Gnl2 (BC003262) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	MGC7863, Ngp-1, HUMAUANTIG
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC003262 CCAACGTGCTTCACACAGGACGCTTTCCCGGCTGGGAAGTCATGAAGTGGTTTACCTCGCGAGTAGCAGA GCGGTGTCGGTACTGTCGTCAGGATGGTGAAGCCCAAGTACAAAGGACGGAGCACCATCAACCGCTCGGC GGCCAGCACCAACCCAGATCGAGTACAGGGAGCTGGCGGCCAAAACATGAGGGATCGGGGCACAATTCGG CGCCTGAATATGTACAGGCAAAAGGAGCGCAGGAACAGTCGTGGTAAAGTGATTAAGCCACTGCAGTATC AGTCAACTGTGGCTTCTGGCACAGTGGCCCGAGTGGAGCCGAATATTAAATGGTTTGGAAATACTCGTGT GATCAAGCAGGCATCATTACAAAAATTTCAAGAGGAAATGGATAAAGTTATGAAGGATCCATACAAAGTT GTCATGAAACAAAGCAAATTACCGATGTCTCTTCTGCACGATCGAATCCAGCCTCATAACGCAAAAGTCC ACATTCTTGATACTGAAAGCTTTGAAAGTACATTTGGCCCAAAGTCACAGAGAAAGCGGCCAAACTTGTT TGCAAGTGATATGCAATCCCTTCTAGAAAACGCTGAAATGTCTACTGAGAGCTATGACCAGGGCAAGGAC CGTGATTTGGTGATGGAGGACACTGGTGTAAGAAATGAAGCTCAAGAAGAGATATATAAAAAAGGGCAGT CAAAAAGAATATGGGGAGAACTCTACAAGGTGATAGACTCATCAGATGTTGTCGTTCAAGTCCTTGACGC TAGAGATCCGATGGGCACTCGTTCCCCCCACATCGAAGCTTACTTGAAAAAGGAAAAACCCTGGAAACAT CTCATTTTTGTACTCAATAAGTGTGACCTTGTTCCAACTTGGGCAACTAAACGATGGGTTGCTGTGCTCT CCCAGGACTACCCAACACTGGCTTTCCATGCGAGCCTCACCAATCCCTTTGGGAAGGGAGCATTCATTCA GCTTCTGCGGCAGTTTGGGAAGTTGCACACAGACAAGAAACAAATCAGTGTTGGGTTCATTGGCTATCCA AATGTAGGCAAGAGCTCTGTGATTAATACATTACGATCCAAGAAAGTTTGCAACGTGGCCCCCATTGCTG GAGAAACAAAGGTCTGGCAGTATATTACCTTGATGCGTCGTATATTCCTGATTGACTGCCCTGGTGTGGT TTACCCATCTGAGGACTCAGAGACCGACATTGTGCTCAAAGGAGTGGTTCAAGTTGAGAAAATTAAAGCT CCTCAAGACCACATTGGTGCTGTCCTTGAACGAGCAAAGCCAGAGTATATCAGCAAGACGTACAAGATTG AGTCCTGGGAGAACGCGGAGGACTTTCTTGAGAAGCTAGCTCTCCGCACTGGGAAGTTACTGAAGGGTGG AGAGCCTGACATGCTGACTGTGAGCAAGATGGTTCTCAATGACTGGCAGAGAGGCCGAATCCCTTTCTTT GTCAAGCCGCCCAATGCAGAGATACCGACCGATTCCCAGCTTCCACCATCCTCACCATTGGAAGTTCCCA CAGAAACAACCCAGAACAACCCAGAAGAAGAGACCACAGAAACAGAAGTTGAAAGGTCAGACTCTATCAC TGAAAAGGAGCCAGAAGGAGACTGTTCTCAGGATAGAAACTCAGAGATGCAACAGATCCTCGCACGAGTT CGCCAGAACTTTGGCAAAATCAACGTGGGGCCTCAGTTTTCTGCGGATGACCTGGTGCCTGTGGAGATGT CAGACTTGGAAGATCTGGAAAGCTCTGGGGAAGAGGAAGAACAGGAGCAGGAACAGCCAGGGGAGGATGC CGAGGAAGAGCGCTCCCCAGACACTCAGGAGGAACCAGTGGGAAACGACACCAAGGCCGTGCTCAGAGCC CTGGATGAGAAGATTGCCAAGTACCAGAGGTTTCTAAATAAAGCCAAAGCTAAAAAGTTCTCTGCCGTCA GAATATCCAAGGACTTAAGTGAAAAGGTTTTTGCAAAATACAAAGAAGAGAAGAAAACATCTGCAGAAGT CAGTGATGCAGCACCCACCAAAAAGGCAAGGAAGTGGGATGCACAGATGGAAGAAGAACCTTCAAATAAG ACTCAGAGGATGCTGACGTGTAAGGAACGGAGGAGAGCAGCACGGCAGCAACAATCCAAAAAAGTTGGTG TGCGCTACTACGAGACACACAACGTGAAAAACAGGAACAGGAACAAAAAGAAGACGAGCGACTCAGAGGG ACAGAAACACAGACGCAACAAGTTCAGACAGAAGCAGTAACTGCGAGAAAGCTGTTTATTAAATTATACA AAAATAAAAAAAAAAAAAAAAA
Restriction Sites	EcoRI-NotI
ACCN	BC003262
Insert Size	2187 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC003262, AAH03262
RefSeq Size	2332 bp
RefSeq ORF	2187 bp
Locus ID	230737
Gene Summary	GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation (By similarity). May promote cell proliferation possibly by increasing p53/TP53 protein levels, and consequently those of its downstream product CDKN1A/p21, and decreasing RPL23A protein levels (By similarity).[UniProtKB/Swiss-Prot Function]

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