Ndc80 (BC020131) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	HEC, HEC1
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC020131 CGGACGCGTGGGTTCTAGATCGCGAGCGGCCGCTCGCGATCTAGAACTAGGGCGCCGGGTCTCGTTGAGA AGCTGAGCGAAAGGTTTCGAGAATGAAGCGCAGTTCAGTTTCCACCTGTGGTGCTGGCCGCCTCTCTATG CAGGAGTTAAGGACCCTGGACCTCAATAAGCCAGGCCTTTATACCCCTCAAACCAAAGAAAGATCAACCT TTGGAAAGCTGAGTACACACAAACCGACATCGGAAAGAAAAGTCTCAATATTTGGGAAAAGGACTAGCGG ACATGGATCCAGGAATAGTCAACTTGGTATATTTTCCAGTTCTGAAAAAATCAAGGACCCAAGACCACTT AATGACAAAGCATTCATTCAGCAGTGTATTCGACAACTCTATGAGTTTCTTACAGAAAACGGTTATGTGT ATAGTGTATCCATGAAGTCTCTGCAAGCTCCATCCACTAAAGAGTTCCTAAAGATCTTCGCCTTTCTTTA TGGCTTTCTGTGCCCGTCGTATGAACTTCCTGGTACAAAATGTGAAGAAGAGGTCCCAAGAATTTTTAAA GCACTTGGGTATCCCTTCACACTGTCCAAGAGCTCCATGTATACAGTGGGAGCCCCTCACACGTGGCCTC ACATCGTGGCTGCCTTGGTGTGGCTCATAGACTGCATCAAGATTGATACTGCCATGAAAGAAAGCTCACC TTTATTTGATGATGGGCAGCTCTGGGGAGAAGAGACTGAAGATGGAATTAAACACAATAAGTTGTTTTTG GAGTACACCAAAAAGTGCTATGAGAAGTTCATGACCGGGGCCGACAGCTTTGAAGAAGAGGATGCTGAGC TGCAGGCGAAGCTGAAGGACTTGTACAAGGTAGATGCGTCTAAGCTGGAGTCACTCGAAGCAGAAAACAA AGCACTAAATGAACAGATTGCAAGACTGGAGGAGGAAAGAGAAAGAGAACCGAACCGTCTGATGTCATTG AAGAAACTGAAAGCGTCCTTACAAGCAGATGTTCAAAACTATAAAGCATACATGAGCAACTTGGAGTCTC ATTTAGCCGTCCTGAAACAGAAATCGAATAGTCTTGATGAAGAAATTGGTAGAGTAGAACAAGAATGTGA AACTGTTAAACAGGAAAACACTCGACTACAGAGTATCGTTGATAACCAGAAGTATTCAGTCGCTGACATT GAGAGAATAAATCATGAGAAAAATGAATTGCAGCAGACTATTAATAAATTAACCAAAGACCTGGAAGCCG AACAGCAACAGATGTGGAATGAAGAATTAAAATACGCAAGAGGCAAAGAGGCGATTGAAGCGCAGCTAGC GGAGTACCACAAGTTGGCTAGAAAATTAAAGCTTATCCCCAAAGGTGCTGAGAATTCCAAAGGTTACGAC TTTGAAATTAAGTTTAATCCTGAGGCGGGTGCCAACTGCCTTGTCAAATACAGGACTCAAGTGTATGCAC CGCTCAAAGAGCTCTTGAATGAAAGCGAAGAAGAAATTAACAAAGCTCTGAATAAAAAGAGGCATCTGGA GGATACTTTAGAACAACTGAACACCATGAAAACGGAAAGCAAGAACACTGTGAGGATGCTGAAGGAGGAG ATTCAGAAACTGGATGACCTTCACCAGCAGGCAGTGAAGGAAGCTGAGGAAAAAGACAAGAAGAGTGCCA GTGAGCTTGAGTCCCTGGAGAAACACAAGCACCTGCTGGAGAGCGGGGTGAACGATGGCCTCAGCGAGGC CATGGATGAGTTGGACGCCGTCCAGCGGGAATACCAGCTAACTGTGAAGACCACAACTGAAGAAAGAAGA AAGGTGGAAAACAACTTACAACGTCTTTTGGAGATGGTCGCCACACACGTAGGGTCTTTGGAGAAACATC TTGAAGAGGAGAATGCTAAAGCCGACAGAGAGTATGAAGAATTCATGTCTGAAGATCTCCTGGAAAACAT CAGGGAGATGGCAGAGAAGTATAAGAGAAATGCTGCCCAACTTAAGGCTCCCGACAAATGAAGATAAACT AATCTTTCATAGATATAACATAGTATTTTATCCCCAAATTATGATAATATGGTAAATTGACCTTTTAGAC AGATCAGACAGTTAATAAAGTATACTTGTAAAGTGAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC020131
Insert Size	1929 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC020131, AAH20131
RefSeq Size	2150 bp
RefSeq ORF	1929 bp
Locus ID	67052
Gene Summary	Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules. Plays a role in chromosome congression and is essential for the end-on attachment of the kinetochores to spindle microtubules.[UniProtKB/Swiss-Prot Function]

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