 AGACTGTCTACTCCTGCGTCTCGGTGGCGTCTTTCTCCCGCGCCTGCACGAACTGAGGTTTTGCGTGGCG GCGGCGGCACCGGCAGCGGCAGCGTCTCTCACTTGAACGCCGCGAGCGGCAGCTTCTCGTCTGTGTCCTG ACCTCGGAGCCTACGAGCAGAGCGGCGCGATGACGGACCGGTACACCATCCACAGCCAGCTGGAGCATCT GCAGTCCAAGTACATCGGCACGGGCCACGCCGACACCACCAAGTGGGAATGGCTGGTGAACCAGCACCGC GACTCCTACTGCTCCTACATGGGCCACTTCGACCTCCTCAACTACTTCGCCATTGCCGAGAACGAGAGCA AAGCCCGCGTGCGGTTCAACCTGATGGAGAAAATGCTGCAGCCCAGCGGGCCGCCCGCGGACAAGCCCGA GGAGAACTGAGGCGAGCGCTGCCCAGTCTTCCCCGTGTGCCGGCTGCGAGCCTCCTTGCTCCTGCATCTC GACCATTCCGTGTTGGCTGTATCGCCTGACCTGCGTACCTGTGGAGGATTCGGAACAAGTCATGGAGAGA CTGTCCGGGTCCGCTCCTTGTGAACTGTGCAGAAGGAGTGATCCCAGCATCGGCAAGCGAGGGAGAAGAC TGCACGAGAGTGATGGCGCATTTCGAGTCTGCTTTTCGATAGTTGATGTCTTCTTGCCTTTTTAAAAAAC CACATATATATAAAAAAAAAAAAAAAA
Restriction Sites	EcoRI-NotI
ACCN	BC006603
Insert Size	261 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC006603, AAH06603
RefSeq Size	727 bp
RefSeq ORF	261 bp
Locus ID	66125
Gene Summary	Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex, a constituent of the spliceosome. SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.