Wasl (BC055045) Mouse Untagged Clone
CAT＃: MC206401
Wasl (untagged) - Mouse Wiskott-Aldrich syndrome-like (human) (cDNA clone MGC:62799 IMAGE:6489610), (10ug)
CNY 3736.00
货期*
现货
规格
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Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 2900021I12Rik; 3110031I02Rik; N-WASP |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC055045
GGATTGTGCGGCTGCCGCCGCCGCCGCTGCCGCCGCCGCCGCCAGAGGACTGGAGGAGTAGGGAGTTTAG CGCAGCCGCCGGAGTGCTAGGACAACGACCATCCAGCCGGAGCTGAGCAGGGCGGGAGCCCGGAGCTTCC CTGTCGGCGCGCGGCCCGAGGGCGCCCGCCGTCATCTCCTTCCCTCGGCCTCCGCGCGCCGGAGGAGCGA CGACGGGATGCTGCGGCTGTGACCGCCGGCTCGCGCCCCTGTCACCCAGAGTGGGACCCGAGTGCTCGCC CGCCGCCAGAAGTGACGACCCTGGACGCTCCACCCCACCGGCGACACCATGAGCTCGGGCCAGCAGCCCC CGCGGAGGGTCACCAACGTGGGCTCCCTGCTGCTCACCCCGCAAGAGAACGAGTCTCTCTTCTCCTTCCT CGGCAAGAAATGTGTGACTATGTCTTCAGCAGTGGTGCAGTTGTATGCAGCAGATCGGAACTGTATGTGG GCAAAGAAGTGCAGTGGTGTCGCTTGTCTGGTTAAGGACAATCCTCAGAGATCTTTTTTTTTAAGAATAT TTGACATTAAGGATGGGAAATTACTGTGGGAACAAGAGCTATACAATAACTTTGTATATAATAGTCCTAG AGGATATTTTCATACCTTTGCTGGAGATACTTGTCAAGTAGCTCTTAATTTTGCCAATGAAGAAGAAGCA AAAAAGTTCCGAAAAGCAGTTACAGACCTGTTGGGCCGACGACAAAGGAAATCTGAAAAAAGACGAGATG CTCCAAATGGTCCCAATCTACCCATGGCTACAGTTGACATAAAAAATCCAGAAATCACAACAAATAGATT TTATGGTTCACAAGTCAACAACATCTCCCACACCAAAGAAAAGAAGAAAGGAAAAGCTAAAAAGAAGAGA TTAACCAAGGCAGATATTGGAACACCAAGTAATTTCCAGCACATTGGGCATGTTGGATGGGATCCAAATA CAGGTTTTGATCTAAATAATTTGGATCCAGAATTAAAGAATCTTTTTGATATGTGTGGGATCTCTGAGGC CCAGCTTAAAGACAGAGAAACATCAAAAGTTATTTATGACTTTATTGAAAAAACAGGAGGTGTAGAAGCT GTTAAAAACGAACTCCGAAGACAAGCACCACCACCACCTCCACCCTCGAGAGGAGGACCTCCCCCTCCTC CTCCCCCTCCTCATAGCTCAGGTCCTCCTCCCCCTCCTGCCCGAGGAAGGGGGGCTCCTCCTCCCCCACC TTCAAGAGCTCCTACTGCTGCACCTCCACCTCCACCTCCTTCTAGGCCTGGTGTTGTTGTTCCTCCGCCC CCTCCAAACAGGATGTACCCTCCTCCACCACCAGCGCTGCCCTCTTCAGCACCTTCAGGCCCTCCACCAC CTCCACCTCCATCTATGGCAGGGTCCACAGCACCACCACCTCCTCCTCCACCTCCGCCCCCACCAGGGCC ACCACCTCCCCCTGGCCTGCCTTCTGATGGTGACCATCAAGTTCCAGCTCCTTCAGGAAACAAAGCAGCT CTTTTGGATCAAATTAGAGAGGGTGCTCAGCTGAAAAAAGTGGAACAGAATAGCCGGCCCGTGTCCTGCT CAGGAAGGGATGCGCTTTTAGACCAGATACGACAGGGCATTCAATTGAAATCTGTGTCTGATGGCCAAGA GTCCACACCACCAACACCCGCACCCACTTCAGGAATTGTGGGTGCGCTGATGGAAGTGATGCAGAAAAGG AGCAAAGCCATTCATTCCTCAGATGAAGATGAAGACGATGATGATGAAGAAGATTTTGAGGATGATGATG AGTGGGAAGACTGATCTATATATTATATATATATATTTTTAAGGTGAAATACTAAACACTACTTCCTGTC TATTGATCTGTAAAATTATCATGTAAATGTTCTACCAATTGGCTGTATATTTTTGTTGCCTTTTTTGCTT TTTTTTTTTAAATTAATCTGTGCAATACCTCATTTAATCTGTAAAGGTTTGTTATAATCCTATATAAAGC TACTCCTTGTTGCTGTGTGCAAGTTTACATCAGGATGCTCACGTGATTTGTGAATATTTTCATTCCATCA ACAAGGGAGTTTAATTATGTGTGAGACTGACACAAACCTTAATGTAATTTAGTTAGAATGCCAGAAGGAG AACACTATTTTCATACCCTACTTTTTCTGTACCTAAATTTTCTTAATAAAATTTAGTATAGCACTACATT CTTTTTTAAGTGATGTAAACTTTAGTTTCTTTAGCCCCTTCATTTTGAATATGATGCCACATATGAATGT TGAAGCTGATACATTGCACAATTCTCTAGACACCACTCACTACACTAATGCAGTTTTTCAAATTCTAGTA TTATGTGTTGCTTAAGATCACTACAGAGATGCTACTGAAGAGAGACGGTAGTACAGCTCTCTTGCAGTAC