Anapc4 (BC024870) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2610306D21Rik; APC4; D5Ertd249e
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC024870 GGCCGCTGGCTAGGAGTAGGCCGAGTCTGCGACTCTGGCAGGTACCGTTGCTGGCAGGGCTTTGTGCCAG AGCTTTGTGGCCGGGTGCTCCGGCGGCCTTCTCGCGCACGTCGTTGCAGGGCCGCACCCATGCTGCGCTT TCCGACCTGTTTCCCATCCTTTCGGGTGGTGGGAGAGAAGCAGCTGCCACAGGAGATTATTTTCTTGGCC TGGTCCCCCAAGCGAGACCTCATTGCTTTGGCCAACACTACAGGCGAGGTTTTGCTGCATCGCCTTGCAA GTTTTCATCGAGTTTGGAGTTTCCCACCAAATGAAAGTACAGGAAAAGAAGTGACCTGCTTGGCCTGGAG ACCAGACGGCAAACTTTTGGCCTTTGCTCTTGCGGATACCAAGAAAATTATTTTGTGTGATGTAGAAAAG CCTGAAAGCTTACACTCCTTCTCTGTGGAGGCTCCGGTCTCTTGTATGCATTGGACAGAAGTGACTGTGG AAAGCAGTGTTTTAACATCGTTTTATAATGCTGAGGATGAGTCCAATCTTCTCTTGCCTAAGCTGCCCAC ATTGCCAAAAAATTACAACAGCACCTCAAAAATATTTAGTGAAGAAAATTCTGATGAAATTATCAAGCTC TTGGGAGATGTCAGGCTGAACATCCTTGTCCTTGGAGGAAGCTCTGGATTTATTGAGCTTTATGCTTATG GGATGTTCAAAATTGCCCGAGTAACAGGGATTGCCGGAACTTGTATTGCTCTGTGTTTATCAAGTGATTT GAAGTCATTATCAGTGGTCACAGAGGTTTCCAGCGGTGGCGAATCAGAAGTTTCATATTTTCAGCTTGAA ACCAATCTGCTGTATTCCTTCTTACCTGAAGTAACTCGTATGGCCAGGAAGTTTACTCACATTTCAGCTC TCTTACAGTATATAAATTTGTCACTGACATGTATGTGTGAAGCATGGGAAGAAATTCTGATGCAGATGGA CTCCCGTCTTACCAAGTTTGTACAGGAAAAACCTACAACCACGTCAGTGCAGGATGAGTTCATGCACTTG CTGCTGTGGGGGAAAGCCAGTGCTGAACTTCAGACTCTCCTGATGAACCAGTTAACAGTGAAGGGCTTAA AAAAGCTTGGCCAGTCTATAGAGTCATCATATTCCAGTATACAAAAACTGGTTATAAGTCACTTACAGAG TGGCTCTGAGTCTTTACTGTATCATTTGAGTGAACTTAAAGGAATGGCTTCATGGAAACAAAAATACGAA CCTCTAGGACTGGATGCTGCAGGCATTGAAGATGCTATTACTGCTGTGGGTTCTTTCATCCTCAAAGCAA ACGAACTTCTCCAAGTTATAGACAGTAGTATGAAAAACTTCAAGGCATTTTTTCGGTGGCTGTATGTAGC AATGCTGAGAATGACAGAGGACCATGTGCTTCCTGAGCTGAACAAGATGACTCAGAAGGATATCACATTT GTTGCTGAATTCCTCACTGAACATTTCAATGAGGCTCCAGACCTTTATAATAGGAAAGGAAAATACTTCA ATGTTGAAAGAGTTGGTCAGTATTTGAAAGATGAAGACGACGACCTTGTGTCACCCCCTAACACAGAAGG AAACCAGTGGTATGACTTCCTCCAAAACAGCACCCACCTGAAAGAAAGTCCTTTGCTGTTTCCTTATTAC CCTCGAAAATCATTGCATTTTGTGAAAAGGCGGATGGAGAACGTTATTGATCAGTGTTTGCAAAAGCCAG CAGATGTAATTGGAAGATCGATGAATCAAGCAATTTGCATTCCATTATATAAAGATGCTAGGAGTATGGA CTGTGCACGAAGATTGTTAAAATTCCCATTTCTGTGGAATAATAAAACTTCGAATCTTCATTATCTTCTT TTTACTATTTTAGAAGATTCGGTTTATAAAATGTGCATCTTAAGGAGACATACTGATATTTCCCAATCTG TAAGTAATGGACTAATTGGTATTAAATTTGGGAGCTTCACATCTGCCTCAGCTGACAAGGTCAGAAGAAG CTCTTACAGTTGTCTAGACGCCCAGTTCTATGATGATGAGACTGTCACGGTCATCCTGAAGGACTCCATG GGACGTGAAGGGAGAGACAGGATTTTGGTTCAGTTGTCACTGTCCTTGGTGTATAACAGTGAAGATTCTG ACGAATATGAGTTCACTGGGAGTTACTCGACAAGGCTTGACGAGCAGGGCAGCATTATCCCCACACGCAC CATGCACTTTGAGAAACACTGGAGGCTGCTAGAAAGCATGAGAGCGCAGTATGTTGCTGGGAACGGCCTT CGAAAAGTGTCCTGTGTGTTAAGTTCAAACCTCCGGCATGTGAGGGTGTTTGAAATGGACATCGATGATG AGTGGGAGATTGACGAGTCTTCAGACGATGAGGAGGAGGCTGGCGGGAAGCCTGTGAAGATCAAGGAGGA AGTGTTGTCAGAGTCAGAGACAGAGGCTCACCAAGACGCTGCTGCCCTAGACCCGGACGTAGTCATCAAA GTGGAGAAACTTGACCCTGAGTTGGACTCGTGACCCAGCTTGACAGCATTGTGTTAGTTAATGTGGCAAA AAGACGTAGTGGACGGACTAAGAATGTATCGGTCACACTGAGTTGTCTGTGTATCAAAGAAATAAATGGT AAATTTTAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC024870
Insert Size	2424 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC024870, AAH24870
RefSeq Size	2682 bp
RefSeq ORF	2424 bp
Locus ID	52206
Gene Summary	Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity).[UniProtKB/Swiss-Prot Function]

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