TGCCTGCCGTTGGTAAGCAGTGGCATCCGAAGGCAGGAACCCTGATGAGCCCTCACGTGCCTGTCCTTGA CCAAGGTGACTTTTATGCTGTGGCAACTGTGACAGGCTATTTTTTAGCAAAGAGTTAGACGTTTGTTATC ATGCCCCAAAATAGTTTGAGGTAACATTTTTGGAGGTAGCTTCGTGCATCATAAACAACTTTCGTGCTCG TGTCTGACTCGAGCGGTATCAGAACAGGGCTGGGATGGAGTCTTCGTGTTTTGCACCCTGTGCATTGCTA TATTCACAAGTACCAACTGTAGGAGTCAGAGGTGTTGGCAAAAGTATGCATGAATGCTAACCTTTGCACA TACTCTGTTTTTACTTTGATGGCTTCTCCAGGTTTTTGTCAGGTACCACATGTAGAATTACATTTTTCTT AAAAGATTGATCATCCAAATGGCAAATTTCTTAGAGTCCATAACTGATTTGATAGATTTTTCTTTTTAAA TAAAACTTCACAGCCTGCACAGTTGTTACGCATACTACATGGTATGTTACAATTAGGGTTTCCTTGCCTC TTTACAGTACACTAATCCGCCTAAAGGTGGCTGTTTGATATTTACCGTGATAATTACCTTACCTACCTAC CTACTTTCAATACACTTTAGGTAGCCGATTATCTGATTTAAGTGCAGACACACTTTTCTCACATTGAGTT GTATGCAGAATGTAGCTTCAGATGTATTCATTATAGTTACAATATCCAACTAAAAAGTACGCTATCTAGA AGTATACCGACCAAAATCTAGTATTGACATGATCTTGACAGGCTGGACCTGTGAGATATGGTTTGAATTT TAACCAGTTTATCACATAACCTCTAGAGACCATGCATCTAGTTATTGTCAGGAATAATTTAAAAGGTTTA GTCGCCAAAAGCAGTTAAGTGGTGCAGTAAGATAGTTAAGTTATTCAAAGAGTGTTACCCTCCTTGCAAG AGTAACTGAAGCACATTGGAAAGATTCTCAATTTTGTGTTTCCTGTAAAAACAGTGCTCTCTGCTGCTAG GACCTTTTTTCTGCTTCCTGTCATTTTCATGTGGCTTGAGCTCTGAGACGTGCGGATGAGGCTGGACAAC TACTACCCATACAATTAGGGGGTCAAAATAACTTTAAATAAATAGCATCGGACTTTCATATTTTTCTTTT TGACGTGGAGAAAACATTTTCATGCCTAAAAATGTCTTTGAGATAATGCCAATTCTGATGTCCCTTACCT TTTGGCCAAGAAGTTTGAGATTTTTTCAGCTAATTTGTTATTTGACCAGAAGTTGTCTGGGGAGACTGAG TTCTTTGAGTTGCATATAAATGGGGGTATATTATTTGCCAAAGGTAAAATTGAGTTTATTCTTTTTCATA CAGAAATGGATTTTGCTTTTGTTTGCTTTTTATTTTTGTGTAAAATCTAAATTCCAGGAGCTATGGGGTT CATCCAGGCATGAAGGCTAATCCACTCTGAGTAAAGTGTCAGTTGCACAGCTCCGTCCTCTTCTGTCTTG CACTGTGGGTGGCACTATTTATTCCTGTGCGTCATCTGGAAGCCTCACGGATACATCCAGCTATGTGCTT GTCTGGTAGCCTTTGCGTTTAACAAACTAGCATGAGGCCTTTCATATCTAAATGCTGTGTGATAGACAAT TTCAGCTAGCCACATATTGTATTATGAAATTCATAAAGATTTTCAATCATTCAATTTCATTTATCAACTG AAATTTTGTTTAGTATGTGAATTTTTTTTTGAAATGTATAGTAAATAAGATGTTGCACTGGTGGCAATTC ATCATGGAACATAATAAAATTAATTTGACCCAAATAGTATAAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | BC055045 |
| Insert Size | 1506 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC055045, AAH55045 |
| RefSeq Size | 4325 bp |
| RefSeq ORF | 1506 bp |
| Locus ID | 73178 |
| Gene Summary | Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Involved in various processes, such as mitosis and cytokinesis, via its role in the regulation of actin polymerization. Together with CDC42, involved in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia. In addition to its role in the cytoplasm, also plays a role in the nucleus by regulating gene transcription, probably by promoting nuclear actin polymerization (By similarity). Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression (PubMed:12871950). Plays a role in dendrite spine morphogenesis (PubMed:25851601).[UniProtKB/Swiss-Prot Function] |
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